Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases

Detalhes bibliográficos
Autor(a) principal: Farias, João Ramalho Ortigão
Data de Publicação: 2018
Outros Autores: Blasi, Tatiana Di, Telleria, Erich Loza, Andorinho, Ana Carolina, Silva, Thais Lemos, Ortigão, Marcelo Ramalho, Tempone, Antônio Jorge, Traub-Csekö, Yara Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26242
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.
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spelling Farias, João Ramalho OrtigãoBlasi, Tatiana DiTelleria, Erich LozaAndorinho, Ana CarolinaSilva, Thais LemosOrtigão, Marcelo RamalhoTempone, Antônio JorgeTraub-Csekö, Yara Maria2018-05-03T15:16:08Z2018-05-03T15:16:08Z2018FARIAS, João Ramalho Ortigão; et al. Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases. Mem Inst Oswaldo Cruz, v.113, n.2, p.96-101, Feb. 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/2624210.1590/0074-027601701791678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzLutzomyia longipalpisQuitinasesProcessamento Alternativodomínio de ligação de quitinaLutzomyia longipalpischitinasealternative splicingchitin binding domainAlternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.BACKGROUND The insect chitinase gene family is composed by more than 10 paralogs, which can codify proteins with different domain structures. In Lutzomyia longipalpis, the main vector of visceral leishmaniasis in Brazil, a chitinase cDNA from adult female insects was previously characterized. The predicted protein contains one catalytic domain and one chitin-binding domain (CBD). The expression of this gene coincided with the end of blood digestion indicating a putative role in peritrophic matrix degradation. OBJECTIVES To determine the occurrence of alternative splicing in chitinases of L. longipalpis. METHODS We sequenced the LlChit1 gene from a genomic clone and the three spliced forms obtained by reverse transcription polymerase chain reaction (RT-PCR) using larvae cDNA. FINDINGS We showed that LlChit1 from L. longipalpis immature forms undergoes alternative splicing. The spliced form corresponding to the adult cDNA was named LlChit1A and the two larvae specific transcripts were named LlChit1B and LlChit1C. The B and C forms possess stop codons interrupting the translation of the CBD. The A form is present in adult females post blood meal, L4 larvae and pre-pupae, while the other two forms are present only in L4 larvae and disappear just before pupation. Two bands of the expected size were identified by Western blot only in L4 larvae. MAIN CONCLUSIONS We show for the first time alternative splicing generating chitinases with different domain structures increasing our understanding on the finely regulated digestion physiology and shedding light on a potential target for controlling L. longipalpis larval development.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26242/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjoaoramalho_farias_etal_IOC_2018.pdfjoaoramalho_farias_etal_IOC_2018.pdfapplication/pdf1758359https://www.arca.fiocruz.br/bitstream/icict/26242/2/joaoramalho_farias_etal_IOC_2018.pdf50852ac82a05fb3c454a7d195a99204bMD52TEXTjoaoramalho_farias_etal_IOC_2018.pdf.txtjoaoramalho_farias_etal_IOC_2018.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases
title Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases
spellingShingle Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases
Farias, João Ramalho Ortigão
Lutzomyia longipalpis
Quitinases
Processamento Alternativo
domínio de ligação de quitina
Lutzomyia longipalpis
chitinase
alternative splicing
chitin binding domain
title_short Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases
title_full Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases
title_fullStr Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases
title_full_unstemmed Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases
title_sort Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases
author Farias, João Ramalho Ortigão
author_facet Farias, João Ramalho Ortigão
Blasi, Tatiana Di
Telleria, Erich Loza
Andorinho, Ana Carolina
Silva, Thais Lemos
Ortigão, Marcelo Ramalho
Tempone, Antônio Jorge
Traub-Csekö, Yara Maria
author_role author
author2 Blasi, Tatiana Di
Telleria, Erich Loza
Andorinho, Ana Carolina
Silva, Thais Lemos
Ortigão, Marcelo Ramalho
Tempone, Antônio Jorge
Traub-Csekö, Yara Maria
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Farias, João Ramalho Ortigão
Blasi, Tatiana Di
Telleria, Erich Loza
Andorinho, Ana Carolina
Silva, Thais Lemos
Ortigão, Marcelo Ramalho
Tempone, Antônio Jorge
Traub-Csekö, Yara Maria
dc.subject.other.pt_BR.fl_str_mv Lutzomyia longipalpis
Quitinases
Processamento Alternativo
domínio de ligação de quitina
topic Lutzomyia longipalpis
Quitinases
Processamento Alternativo
domínio de ligação de quitina
Lutzomyia longipalpis
chitinase
alternative splicing
chitin binding domain
dc.subject.en.pt_BR.fl_str_mv Lutzomyia longipalpis
chitinase
alternative splicing
chitin binding domain
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitos e Vetores. Rio de Janeiro, RJ. Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-05-03T15:16:08Z
dc.date.available.fl_str_mv 2018-05-03T15:16:08Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIAS, João Ramalho Ortigão; et al. Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases. Mem Inst Oswaldo Cruz, v.113, n.2, p.96-101, Feb. 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26242
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760170179
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv FARIAS, João Ramalho Ortigão; et al. Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases. Mem Inst Oswaldo Cruz, v.113, n.2, p.96-101, Feb. 2018.
0074-0276
10.1590/0074-02760170179
1678-8060
url https://www.arca.fiocruz.br/handle/icict/26242
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/26242/2/joaoramalho_farias_etal_IOC_2018.pdf
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