Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts

Detalhes bibliográficos
Autor(a) principal: Rizzo, Juliana
Data de Publicação: 2020
Outros Autores: Chaze, Thibault, Miranda, Kildare, Roberson, Robert W., Gorgette, Olivier, Nimrichter, Leonardo, Matondo, Mariette, Latgé, Jean-Paul, Beauvais, Anne, Rodrigues, Marcio Lourenço
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44098
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil. / Institut Pasteur. Département de Mycologie. Paris, France.
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spelling Rizzo, JulianaChaze, ThibaultMiranda, KildareRoberson, Robert W.Gorgette, OlivierNimrichter, LeonardoMatondo, MarietteLatgé, Jean-PaulBeauvais, AnneRodrigues, Marcio Lourenço2020-10-21T15:25:11Z2020-10-21T15:25:11Z2020RIZZO, Juliana et al. Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts. mSphere, v. 5, n. 4, p. 1-16, 2020.2379-5042https://www.arca.fiocruz.br/handle/icict/4409810.1128/ mSphere.00476-20porAmerican Society for MicrobiologyconidiaSpores, FungalProtoplastsExtracellular VesiclesEsporas FúngicasProtoplastosVesículas ExtracelularesSpores fongiquesProtoplastesVésicules extracellulairesAspergillus fumigatusEsporos FúngicosProtoplastosVesículas ExtracelularesCharacterization of extracellular vesicles produced by Aspergillus fumigatus protoplastsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil. / Institut Pasteur. Département de Mycologie. Paris, France.Institut Pasteur. Centre de Ressources et Recherches Technologiques. Unité de Spectrométrie de Masse pour la Biologie. Plateforme Protéomique. Paris, France.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil. / Universidade Federal do Rio de Janeiro. Centro Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.School of Life Sciences. Arizona State University. Tempe, Arizona, USA.Institut Pasteur. Plateforme de Microscopie Ultrastructurale. Imagepole. Paris, France.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Centre de Ressources et Recherches Technologiques. Unité de Spectrométrie de Masse pour la Biologie. Plateforme Protéomique. Paris, France.Institut Pasteur. Département de Mycologie. Paris, France. / School of Medicine. University of Crete. Heraklion, Crete, Greece.Institut Pasteur. Département de Mycologie. Paris, France.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Extracellular vesicles (EVs) are membranous compartments produced by yeast and mycelial forms of several fungal species. One of the difficulties in perceiving the role of EVs during the fungal life, and particularly in cell wall biogenesis, is caused by the presence of a thick cell wall. One alternative to have better access to these vesicles is to use protoplasts. This approach has been investigated here with Aspergillus fumigatus, one of the most common opportunistic fungal pathogens worldwide. Analysis of regenerating protoplasts by scanning electron microscopy and fluorescence microscopy indicated the occurrence of outer membrane projections in association with surface components and the release of particles with properties resembling those of fungal EVs. EVs in culture supernatants were characterized by transmission electron microscopy and nanoparticle tracking analysis. Proteomic and glycome analysis of EVs revealed the presence of a complex array of enzymes related to lipid/sugar metabolism, pathogenic processes, and cell wall biosynthesis. Our data indicate that (i) EV production is a common feature of different morphological stages of this major fungal pathogen and (ii) protoplastic EVs are promising tools for undertaking studies of vesicle functions in fungal cells. Is important know that Fungal cells use extracellular vesicles (EVs) to export biologically active molecules to the extracellular space. In this study, we used protoplasts of Aspergillus fumigatus, a major fungal pathogen, as a model to evaluate the role of EV production in cell wall biogenesis. Our results demonstrated that wall-less A. fumigatus exports plasma membrane-derived EVs containing a complex combination of proteins and glycans. Our report is the first to characterize fungal EVs in the absence of a cell wall. Our results suggest that protoplasts represent a promising model for functional studies of fungal vesicles.