The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13876 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, Brasil |
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Fraga, Deborah Bittencourt MothéPacheco, Luciano VasconcellosBorja, Lairton SouzaTuy, Pétala Gardênia da Silva EstrelaBastos, Leila AndradeSolcà, Manuela da SilvaAmorim, Leila Denise Alves FerreiraVeras, Patrícia Sampaio Tavares2016-04-19T18:07:54Z2016-04-19T18:07:54Z2016FRAGA, D. B. M. et al. The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs. PLOS Neglected Tropical Diseases, v. 10, n. 1, e0004333, 2016.1935-272710.1371/journal.pntd.0004333https://www.arca.fiocruz.br/handle/icict/13876engPublic Library of ScienceLeishmaniaLeishmaniose visceralAnimalCãesSaúde públicaCélulasDiagnósticoThe Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, BrasilVisceral Leishmaniasis (VL) has spread to many urban centers worldwide. Dogs are considered the main reservoir of VL, because canine cases often precede the occurrence of human cases. Detection and euthanasia of serologically positive dogs is one of the primary VL control measures utilized in some countries, including Brazil. Using accurate diagnostic tests can minimize one undesirable consequence of this measure, culling false-positive dogs, and reduce the maintenance of false-negative dogs in endemic areas. In December 2011, the Brazilian Ministry of Health replaced the ELISA (EIE CVL) screening method and Indirect Immunofluorescence Test (IFI CVL) confirmatory method with a new protocol using the rapid DPP CVL screening test and EIE CVL confirmatory test. A study of diagnostic accuracy of these two protocols was done by comparing their performance using serum samples collected from a random sample of 780 dogs in an endemic area of VL. All samples were evaluated by culture and real time PCR; 766 out of the 780 dogs were tested using the previous protocol (IFI CVL + EIE CVL) and all 780 were tested using the current protocol (DPP CVL + EIE CVL). Performances of both diagnostic protocols were evaluated using a latent class variable as the gold standard. The current protocol had a higher specificity (0.98 vs. 0.95) and PPV (0.83 vs. 0.70) than the previous protocol, although sensitivity of these two protocols was similar (0.73). When tested using sera from asymptomatic animals, the current protocol had a much higher PPV (0.63 vs. 0.40) than the previous protocol (although the sensitivity of either protocol was the same, 0.71). Considering a range of theoretical CVL prevalences, the projected PPVs were higher for the current protocol than for the previous protocol for each theoretical prevalence value. The findings presented herein show that the current protocol performed better than previous protocol primarily by reducing false-positive results.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13876/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFraga DBM The rapid test....pdfFraga DBM The rapid test....pdfapplication/pdf284308https://www.arca.fiocruz.br/bitstream/icict/13876/2/Fraga%20DBM%20The%20rapid%20test....pdf1c2bbeb546df6c762a78bb88d0d04e58MD52TEXTFraga DBM The rapid test....pdf.txtFraga DBM The rapid test....pdf.txtExtracted texttext/plain41680https://www.arca.fiocruz.br/bitstream/icict/13876/3/Fraga%20DBM%20The%20rapid%20test....pdf.txt4b18f75c12158a938a02512537c61a37MD53icict/138762023-03-15 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dc.title.pt_BR.fl_str_mv |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs |
title |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs |
spellingShingle |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs Fraga, Deborah Bittencourt Mothé Leishmania Leishmaniose visceral Animal Cães Saúde pública Células Diagnóstico |
title_short |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs |
title_full |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs |
title_fullStr |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs |
title_full_unstemmed |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs |
title_sort |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs |
author |
Fraga, Deborah Bittencourt Mothé |
author_facet |
Fraga, Deborah Bittencourt Mothé Pacheco, Luciano Vasconcellos Borja, Lairton Souza Tuy, Pétala Gardênia da Silva Estrela Bastos, Leila Andrade Solcà, Manuela da Silva Amorim, Leila Denise Alves Ferreira Veras, Patrícia Sampaio Tavares |
author_role |
author |
author2 |
Pacheco, Luciano Vasconcellos Borja, Lairton Souza Tuy, Pétala Gardênia da Silva Estrela Bastos, Leila Andrade Solcà, Manuela da Silva Amorim, Leila Denise Alves Ferreira Veras, Patrícia Sampaio Tavares |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fraga, Deborah Bittencourt Mothé Pacheco, Luciano Vasconcellos Borja, Lairton Souza Tuy, Pétala Gardênia da Silva Estrela Bastos, Leila Andrade Solcà, Manuela da Silva Amorim, Leila Denise Alves Ferreira Veras, Patrícia Sampaio Tavares |
dc.subject.other.pt_BR.fl_str_mv |
Leishmania Leishmaniose visceral Animal Cães Saúde pública Células Diagnóstico |
topic |
Leishmania Leishmaniose visceral Animal Cães Saúde pública Células Diagnóstico |
description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, Brasil |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-04-19T18:07:54Z |
dc.date.available.fl_str_mv |
2016-04-19T18:07:54Z |
dc.date.issued.fl_str_mv |
2016 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FRAGA, D. B. M. et al. The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs. PLOS Neglected Tropical Diseases, v. 10, n. 1, e0004333, 2016. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/13876 |
dc.identifier.issn.none.fl_str_mv |
1935-2727 10.1371/journal.pntd.0004333 |
identifier_str_mv |
FRAGA, D. B. M. et al. The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs. PLOS Neglected Tropical Diseases, v. 10, n. 1, e0004333, 2016. 1935-2727 10.1371/journal.pntd.0004333 |
url |
https://www.arca.fiocruz.br/handle/icict/13876 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Public Library of Science |
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Public Library of Science |
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Repositório Institucional da FIOCRUZ (ARCA) |
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