Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50922 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. |
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Chaves, Lana BitencourtGuimarães, Glaucia de OliveiraSilva, Daiana de Souza Perce daBanic, Dalma MariaTotino, Paulo Renato RivasMachado, Ricardo Luiz DantasSilva, Rodrigo Nunes Rodrigues daPratt-Riccio, Lilian RoseRibeiro, Cláudio Tadeu DanielLima Junior, Josué da Costa2022-01-27T18:34:04Z2022-01-27T18:34:04Z2021CHAVES, Lana Bitencourt et al. Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon. Genes, v. 12, 1657, p. 1 - 18, Oct. 2021.2073-4425https://www.arca.fiocruz.br/handle/icict/5092210.3390/ genes12111657engMDPIMaláriaPlasmodium vivaxAmazônia brasileiraDiversidade genéticaPredictionCandidato a vacinaMalariaBrazilian AmazonPlasmodium vivaxPvCyRPAGenetic diversityPredictionVaccine candidateGenetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Centro Universitário Arthur Sá Earp Neto. Laboratório de Imunologia Básica e Aplicada. Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiologia. Laboratório de Anticorpos Monoclonais.Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.The Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) has an important role in erythrocyte invasion and has been considered a target for vivax malaria vaccine development. Nonetheless, its genetic diversity remains uncharted in Brazilian malaria-endemic areas. Therefore, we investigated the pvcyrpa genetic polymorphism in 98 field isolates from the Brazilian Amazon and its impact on the antigenicity of predicted B-cell epitopes. Genetic diversity parameters, population genetic analysis, neutrality test and the median-joining network were analyzed, and the potential amino acid polymorphism participation in B-cell epitopes was investigated. One synonymous and 26 non-synonymous substitutions defined fifty haplotypes. The nucleotide diversity and Tajima’s D values varied across the coding gene. The exon-1 sequence had greater diversity than those of exon-2. Concerning the prediction analysis, seven sequences were predicted as linear B cell epitopes, the majority contained in conformational epitopes. Moreover, important amino acid polymorphism was detected in regions predicted to contain residues participating in B-cell epitopes. Our data suggest that the pvcyrpa gene presents a moderate polymorphism in the studied isolates and such polymorphisms alter amino acid sequences contained in potential B cell epitopes, an important observation considering the antigen potentiality as a vaccine candidate to cover distinct P. vivax endemic areas worldwide.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50922/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLilianRicio_ClaudioTDRibeiro_etal_IOC_2021.pdfLilianRicio_ClaudioTDRibeiro_etal_IOC_2021.pdfapplication/pdf1455943https://www.arca.fiocruz.br/bitstream/icict/50922/2/LilianRicio_ClaudioTDRibeiro_etal_IOC_2021.pdfcfe1ac200672fbf21219029076dbb13aMD52icict/509222022-06-24 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| dc.title.pt_BR.fl_str_mv |
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title |
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| spellingShingle |
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon Chaves, Lana Bitencourt Malária Plasmodium vivax Amazônia brasileira Diversidade genética Prediction Candidato a vacina Malaria Brazilian Amazon Plasmodium vivax PvCyRPA Genetic diversity Prediction Vaccine candidate |
| title_short |
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title_full |
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title_fullStr |
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title_full_unstemmed |
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title_sort |
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| author |
Chaves, Lana Bitencourt |
| author_facet |
Chaves, Lana Bitencourt Guimarães, Glaucia de Oliveira Silva, Daiana de Souza Perce da Banic, Dalma Maria Totino, Paulo Renato Rivas Machado, Ricardo Luiz Dantas Silva, Rodrigo Nunes Rodrigues da Pratt-Riccio, Lilian Rose Ribeiro, Cláudio Tadeu Daniel Lima Junior, Josué da Costa |
| author_role |
author |
| author2 |
Guimarães, Glaucia de Oliveira Silva, Daiana de Souza Perce da Banic, Dalma Maria Totino, Paulo Renato Rivas Machado, Ricardo Luiz Dantas Silva, Rodrigo Nunes Rodrigues da Pratt-Riccio, Lilian Rose Ribeiro, Cláudio Tadeu Daniel Lima Junior, Josué da Costa |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Chaves, Lana Bitencourt Guimarães, Glaucia de Oliveira Silva, Daiana de Souza Perce da Banic, Dalma Maria Totino, Paulo Renato Rivas Machado, Ricardo Luiz Dantas Silva, Rodrigo Nunes Rodrigues da Pratt-Riccio, Lilian Rose Ribeiro, Cláudio Tadeu Daniel Lima Junior, Josué da Costa |
| dc.subject.other.pt_BR.fl_str_mv |
Malária Plasmodium vivax Amazônia brasileira Diversidade genética Prediction Candidato a vacina |
| topic |
Malária Plasmodium vivax Amazônia brasileira Diversidade genética Prediction Candidato a vacina Malaria Brazilian Amazon Plasmodium vivax PvCyRPA Genetic diversity Prediction Vaccine candidate |
| dc.subject.en.pt_BR.fl_str_mv |
Malaria Brazilian Amazon Plasmodium vivax PvCyRPA Genetic diversity Prediction Vaccine candidate |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2021 |
| dc.date.issued.fl_str_mv |
2021 |
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2022-01-27T18:34:04Z |
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2022-01-27T18:34:04Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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CHAVES, Lana Bitencourt et al. Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon. Genes, v. 12, 1657, p. 1 - 18, Oct. 2021. |
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https://www.arca.fiocruz.br/handle/icict/50922 |
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2073-4425 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/ genes12111657 |
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CHAVES, Lana Bitencourt et al. Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon. Genes, v. 12, 1657, p. 1 - 18, Oct. 2021. 2073-4425 10.3390/ genes12111657 |
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https://www.arca.fiocruz.br/handle/icict/50922 |
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MDPI |
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MDPI |
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