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/44098/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALmSphere-2020-Rizzo-e00476-20ok.pdfmSphere-2020-Rizzo-e00476-20ok.pdfapplication/pdf2647378https://www.arca.fiocruz.br/bitstream/icict/44098/2/mSphere-2020-Rizzo-e00476-20ok.pdf6f845085f2f2557f9b9070aadfd9bddaMD52TEXTmSphere-2020-Rizzo-e00476-20ok.pdf.txtmSphere-2020-Rizzo-e00476-20ok.pdf.txtExtracted texttext/plain75808https://www.arca.fiocruz.br/bitstream/icict/44098/3/mSphere-2020-Rizzo-e00476-20ok.pdf.txt88887dc482159beaa14ea90e63229189MD53icict/440982020-10-22 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dc.title.pt_BR.fl_str_mv Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts
title Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts
spellingShingle Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts
Rizzo, Juliana
conidia
Spores, Fungal
Protoplasts
Extracellular Vesicles
Esporas Fúngicas
Protoplastos
Vesículas Extracelulares
Spores fongiques
Protoplastes
Vésicules extracellulaires
Aspergillus fumigatus
Esporos Fúngicos
Protoplastos
Vesículas Extracelulares
title_short Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts
title_full Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts
title_fullStr Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts
title_full_unstemmed Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts
title_sort Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts
author Rizzo, Juliana
author_facet Rizzo, Juliana
Chaze, Thibault
Miranda, Kildare
Roberson, Robert W.
Gorgette, Olivier
Nimrichter, Leonardo
Matondo, Mariette
Latgé, Jean-Paul
Beauvais, Anne
Rodrigues, Marcio Lourenço
author_role author
author2 Chaze, Thibault
Miranda, Kildare
Roberson, Robert W.
Gorgette, Olivier
Nimrichter, Leonardo
Matondo, Mariette
Latgé, Jean-Paul
Beauvais, Anne
Rodrigues, Marcio Lourenço
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rizzo, Juliana
Chaze, Thibault
Miranda, Kildare
Roberson, Robert W.
Gorgette, Olivier
Nimrichter, Leonardo
Matondo, Mariette
Latgé, Jean-Paul
Beauvais, Anne
Rodrigues, Marcio Lourenço
dc.subject.other.pt_BR.fl_str_mv conidia
topic conidia
Spores, Fungal
Protoplasts
Extracellular Vesicles
Esporas Fúngicas
Protoplastos
Vesículas Extracelulares
Spores fongiques
Protoplastes
Vésicules extracellulaires
Aspergillus fumigatus
Esporos Fúngicos
Protoplastos
Vesículas Extracelulares
dc.subject.en.pt_BR.fl_str_mv Spores, Fungal
Protoplasts
Extracellular Vesicles
dc.subject.es.pt_BR.fl_str_mv Esporas Fúngicas
Protoplastos
Vesículas Extracelulares
dc.subject.fr.pt_BR.fl_str_mv Spores fongiques
Protoplastes
Vésicules extracellulaires
dc.subject.decs.pt_BR.fl_str_mv Aspergillus fumigatus
Esporos Fúngicos
Protoplastos
Vesículas Extracelulares
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil. / Institut Pasteur. Département de Mycologie. Paris, France.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-21T15:25:11Z
dc.date.available.fl_str_mv 2020-10-21T15:25:11Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIZZO, Juliana et al. Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts. mSphere, v. 5, n. 4, p. 1-16, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44098
dc.identifier.issn.pt_BR.fl_str_mv 2379-5042
dc.identifier.doi.none.fl_str_mv 10.1128/ mSphere.00476-20
identifier_str_mv RIZZO, Juliana et al. Characterization of extracellular vesicles produced by Aspergillus fumigatus protoplasts. mSphere, v. 5, n. 4, p. 1-16, 2020.
2379-5042
10.1128/ mSphere.00476-20
url https://www.arca.fiocruz.br/handle/icict/44098
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/44098/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/44098/2/mSphere-2020-Rizzo-e00476-20ok.pdf
https://www.arca.fiocruz.br/bitstream/icict/44098/3/mSphere-2020-Rizzo-e00476-20ok.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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