Genomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS

Detalhes bibliográficos
Autor(a) principal: Morgado, Sergio Mascarenhas
Data de Publicação: 2020
Outros Autores: Vicente, Ana Carolina Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45045
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular e Mricroorganismos. Rio de Janeiro, RJ, Brasil.
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spelling Morgado, Sergio MascarenhasVicente, Ana Carolina Paulo2020-12-18T18:32:43Z2020-12-18T18:32:43Z2020MORGADO, Sergio Mascarenhas; VICENTE, Ana Carolina Paulo. Genomics of Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS. Microbiology Society, v. 6, n. 7, mgen000382, p. 1-11, July 2020.1465-2080https://www.arca.fiocruz.br/handle/icict/4504510.1099/mgen.0.000382engMicrobiology SocietyMycobacteriumMycobacteriaceaeMycobacteriumMycobacteriaceaeICET7SST4SSGenomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SSinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular e Mricroorganismos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular e Mricroorganismos. Rio de Janeiro, RJ, Brasil.Mobile genetic elements (MGEs) are agents of bacterial evolution and adaptation. Genome sequencing provides an unbiased approach that has revealed an abundance of MGEs in prokaryotes, mainly plasmids and integrative conjugative elements. Nevertheless, many mobilomes, particularly those from environmental bacteria, remain underexplored despite their representing a reservoir of genes that can later emerge in the clinic. Here, we explored the mobilome of the Mycobacteriaceae family, focusing on strains from Brazilian Atlantic Forest soil. Novel Mycolicibacterium and Mycobacteroides strains were identified, with the former ones harbouring linear and circular plasmids encoding the specialized type-VII secretion system (T7SS) and mobility-associated genes. In addition, we also identified a T4SS-mediated integrative conjugative element (ICEMyc226) encoding two T7SSs and a number of xenobiotic degrading genes. Our study uncovers the diversity of the Mycobacteriaceae mobilome, providing the evidence of an ICE in this bacterial family. Moreover, the presence of T7SS genes in an ICE, as well as plasmids, highlights the role of these mobile genetic elements in the dispersion of T7SS.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45045/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAnaCarolinaPVicente_Sergioorgado_IOC_2020.pdfAnaCarolinaPVicente_Sergioorgado_IOC_2020.pdfapplication/pdf1582060https://www.arca.fiocruz.br/bitstream/icict/45045/2/AnaCarolinaPVicente_Sergioorgado_IOC_2020.pdf1cc3cd5b39441774ef8849412e4f1b8dMD52TEXTAnaCarolinaPVicente_Sergioorgado_IOC_2020.pdf.txtAnaCarolinaPVicente_Sergioorgado_IOC_2020.pdf.txtExtracted texttext/plain50200https://www.arca.fiocruz.br/bitstream/icict/45045/3/AnaCarolinaPVicente_Sergioorgado_IOC_2020.pdf.txtab4a074b1085004ee361b193a8e4e52eMD53icict/450452021-03-13 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dc.title.pt_BR.fl_str_mv Genomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS
title Genomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS
spellingShingle Genomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS
Morgado, Sergio Mascarenhas
Mycobacterium
Mycobacteriaceae
Mycobacterium
Mycobacteriaceae
ICE
T7SS
T4SS
title_short Genomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS
title_full Genomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS
title_fullStr Genomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS
title_full_unstemmed Genomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS
title_sort Genomics and Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS
author Morgado, Sergio Mascarenhas
author_facet Morgado, Sergio Mascarenhas
Vicente, Ana Carolina Paulo
author_role author
author2 Vicente, Ana Carolina Paulo
author2_role author
dc.contributor.author.fl_str_mv Morgado, Sergio Mascarenhas
Vicente, Ana Carolina Paulo
dc.subject.other.pt_BR.fl_str_mv Mycobacterium
Mycobacteriaceae
topic Mycobacterium
Mycobacteriaceae
Mycobacterium
Mycobacteriaceae
ICE
T7SS
T4SS
dc.subject.en.pt_BR.fl_str_mv Mycobacterium
Mycobacteriaceae
ICE
T7SS
T4SS
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular e Mricroorganismos. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-12-18T18:32:43Z
dc.date.available.fl_str_mv 2020-12-18T18:32:43Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORGADO, Sergio Mascarenhas; VICENTE, Ana Carolina Paulo. Genomics of Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS. Microbiology Society, v. 6, n. 7, mgen000382, p. 1-11, July 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45045
dc.identifier.issn.pt_BR.fl_str_mv 1465-2080
dc.identifier.doi.none.fl_str_mv 10.1099/mgen.0.000382
identifier_str_mv MORGADO, Sergio Mascarenhas; VICENTE, Ana Carolina Paulo. Genomics of Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS. Microbiology Society, v. 6, n. 7, mgen000382, p. 1-11, July 2020.
1465-2080
10.1099/mgen.0.000382
url https://www.arca.fiocruz.br/handle/icict/45045
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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