The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs

Detalhes bibliográficos
Autor(a) principal: Fraga, Deborah Bittencourt Mothé
Data de Publicação: 2016
Outros Autores: Pacheco, Luciano Vasconcellos, Borja, Lairton Souza, Tuy, Pétala Gardênia da Silva Estrela, Bastos, Leila Andrade, Solcà, Manuela da Silva, Amorim, Leila Denise Alves Ferreira, Veras, Patrícia Sampaio Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13876
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, Brasil
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spelling Fraga, Deborah Bittencourt MothéPacheco, Luciano VasconcellosBorja, Lairton SouzaTuy, Pétala Gardênia da Silva EstrelaBastos, Leila AndradeSolcà, Manuela da SilvaAmorim, Leila Denise Alves FerreiraVeras, Patrícia Sampaio Tavares2016-04-19T18:07:54Z2016-04-19T18:07:54Z2016FRAGA, D. B. M. et al. The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs. PLOS Neglected Tropical Diseases, v. 10, n. 1, e0004333, 2016.1935-272710.1371/journal.pntd.0004333https://www.arca.fiocruz.br/handle/icict/13876engPublic Library of ScienceLeishmaniaLeishmaniose visceralAnimalCãesSaúde públicaCélulasDiagnósticoThe Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, BrasilVisceral Leishmaniasis (VL) has spread to many urban centers worldwide. Dogs are considered the main reservoir of VL, because canine cases often precede the occurrence of human cases. Detection and euthanasia of serologically positive dogs is one of the primary VL control measures utilized in some countries, including Brazil. Using accurate diagnostic tests can minimize one undesirable consequence of this measure, culling false-positive dogs, and reduce the maintenance of false-negative dogs in endemic areas. In December 2011, the Brazilian Ministry of Health replaced the ELISA (EIE CVL) screening method and Indirect Immunofluorescence Test (IFI CVL) confirmatory method with a new protocol using the rapid DPP CVL screening test and EIE CVL confirmatory test. A study of diagnostic accuracy of these two protocols was done by comparing their performance using serum samples collected from a random sample of 780 dogs in an endemic area of VL. All samples were evaluated by culture and real time PCR; 766 out of the 780 dogs were tested using the previous protocol (IFI CVL + EIE CVL) and all 780 were tested using the current protocol (DPP CVL + EIE CVL). Performances of both diagnostic protocols were evaluated using a latent class variable as the gold standard. The current protocol had a higher specificity (0.98 vs. 0.95) and PPV (0.83 vs. 0.70) than the previous protocol, although sensitivity of these two protocols was similar (0.73). When tested using sera from asymptomatic animals, the current protocol had a much higher PPV (0.63 vs. 0.40) than the previous protocol (although the sensitivity of either protocol was the same, 0.71). Considering a range of theoretical CVL prevalences, the projected PPVs were higher for the current protocol than for the previous protocol for each theoretical prevalence value. The findings presented herein show that the current protocol performed better than previous protocol primarily by reducing false-positive results.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13876/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFraga DBM The rapid test....pdfFraga DBM The rapid test....pdfapplication/pdf284308https://www.arca.fiocruz.br/bitstream/icict/13876/2/Fraga%20DBM%20The%20rapid%20test....pdf1c2bbeb546df6c762a78bb88d0d04e58MD52TEXTFraga DBM The rapid test....pdf.txtFraga DBM The rapid test....pdf.txtExtracted texttext/plain41680https://www.arca.fiocruz.br/bitstream/icict/13876/3/Fraga%20DBM%20The%20rapid%20test....pdf.txt4b18f75c12158a938a02512537c61a37MD53icict/138762023-03-15 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dc.title.pt_BR.fl_str_mv The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs
title The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs
spellingShingle The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs
Fraga, Deborah Bittencourt Mothé
Leishmania
Leishmaniose visceral
Animal
Cães
Saúde pública
Células
Diagnóstico
title_short The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs
title_full The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs
title_fullStr The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs
title_full_unstemmed The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs
title_sort The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs
author Fraga, Deborah Bittencourt Mothé
author_facet Fraga, Deborah Bittencourt Mothé
Pacheco, Luciano Vasconcellos
Borja, Lairton Souza
Tuy, Pétala Gardênia da Silva Estrela
Bastos, Leila Andrade
Solcà, Manuela da Silva
Amorim, Leila Denise Alves Ferreira
Veras, Patrícia Sampaio Tavares
author_role author
author2 Pacheco, Luciano Vasconcellos
Borja, Lairton Souza
Tuy, Pétala Gardênia da Silva Estrela
Bastos, Leila Andrade
Solcà, Manuela da Silva
Amorim, Leila Denise Alves Ferreira
Veras, Patrícia Sampaio Tavares
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fraga, Deborah Bittencourt Mothé
Pacheco, Luciano Vasconcellos
Borja, Lairton Souza
Tuy, Pétala Gardênia da Silva Estrela
Bastos, Leila Andrade
Solcà, Manuela da Silva
Amorim, Leila Denise Alves Ferreira
Veras, Patrícia Sampaio Tavares
dc.subject.other.pt_BR.fl_str_mv Leishmania
Leishmaniose visceral
Animal
Cães
Saúde pública
Células
Diagnóstico
topic Leishmania
Leishmaniose visceral
Animal
Cães
Saúde pública
Células
Diagnóstico
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, Brasil
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-04-19T18:07:54Z
dc.date.available.fl_str_mv 2016-04-19T18:07:54Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRAGA, D. B. M. et al. The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs. PLOS Neglected Tropical Diseases, v. 10, n. 1, e0004333, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13876
dc.identifier.issn.none.fl_str_mv 1935-2727
10.1371/journal.pntd.0004333
identifier_str_mv FRAGA, D. B. M. et al. The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs. PLOS Neglected Tropical Diseases, v. 10, n. 1, e0004333, 2016.
1935-2727
10.1371/journal.pntd.0004333
url https://www.arca.fiocruz.br/handle/icict/13876
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/13876/3/Fraga%20DBM%20The%20rapid%20test....pdf.txt
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