A selective medium for the isolation of the Acinetobacter genus bacteria

Detalhes bibliográficos
Autor(a) principal: Zebral, Altair A.
Data de Publicação: 1980
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42033
Resumo: Um meio seletivo e diferenciador foi desenvolvido para facilitar o isolamento das bactérias do gênero Acinetobacter. Este meio (Agar Acinetobacter) contém em gramas por litro: Tiotone (BBL), 10; extrato de levedura (BBL), 3; NaC1, 5; sacarose, 10; manitol, 10; citrato de sódio, 0,5; desoxicolato de sódio, 0,1; cristal violeta, 0,00025; vermelho de fenol, 0,04 e pH 7,4. Apresenta a vantagem de impedir o crescimento dos cocos e bacilos Gram positivos, pelo emprego do citrato de sódio e do desoxicolato de sódio, associados ao cristal violeta e de diferenciar os bacilos Gram negativos da família Enterobacteriaceae, pela sua atividade fermentativa sobre a sacarose e/ou manitol, em contraste com a completa inatividade das bactérias do gênero Acinetobacter sobre os mesmos substratos.
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spelling Zebral, Altair A.2020-07-01T19:56:48Z2020-07-01T19:56:48Z1980ZEBRAL, Altair; A selective medium for the isolation of the acinetobacter genus bacteria. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 3-4, p. 11-21, jul./dez. 1980.0074-0276https://www.arca.fiocruz.br/handle/icict/4203310.1590/S0074-027619800002000021678-8060Um meio seletivo e diferenciador foi desenvolvido para facilitar o isolamento das bactérias do gênero Acinetobacter. Este meio (Agar Acinetobacter) contém em gramas por litro: Tiotone (BBL), 10; extrato de levedura (BBL), 3; NaC1, 5; sacarose, 10; manitol, 10; citrato de sódio, 0,5; desoxicolato de sódio, 0,1; cristal violeta, 0,00025; vermelho de fenol, 0,04 e pH 7,4. Apresenta a vantagem de impedir o crescimento dos cocos e bacilos Gram positivos, pelo emprego do citrato de sódio e do desoxicolato de sódio, associados ao cristal violeta e de diferenciar os bacilos Gram negativos da família Enterobacteriaceae, pela sua atividade fermentativa sobre a sacarose e/ou manitol, em contraste com a completa inatividade das bactérias do gênero Acinetobacter sobre os mesmos substratos.A selective and differencial medium was developed for the isolation of Acinetobacter genus bacteria. This Acinobacter Agar Medium (p.H + 7.4) contains in grams per litre: thiotone, 10; yeast extract, 3; naC1, 5; saccharose, 10; mannitol, 10; sodium citrate, 0.5; sodium desoxycholate, 0.1; crystal violet, 0.00025; phenol red, 0.04 and agar-agar 15. This medium has the advantage of inhibiting the growth of cocci and Gram-positive bacilli, by the use of sodium citrate and sodium desoxycholate associated with the crystal violet; and of differentiating the Gram-negative bacilli from the Enterobacteriaceae, through the fermentative activity upon the saccharose and/or mannitol, contrasting with the complete inactivity of the Acinetobacter genus bacteria over those substances.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.engFundação Oswaldo Cruz. 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dc.title.pt_BR.fl_str_mv A selective medium for the isolation of the Acinetobacter genus bacteria
title A selective medium for the isolation of the Acinetobacter genus bacteria
spellingShingle A selective medium for the isolation of the Acinetobacter genus bacteria
Zebral, Altair A.
Agar Acinetobacter
Isolamento
Acinobacter Agar Medium
title_short A selective medium for the isolation of the Acinetobacter genus bacteria
title_full A selective medium for the isolation of the Acinetobacter genus bacteria
title_fullStr A selective medium for the isolation of the Acinetobacter genus bacteria
title_full_unstemmed A selective medium for the isolation of the Acinetobacter genus bacteria
title_sort A selective medium for the isolation of the Acinetobacter genus bacteria
author Zebral, Altair A.
author_facet Zebral, Altair A.
author_role author
dc.contributor.author.fl_str_mv Zebral, Altair A.
dc.subject.other.pt_BR.fl_str_mv Agar Acinetobacter
Isolamento
topic Agar Acinetobacter
Isolamento
Acinobacter Agar Medium
dc.subject.en.pt_BR.fl_str_mv Acinobacter Agar Medium
description Um meio seletivo e diferenciador foi desenvolvido para facilitar o isolamento das bactérias do gênero Acinetobacter. Este meio (Agar Acinetobacter) contém em gramas por litro: Tiotone (BBL), 10; extrato de levedura (BBL), 3; NaC1, 5; sacarose, 10; manitol, 10; citrato de sódio, 0,5; desoxicolato de sódio, 0,1; cristal violeta, 0,00025; vermelho de fenol, 0,04 e pH 7,4. Apresenta a vantagem de impedir o crescimento dos cocos e bacilos Gram positivos, pelo emprego do citrato de sódio e do desoxicolato de sódio, associados ao cristal violeta e de diferenciar os bacilos Gram negativos da família Enterobacteriaceae, pela sua atividade fermentativa sobre a sacarose e/ou manitol, em contraste com a completa inatividade das bactérias do gênero Acinetobacter sobre os mesmos substratos.
publishDate 1980
dc.date.issued.fl_str_mv 1980
dc.date.accessioned.fl_str_mv 2020-07-01T19:56:48Z
dc.date.available.fl_str_mv 2020-07-01T19:56:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ZEBRAL, Altair; A selective medium for the isolation of the acinetobacter genus bacteria. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 3-4, p. 11-21, jul./dez. 1980.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42033
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761980000200002
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv ZEBRAL, Altair; A selective medium for the isolation of the acinetobacter genus bacteria. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 3-4, p. 11-21, jul./dez. 1980.
0074-0276
10.1590/S0074-02761980000200002
1678-8060
url https://www.arca.fiocruz.br/handle/icict/42033
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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