Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus

Detalhes bibliográficos
Autor(a) principal: Cleare, Levi G.
Data de Publicação: 2020
Outros Autores: Zamith, Daniel, Heyman, Heino M., Couvillion, Sneha P., Nimrichter, Leonardo, Rodrigues, Marcio L., Nakayasu, Ernesto S., Nosanchuk, Joshua D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59380
Resumo: Department of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.
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spelling Cleare, Levi G.Zamith, DanielHeyman, Heino M.Couvillion, Sneha P.Nimrichter, LeonardoRodrigues, Marcio L.Nakayasu, Ernesto S.Nosanchuk, Joshua D.2023-07-03T21:00:25Z2023-07-03T21:00:25Z2020CLEARE, Levi G. et al. Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus. Cellular Microbiology, v. 22, n. 9, p. 1-32, 2020.1462-5814https://www.arca.fiocruz.br/handle/icict/5938010.1111/CMI.132171462-5822engWiley-Blackwellhttps://www.arca.fiocruz.br/handle/icict/59387Culture MediaExtracellular VesiclesFungal ProteinsHistoplasmaNutrientsBHICulture mediaDimorphic fungusExtracellular vesiclesHam's F12Histoplasma capsulatumHistoplasmosisLipidomicsMetabolomicsProteomicsRPMIUSAHistoplasma-capsulatumLipidsMedia matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDepartment of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.Department of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.Department of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA / Biological Sciences Division, Pacific Northwest National Laboratory. Richland. USA / Bruker Daltonics Inc. Billerica, USA.Biological Sciences Division. Pacific Northwest National Laboratory. Richland, USA.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, Brazil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.Biological Sciences Division. Pacific Northwest National Laboratory. Richland, USA.Department of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil.Histoplasma capsulatum is a dimorphic fungus that most frequently causes pneumonia, but can also disseminate and proliferate in diverse tissues. Histoplasma capsulatum has a complex secretion system that mediates the release of macromolecule-degrading enzymes and virulence factors. The formation and release of extracellular vesicles (EVs) are an important mechanism for non-conventional secretion in both ascomycetes and basidiomycetes. Histoplasma capsulatum EVs contain diverse proteins associated with virulence and are immunologically active. Despite the growing knowledge of EVs from H. capsulatum and other pathogenic fungi, the extent that changes in the environment impact the sorting of organic molecules in EVs has not been investigated. In this study, we cultivated H. capsulatum with distinct culture media to investigate the potential plasticity in EV loading in response to differences in nutrition. Our findings reveal that nutrition plays an important role in EV loading and formation, which may translate into differences in biological activities of these fungi in various fluids and tissues.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59380/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL10.1111_cmi.13217.pdfapplication/pdf1391859https://www.arca.fiocruz.br/bitstream/icict/59380/2/10.1111_cmi.13217.pdfc954a45c2736b43320a45f77de7ce165MD52icict/593802023-07-05 21:27:10.874oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-06T00:27:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus
title Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus
spellingShingle Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus
Cleare, Levi G.
Culture Media
Extracellular Vesicles
Fungal Proteins
Histoplasma
Nutrients
BHI
Culture media
Dimorphic fungus
Extracellular vesicles
Ham's F12
Histoplasma capsulatum
Histoplasmosis
Lipidomics
Metabolomics
Proteomics
RPMI
USA
Histoplasma-capsulatum
Lipids
title_short Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus
title_full Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus
title_fullStr Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus
title_full_unstemmed Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus
title_sort Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus
author Cleare, Levi G.
author_facet Cleare, Levi G.
Zamith, Daniel
Heyman, Heino M.
Couvillion, Sneha P.
Nimrichter, Leonardo
Rodrigues, Marcio L.
Nakayasu, Ernesto S.
Nosanchuk, Joshua D.
author_role author
author2 Zamith, Daniel
Heyman, Heino M.
Couvillion, Sneha P.
Nimrichter, Leonardo
Rodrigues, Marcio L.
Nakayasu, Ernesto S.
Nosanchuk, Joshua D.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cleare, Levi G.
Zamith, Daniel
Heyman, Heino M.
Couvillion, Sneha P.
Nimrichter, Leonardo
Rodrigues, Marcio L.
Nakayasu, Ernesto S.
Nosanchuk, Joshua D.
dc.subject.mesh.en_US.fl_str_mv Culture Media
Extracellular Vesicles
Fungal Proteins
Histoplasma
Nutrients
topic Culture Media
Extracellular Vesicles
Fungal Proteins
Histoplasma
Nutrients
BHI
Culture media
Dimorphic fungus
Extracellular vesicles
Ham's F12
Histoplasma capsulatum
Histoplasmosis
Lipidomics
Metabolomics
Proteomics
RPMI
USA
Histoplasma-capsulatum
Lipids
dc.subject.en.en_US.fl_str_mv BHI
Culture media
Dimorphic fungus
Extracellular vesicles
Ham's F12
Histoplasma capsulatum
Histoplasmosis
Lipidomics
Metabolomics
Proteomics
RPMI
USA
Histoplasma-capsulatum
Lipids
description Department of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2023-07-03T21:00:25Z
dc.date.available.fl_str_mv 2023-07-03T21:00:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CLEARE, Levi G. et al. Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus. Cellular Microbiology, v. 22, n. 9, p. 1-32, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59380
dc.identifier.issn.en_US.fl_str_mv 1462-5814
dc.identifier.doi.none.fl_str_mv 10.1111/CMI.13217
dc.identifier.eissn.none.fl_str_mv 1462-5822
identifier_str_mv CLEARE, Levi G. et al. Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus. Cellular Microbiology, v. 22, n. 9, p. 1-32, 2020.
1462-5814
10.1111/CMI.13217
1462-5822
url https://www.arca.fiocruz.br/handle/icict/59380
dc.language.iso.fl_str_mv eng
language eng
dc.relation.hasversion.none.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59387
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59380/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/59380/2/10.1111_cmi.13217.pdf
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