Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase

Detalhes bibliográficos
Autor(a) principal: Reis, Mitermayer Galvão dos
Data de Publicação: 1993
Outros Autores: Davis, Richard E, Singh, Hardeep, Skelly, Patrick J, Shoemaker, Charles B
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7580
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Harvard School of Public Health. Department of Tropical Public Health. Boston, MA, USA
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spelling Reis, Mitermayer Galvão dosDavis, Richard ESingh, HardeepSkelly, Patrick JShoemaker, Charles B2014-05-05T19:24:49Z2014-05-05T19:24:49Z1993REIS, M. G dos et al. Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase. Molecular Biochemical Parasitology, v. 59, n. 2, p. 235-242, 1993.0166-6851https://www.arca.fiocruz.br/handle/icict/7580engElsevier Science Publishers B.VCharacterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomeraseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Harvard School of Public Health. Department of Tropical Public Health. Boston, MA, USADepartment of Biology. San Francisco State University. San Francisco, CA, USADepartment of Biology. San Francisco State University. San Francisco, CA, USAHarvard School of Public Health. Department of Tropical Public Health. Boston, MA, USAHarvard School of Public Health.Department of Tropical Public Health. Boston, MA, USAThe complete gene encoding Schistosoma mansoni triosephosphate isomerase (TPI) was isolated from a lambda phage genomic library on 2 overlapping clones. These genomic clones have been characterized by restriction mapping and DNA sequencing of the 5' flanking region, the exons, the intron boundaries and the polyadenylation addition site. S. mansoni TPI is encoded by 6 exons spanning a region of about 12 kb. The 5 introns are located at positions precisely analogous to those of mammalian TPI genes but one of the 6 mammalian TPI introns is missing in S. mansoni. We find no evidence of spliced leader involvement in TPI gene expression. The gene is preceded by at least 4 tandem copies of a 2.5-kb repetitive sequence. While the 12-kb size for the S. mansoni TPI gene is much larger than the 3-4 kb typical of mammalian TPI genes, the 42-bp first intron is unusually short. The transcription initiation site for the S. mansoni TPI gene is heterogeneous. Genomic Southern blot analysis suggests that TPI is expressed from a single copy gene.SchistosomeSchistosoma mansoniTPIGlycolysisIntronGeneAnimaisSchistosoma mansoni/enzimologiaSchistosoma mansoni/genéticaTriose-Fosfato Isomerase/genéticaSequência de AminoácidosNorthern BlottingClonagem MolecularDNA/análiseDNA/isolamento & purificaçãoSequência de BasesBiblioteca GenômicaGlicóliseDados de Sequência MolecularOligodesoxirribonucleotídeosReação em Cadeia da PolimeraseRNA Mensageiro/isolamento & purificaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALReis MG Characterization of the schistosoma....pdfReis MG Characterization of the schistosoma....pdfapplication/pdf948929https://www.arca.fiocruz.br/bitstream/icict/7580/1/Reis%20MG%20Characterization%20of%20the%20schistosoma....pdf272d3c948b9e6581e328050c421d4af8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7580/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTReis MG Characterization of the schistosoma....pdf.txtReis MG Characterization of the schistosoma....pdf.txtExtracted texttext/plain31904https://www.arca.fiocruz.br/bitstream/icict/7580/5/Reis%20MG%20Characterization%20of%20the%20schistosoma....pdf.txt03a726cf77a69c026c5a277a4661060eMD55THUMBNAILReis MG Characterization of the schistosoma....pdf.jpgReis MG Characterization of the schistosoma....pdf.jpgGenerated Thumbnailimage/jpeg2454https://www.arca.fiocruz.br/bitstream/icict/7580/4/Reis%20MG%20Characterization%20of%20the%20schistosoma....pdf.jpgf8a6bb82d1b62dd3f594fe75708f6b98MD54icict/75802023-03-15 14:33:18.481oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase
title Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase
spellingShingle Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase
Reis, Mitermayer Galvão dos
Schistosome
Schistosoma mansoni
TPI
Glycolysis
Intron
Gene
Animais
Schistosoma mansoni/enzimologia
Schistosoma mansoni/genética
Triose-Fosfato Isomerase/genética
Sequência de Aminoácidos
Northern Blotting
Clonagem Molecular
DNA/análise
DNA/isolamento & purificação
Sequência de Bases
Biblioteca Genômica
Glicólise
Dados de Sequência Molecular
Oligodesoxirribonucleotídeos
Reação em Cadeia da Polimerase
RNA Mensageiro/isolamento & purificação
title_short Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase
title_full Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase
title_fullStr Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase
title_full_unstemmed Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase
title_sort Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase
author Reis, Mitermayer Galvão dos
author_facet Reis, Mitermayer Galvão dos
Davis, Richard E
Singh, Hardeep
Skelly, Patrick J
Shoemaker, Charles B
author_role author
author2 Davis, Richard E
Singh, Hardeep
Skelly, Patrick J
Shoemaker, Charles B
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Reis, Mitermayer Galvão dos
Davis, Richard E
Singh, Hardeep
Skelly, Patrick J
Shoemaker, Charles B
dc.subject.en.pt_BR.fl_str_mv Schistosome
Schistosoma mansoni
TPI
Glycolysis
Intron
Gene
Animais
topic Schistosome
Schistosoma mansoni
TPI
Glycolysis
Intron
Gene
Animais
Schistosoma mansoni/enzimologia
Schistosoma mansoni/genética
Triose-Fosfato Isomerase/genética
Sequência de Aminoácidos
Northern Blotting
Clonagem Molecular
DNA/análise
DNA/isolamento & purificação
Sequência de Bases
Biblioteca Genômica
Glicólise
Dados de Sequência Molecular
Oligodesoxirribonucleotídeos
Reação em Cadeia da Polimerase
RNA Mensageiro/isolamento & purificação
dc.subject.decs.pt_BR.fl_str_mv Schistosoma mansoni/enzimologia
Schistosoma mansoni/genética
Triose-Fosfato Isomerase/genética
Sequência de Aminoácidos
Northern Blotting
Clonagem Molecular
DNA/análise
DNA/isolamento & purificação
Sequência de Bases
Biblioteca Genômica
Glicólise
Dados de Sequência Molecular
Oligodesoxirribonucleotídeos
Reação em Cadeia da Polimerase
RNA Mensageiro/isolamento & purificação
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Harvard School of Public Health. Department of Tropical Public Health. Boston, MA, USA
publishDate 1993
dc.date.issued.fl_str_mv 1993
dc.date.accessioned.fl_str_mv 2014-05-05T19:24:49Z
dc.date.available.fl_str_mv 2014-05-05T19:24:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REIS, M. G dos et al. Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase. Molecular Biochemical Parasitology, v. 59, n. 2, p. 235-242, 1993.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7580
dc.identifier.issn.none.fl_str_mv 0166-6851
identifier_str_mv REIS, M. G dos et al. Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase. Molecular Biochemical Parasitology, v. 59, n. 2, p. 235-242, 1993.
0166-6851
url https://www.arca.fiocruz.br/handle/icict/7580
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Science Publishers B.V
publisher.none.fl_str_mv Elsevier Science Publishers B.V
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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