Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15316 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brasil |
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Mendes, GrazieleGonçalves, Vivian NicolauFagundes, Elaine Maria de SouzaKohlhoff, MarkusRosa, Carlos AugustoZani, Carlos LeomarCota, Betania BarrosRosa, Luiz HenriqueJohann, Susana2016-08-22T14:05:49Z2016-08-22T14:05:49Z2016MENDES, Graziele et al. Antifungal activity of extracts from Atacama Desert fungi againstParacoccidioides brasiliensis and identification ofAspergillus felis as a promising source of natural bioactive compounds. Mem. Inst. Oswaldo Cruz, v. 111, n. 3, p. 209-217, 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/1531610.1590/0074-02760150451engFundação Oswaldo CruzAntifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compoundsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilFungi of the genus Paracoccidioides are responsible for paracoccidioidomycosis. The occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. Compounds extracted from fungal extract have showing antifungal activity. Extracts of 78 fungi isolated from rocks of the Atacama Desert were tested in a microdilution assay against Paracoccidioides brasiliensis Pb18. Approximately 18% (5) of the extracts showed minimum inhibitory concentration (MIC) values≤ 125.0 µg/mL. Among these, extract from the fungus UFMGCB 8030 demonstrated the best results, with an MIC of 15.6 µg/mL. This isolate was identified as Aspergillus felis (by macro and micromorphologies, and internal transcribed spacer, β-tubulin, and ribosomal polymerase II gene analyses) and was grown in five different culture media and extracted with various solvents to optimise its antifungal activity. Potato dextrose agar culture and dichloromethane extraction resulted in an MIC of 1.9 µg/mL against P. brasiliensis and did not show cytotoxicity at the concentrations tested in normal mammalian cell (Vero). This extract was subjected to bioassay-guided fractionation using analytical C18RP-high-performance liquid chromatography (HPLC) and an antifungal assay using P. brasiliensis. Analysis of the active fractions by HPLC-high resolution mass spectrometry allowed us to identify the antifungal agents present in the A. felis extracts cytochalasins. These results reveal the potential of A. felis as a producer of bioactive compounds with antifungal activity.Rock-inhabiting FungiAtacama DesertParacoccidioides brasiliensisAntifungalAspergillus Felisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15316/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Mendes_Graziele_ Antifungal_CPqRR_2016.pdfve_Mendes_Graziele_ Antifungal_CPqRR_2016.pdfapplication/pdf614207https://www.arca.fiocruz.br/bitstream/icict/15316/2/ve_Mendes_Graziele_%20Antifungal_CPqRR_2016.pdf63480c12960be7ef23f94df4e4a6d7daMD52TEXTve_Mendes_Graziele_ Antifungal_CPqRR_2016.pdf.txtve_Mendes_Graziele_ Antifungal_CPqRR_2016.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds |
| title |
Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds |
| spellingShingle |
Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds Mendes, Graziele Rock-inhabiting Fungi Atacama Desert Paracoccidioides brasiliensis Antifungal Aspergillus Felis |
| title_short |
Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds |
| title_full |
Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds |
| title_fullStr |
Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds |
| title_full_unstemmed |
Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds |
| title_sort |
Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds |
| author |
Mendes, Graziele |
| author_facet |
Mendes, Graziele Gonçalves, Vivian Nicolau Fagundes, Elaine Maria de Souza Kohlhoff, Markus Rosa, Carlos Augusto Zani, Carlos Leomar Cota, Betania Barros Rosa, Luiz Henrique Johann, Susana |
| author_role |
author |
| author2 |
Gonçalves, Vivian Nicolau Fagundes, Elaine Maria de Souza Kohlhoff, Markus Rosa, Carlos Augusto Zani, Carlos Leomar Cota, Betania Barros Rosa, Luiz Henrique Johann, Susana |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mendes, Graziele Gonçalves, Vivian Nicolau Fagundes, Elaine Maria de Souza Kohlhoff, Markus Rosa, Carlos Augusto Zani, Carlos Leomar Cota, Betania Barros Rosa, Luiz Henrique Johann, Susana |
| dc.subject.en.pt_BR.fl_str_mv |
Rock-inhabiting Fungi Atacama Desert Paracoccidioides brasiliensis Antifungal Aspergillus Felis |
| topic |
Rock-inhabiting Fungi Atacama Desert Paracoccidioides brasiliensis Antifungal Aspergillus Felis |
| description |
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brasil |
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2016 |
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2016-08-22T14:05:49Z |
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2016-08-22T14:05:49Z |
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2016 |
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MENDES, Graziele et al. Antifungal activity of extracts from Atacama Desert fungi againstParacoccidioides brasiliensis and identification ofAspergillus felis as a promising source of natural bioactive compounds. Mem. Inst. Oswaldo Cruz, v. 111, n. 3, p. 209-217, 2016. |
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https://www.arca.fiocruz.br/handle/icict/15316 |
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0074-0276 |
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10.1590/0074-02760150451 |
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MENDES, Graziele et al. Antifungal activity of extracts from Atacama Desert fungi againstParacoccidioides brasiliensis and identification ofAspergillus felis as a promising source of natural bioactive compounds. Mem. Inst. Oswaldo Cruz, v. 111, n. 3, p. 209-217, 2016. 0074-0276 10.1590/0074-02760150451 |
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