PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/34855 |
Resumo: | 2020-08-16 |
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Sousa, Diego Rayan Teixeira deSantos, Carla Silvana da SilvaWanke, BodoSilva Júnior, Roberto Moreira daSantos, Marcelo Cordeiro dosCruz, Kátia SantanaMonte, Rossicléia LinsNocker, AndreasSouza, João Vicente Braga de2019-08-16T15:04:05Z2019-08-16T15:04:05Z2015SOUSA, Diego Rayan Teixeira de et al. PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil. Electronic Journal of Biotechnology, v. 18, p. 231-235, 2015.https://www.arca.fiocruz.br/handle/icict/3485510.1016/j.ejbt.2015.03.0120717-3458engElsevierPCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-08-16Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Pesquisa. Laboratório de Micologia. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Departamento de Pesquisa. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Pesquisa. Laboratório de Micologia. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Pesquisa. Laboratório de Micologia. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Pesquisa. Laboratório de Micologia. Manaus, AM, Brasil.Cranfield University. Water Sciences Institute. Cranfield, Bedfordshire, United Kingdom.Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil / Instituto Nacional de Pesquisas da Amazônia. Coordenação de Sociedade, Ambiente e Saúde. Laboratório de Micologia. Manaus, AM, Brasil.Background: The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses. In addition, the new approach was applied to detect fungal agents in 120 biological samples (blood, cerebrospinal fluid and bone marrow). PCR-RFLP results were compared with the ones obtained with conventional methods (culture, microscopy, and biochemical testing).Results: The assays carried out with the reference strains (Candida albicans, Candida parapsilosis, Candida tropicalis, Candida krusei, Candida guilliermondii, Cryptococcus neoformans, Cryptococcus gattii and Histoplasma capsulatum), demonstrated that the RFLP profiles were correctly predicted by thein silico investigation and allowed unequivocal identification of all chosen reference strains. The PCR-RFLP also identified 90 cultures of agents of invasive mycoses correctly, 2.5 times faster than the conventional assays. Evaluating PCR-RFLP with biological samples it was observed that the PCR was found to be 100% accurate and the RFLP profiles allowed the identification of the etiological agents: C. neoformans (n = 3) and C. gattii (n = 1) in CSF samples, H.capsulatum (n = 1) in bone marrow and C. albicans(n = 2) in blood cultures. The detection and identification by PCR-RFLP were found to be between two to ten times faster than the conventional assays. Conclusion: The results showed that PCR-RFLP is a valuable tool for the identification of invasive mycoses that canbe implemented in hospital laboratories, allowing for a high number of clinical analyses per day.Pathogenic fungusPolymorphismSimultaneous analysisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/34855/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Sousa_Diego_etal_INI_2015.pdfve_Sousa_Diego_etal_INI_2015.pdfapplication/pdf266957https://www.arca.fiocruz.br/bitstream/icict/34855/2/ve_Sousa_Diego_etal_INI_2015.pdfe38da6e09f747c5282f28ce8a73ae693MD52TEXTve_Sousa_Diego_etal_INI_2015.pdf.txtve_Sousa_Diego_etal_INI_2015.pdf.txtExtracted texttext/plain25711https://www.arca.fiocruz.br/bitstream/icict/34855/3/ve_Sousa_Diego_etal_INI_2015.pdf.txt47aad0c8c698f6e55c18a75d47617e6eMD53icict/348552021-02-10 19:38:17.197oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-02-10T22:38:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil |
title |
PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil |
spellingShingle |
PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil Sousa, Diego Rayan Teixeira de Pathogenic fungus Polymorphism Simultaneous analysis |
title_short |
PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil |
title_full |
PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil |
title_fullStr |
PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil |
title_full_unstemmed |
PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil |
title_sort |
PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare facility in the North of Brazil |
author |
Sousa, Diego Rayan Teixeira de |
author_facet |
Sousa, Diego Rayan Teixeira de Santos, Carla Silvana da Silva Wanke, Bodo Silva Júnior, Roberto Moreira da Santos, Marcelo Cordeiro dos Cruz, Kátia Santana Monte, Rossicléia Lins Nocker, Andreas Souza, João Vicente Braga de |
author_role |
author |
author2 |
Santos, Carla Silvana da Silva Wanke, Bodo Silva Júnior, Roberto Moreira da Santos, Marcelo Cordeiro dos Cruz, Kátia Santana Monte, Rossicléia Lins Nocker, Andreas Souza, João Vicente Braga de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sousa, Diego Rayan Teixeira de Santos, Carla Silvana da Silva Wanke, Bodo Silva Júnior, Roberto Moreira da Santos, Marcelo Cordeiro dos Cruz, Kátia Santana Monte, Rossicléia Lins Nocker, Andreas Souza, João Vicente Braga de |
dc.subject.en.pt_BR.fl_str_mv |
Pathogenic fungus Polymorphism Simultaneous analysis |
topic |
Pathogenic fungus Polymorphism Simultaneous analysis |
description |
2020-08-16 |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2019-08-16T15:04:05Z |
dc.date.available.fl_str_mv |
2019-08-16T15:04:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUSA, Diego Rayan Teixeira de et al. PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil. Electronic Journal of Biotechnology, v. 18, p. 231-235, 2015. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/34855 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.ejbt.2015.03.012 |
dc.identifier.eissn.none.fl_str_mv |
0717-3458 |
identifier_str_mv |
SOUSA, Diego Rayan Teixeira de et al. PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil. Electronic Journal of Biotechnology, v. 18, p. 231-235, 2015. 10.1016/j.ejbt.2015.03.012 0717-3458 |
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https://www.arca.fiocruz.br/handle/icict/34855 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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