PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil

Detalhes bibliográficos
Autor(a) principal: Sousa, Diego Rayan Teixeira de
Data de Publicação: 2015
Outros Autores: Santos, Carla Silvana da Silva, Wanke, Bodo, Silva Júnior, Roberto Moreira da, Santos, Marcelo Cordeiro dos, Cruz, Kátia Santana, Monte, Rossicléia Lins, Nocker, Andreas, Souza, João Vicente Braga de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34855
Resumo: 2020-08-16
id CRUZ_eef7ea075e07bd2199e9424569bdd161
oai_identifier_str oai:www.arca.fiocruz.br:icict/34855
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Sousa, Diego Rayan Teixeira deSantos, Carla Silvana da SilvaWanke, BodoSilva Júnior, Roberto Moreira daSantos, Marcelo Cordeiro dosCruz, Kátia SantanaMonte, Rossicléia LinsNocker, AndreasSouza, João Vicente Braga de2019-08-16T15:04:05Z2019-08-16T15:04:05Z2015SOUSA, Diego Rayan Teixeira de et al. PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil. Electronic Journal of Biotechnology, v. 18, p. 231-235, 2015.https://www.arca.fiocruz.br/handle/icict/3485510.1016/j.ejbt.2015.03.0120717-3458engElsevierPCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-08-16Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Pesquisa. Laboratório de Micologia. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Departamento de Pesquisa. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Pesquisa. Laboratório de Micologia. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Pesquisa. Laboratório de Micologia. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Pesquisa. Laboratório de Micologia. Manaus, AM, Brasil.Cranfield University. Water Sciences Institute. Cranfield, Bedfordshire, United Kingdom.Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil / Instituto Nacional de Pesquisas da Amazônia. Coordenação de Sociedade, Ambiente e Saúde. Laboratório de Micologia. Manaus, AM, Brasil.Background: The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses. In addition, the new approach was applied to detect fungal agents in 120 biological samples (blood, cerebrospinal fluid and bone marrow). PCR-RFLP results were compared with the ones obtained with conventional methods (culture, microscopy, and biochemical testing).Results: The assays carried out with the reference strains (Candida albicans, Candida parapsilosis, Candida tropicalis, Candida krusei, Candida guilliermondii, Cryptococcus neoformans, Cryptococcus gattii and Histoplasma capsulatum), demonstrated that the RFLP profiles were correctly predicted by thein silico investigation and allowed unequivocal identification of all chosen reference strains. The PCR-RFLP also identified 90 cultures of agents of invasive mycoses correctly, 2.5 times faster than the conventional assays. Evaluating PCR-RFLP with biological samples it was observed that the PCR was found to be 100% accurate and the RFLP profiles allowed the identification of the etiological agents: C. neoformans (n = 3) and C. gattii (n = 1) in CSF samples, H.capsulatum (n = 1) in bone marrow and C. albicans(n = 2) in blood cultures. The detection and identification by PCR-RFLP were found to be between two to ten times faster than the conventional assays. Conclusion: The results showed that PCR-RFLP is a valuable tool for the identification of invasive mycoses that canbe implemented in hospital laboratories, allowing for a high number of clinical analyses per day.Pathogenic fungusPolymorphismSimultaneous analysisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/34855/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Sousa_Diego_etal_INI_2015.pdfve_Sousa_Diego_etal_INI_2015.pdfapplication/pdf266957https://www.arca.fiocruz.br/bitstream/icict/34855/2/ve_Sousa_Diego_etal_INI_2015.pdfe38da6e09f747c5282f28ce8a73ae693MD52TEXTve_Sousa_Diego_etal_INI_2015.pdf.txtve_Sousa_Diego_etal_INI_2015.pdf.txtExtracted texttext/plain25711https://www.arca.fiocruz.br/bitstream/icict/34855/3/ve_Sousa_Diego_etal_INI_2015.pdf.txt47aad0c8c698f6e55c18a75d47617e6eMD53icict/348552021-02-10 19:38:17.197oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-02-10T22:38:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil
title PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil
spellingShingle PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil
Sousa, Diego Rayan Teixeira de
Pathogenic fungus
Polymorphism
Simultaneous analysis
title_short PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil
title_full PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil
title_fullStr PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil
title_full_unstemmed PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil
title_sort PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil
author Sousa, Diego Rayan Teixeira de
author_facet Sousa, Diego Rayan Teixeira de
Santos, Carla Silvana da Silva
Wanke, Bodo
Silva Júnior, Roberto Moreira da
Santos, Marcelo Cordeiro dos
Cruz, Kátia Santana
Monte, Rossicléia Lins
Nocker, Andreas
Souza, João Vicente Braga de
author_role author
author2 Santos, Carla Silvana da Silva
Wanke, Bodo
Silva Júnior, Roberto Moreira da
Santos, Marcelo Cordeiro dos
Cruz, Kátia Santana
Monte, Rossicléia Lins
Nocker, Andreas
Souza, João Vicente Braga de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Diego Rayan Teixeira de
Santos, Carla Silvana da Silva
Wanke, Bodo
Silva Júnior, Roberto Moreira da
Santos, Marcelo Cordeiro dos
Cruz, Kátia Santana
Monte, Rossicléia Lins
Nocker, Andreas
Souza, João Vicente Braga de
dc.subject.en.pt_BR.fl_str_mv Pathogenic fungus
Polymorphism
Simultaneous analysis
topic Pathogenic fungus
Polymorphism
Simultaneous analysis
description 2020-08-16
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2019-08-16T15:04:05Z
dc.date.available.fl_str_mv 2019-08-16T15:04:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUSA, Diego Rayan Teixeira de et al. PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil. Electronic Journal of Biotechnology, v. 18, p. 231-235, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34855
dc.identifier.doi.none.fl_str_mv 10.1016/j.ejbt.2015.03.012
dc.identifier.eissn.none.fl_str_mv 0717-3458
identifier_str_mv SOUSA, Diego Rayan Teixeira de et al. PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil. Electronic Journal of Biotechnology, v. 18, p. 231-235, 2015.
10.1016/j.ejbt.2015.03.012
0717-3458
url https://www.arca.fiocruz.br/handle/icict/34855
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/34855/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/34855/2/ve_Sousa_Diego_etal_INI_2015.pdf
https://www.arca.fiocruz.br/bitstream/icict/34855/3/ve_Sousa_Diego_etal_INI_2015.pdf.txt
bitstream.checksum.fl_str_mv 8a4605be74aa9ea9d79846c1fba20a33
e38da6e09f747c5282f28ce8a73ae693
47aad0c8c698f6e55c18a75d47617e6e
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324756076822528

Registros relacionados