Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids

Detalhes bibliográficos
Autor(a) principal: Fernandes, Carlos A. H.
Data de Publicação: 2015
Outros Autores: Cardoso, Fábio Florença, Cavalcante, Walter G. L., Soares, Andreimar M., Dal-Pai, Maeli, Gallacci, Marcia, Fontes, Marcos R. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12527
Resumo: Universidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brazil. / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Instituto Nacional de Ciência e Tecnologia em Toxinas. São Paulo, SP, Brazil.
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spelling Fernandes, Carlos A. H.Cardoso, Fábio FlorençaCavalcante, Walter G. L.Soares, Andreimar M.Dal-Pai, MaeliGallacci, MarciaFontes, Marcos R. M.2016-01-13T21:36:49Z2016-01-13T21:36:49Z2015Fernandes C.A.H., et al. Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids. Plos One V.10, 2015https://www.arca.fiocruz.br/handle/icict/12527DOI:10.1371/journal.pone.0133370engPlos OneStructural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acidsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brazil. / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Instituto Nacional de Ciência e Tecnologia em Toxinas. São Paulo, SP, Brazil.Universidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brazil. / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Instituto Nacional de Ciência e Tecnologia em Toxinas. São Paulo, SP, Brazil. / Universidade Estadual Paulista. Instituto de Biociências. Departamento de Farmacologia. Botucatu, SP, Brazil.Universidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brazil. / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Instituto Nacional de Ciência e Tecnologia em Toxinas. São Paulo, SP, Brazil. / Universidade Estadual Paulista. Instituto de Biociências. Departamento de Farmacologia. Botucatu, SP, Brazil.Fundação Oswaldo Cruz. Porto Velho, RO, Brazil. / Universidade Federal de Rondônia. Centro de Estudos de Biomoléculas Aplicadas. Porto Velho, RO, Brazil.Universidade Estadual Paulista. Instituto de Biociências. Departamento de Morfologia. Botucatu, SP, Brazil.Universidade Estadual Paulista. Instituto de Biociências. Departamento de Farmacologia. Botucatu, SP, Brazil.Universidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brazil. / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Instituto Nacional de Ciência e Tecnologia em Toxinas. São Paulo, SP, Brazil.One of the main challenges in toxicology today is to develop therapeutic alternatives for the treatment of snake venom injuries that are not efficiently neutralized by conventional serum therapy. Venom phospholipases A2 (PLA2s) and PLA2-like proteins play a fundamental role in skeletal muscle necrosis, which can result in permanent sequelae and disability. This leads to economic and social problems, especially in developing countries. In this work, we performed structural and functional studies with Piratoxin-I, a Lys49-PLA2 from Bothropspirajai venom, complexed with two compounds present in several plants used in folk medicine against snakebites. These ligands partially neutralized the myotoxic activity of PrTX-I towards binding on the two independent sites of interaction between Lys49-PLA2 and muscle membrane. Our results corroborate the previously proposed mechanism of action of PLA2s-like and provide insights for the design of structure-based inhibitors that could prevent the permanent injuries caused by these proteins in snakebite victimsCaffeic and Aristolochic acidsBothrops pirajai piratoxin-Lys49-phospholipase A2-likeStructure-based inhibitorsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12527/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL4 - Structural Basis for the Inhibition.pdf4 - Structural Basis for the Inhibition.pdfapplication/pdf623628https://www.arca.fiocruz.br/bitstream/icict/12527/2/4%20-%20Structural%20Basis%20for%20the%20Inhibition.pdf38354b9d1e411223e4a85f0e90628559MD52TEXT4 - Structural Basis for the Inhibition.pdf.txt4 - Structural Basis for the Inhibition.pdf.txtExtracted texttext/plain59220https://www.arca.fiocruz.br/bitstream/icict/12527/3/4%20-%20Structural%20Basis%20for%20the%20Inhibition.pdf.txt402be343f8bb487a06c9aa6b939fd435MD53icict/125272018-04-02 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dc.title.pt_BR.fl_str_mv Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids
title Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids
spellingShingle Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids
Fernandes, Carlos A. H.
Caffeic and Aristolochic acids
Bothrops pirajai piratoxin-
Lys49-phospholipase A2-like
Structure-based inhibitors
title_short Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids
title_full Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids
title_fullStr Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids
title_full_unstemmed Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids
title_sort Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids
author Fernandes, Carlos A. H.
author_facet Fernandes, Carlos A. H.
Cardoso, Fábio Florença
Cavalcante, Walter G. L.
Soares, Andreimar M.
Dal-Pai, Maeli
Gallacci, Marcia
Fontes, Marcos R. M.
author_role author
author2 Cardoso, Fábio Florença
Cavalcante, Walter G. L.
Soares, Andreimar M.
Dal-Pai, Maeli
Gallacci, Marcia
Fontes, Marcos R. M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Carlos A. H.
Cardoso, Fábio Florença
Cavalcante, Walter G. L.
Soares, Andreimar M.
Dal-Pai, Maeli
Gallacci, Marcia
Fontes, Marcos R. M.
dc.subject.en.pt_BR.fl_str_mv Caffeic and Aristolochic acids
Bothrops pirajai piratoxin-
Lys49-phospholipase A2-like
Structure-based inhibitors
topic Caffeic and Aristolochic acids
Bothrops pirajai piratoxin-
Lys49-phospholipase A2-like
Structure-based inhibitors
description Universidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brazil. / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Instituto Nacional de Ciência e Tecnologia em Toxinas. São Paulo, SP, Brazil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-13T21:36:49Z
dc.date.available.fl_str_mv 2016-01-13T21:36:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Fernandes C.A.H., et al. Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids. Plos One V.10, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12527
dc.identifier.doi.none.fl_str_mv DOI:10.1371/journal.pone.0133370
identifier_str_mv Fernandes C.A.H., et al. Structural Basis for the Inhibition of a Phospholipase A2-Like Toxin by Caffeic and Aristolochic Acids. Plos One V.10, 2015
DOI:10.1371/journal.pone.0133370
url https://www.arca.fiocruz.br/handle/icict/12527
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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