Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31554 |
Resumo: | Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil. |
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Reymão, Tammy Kathlyn AmaralFumian, Tulio MachadoJustino, Maria Cleonice AguiarHernandez, Juliana MercesBandeira, Renato SilvaLucena, Maria Silvia SousaTeixeira, Dielle MonteiroFarias, Fredison PinheiroSilva, Luciana DamascenaLinhares, Alexandre CostaGabbay, Yvone Benchimol2019-02-12T11:12:19Z2019-02-12T11:12:19Z2018REYMÃO, Tammy Kathlyn Amaral; et al. Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis. Plos One, v.13, n.7, e0199763, 14p, July 2018.1932-6203https://www.arca.fiocruz.br/handle/icict/3155410.1371/journal.pone.0199763engPublic Library of ScienceRNA de NorovírusSoroCarga viral fecalCriançasAnálise molecularNorovirus RNAFecal viral loadSerumChildrenMolecular analysisNorovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brasil.Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.Worldwide, norovirus (NoV) is a major cause of acute gastroenteritis (AGE) responsible for pandemics every ~3 years, and over 200,000 deaths per year, with the majority in children from developing countries. We investigate the incidence of NoV in children hospitalized with AGE from Belém, Pará, Brazil, and also correlated viral RNA levels in their blood and stool with clinical severity. For this purpose, paired stool and serum samples were collected from 445 pediatric patients, ≤9 years between March 2012 and June 2015. Enzyme-linked immunosorbent assay (EIA) was used to detect NoV in stool and reverse transcription quantitative PCR (RT-qPCR) used to quantify NoV RNA levels in sera (RNAemia) and in the positive stool. Positives samples were characterized by the partial ORF1/2 region sequence of viral genome. NoV antigen was detected in 24.3% (108/445) of stool samples, with RNAemia also present in 20.4% (22/108). RNAemia and a high stool viral load (>107 genome copies/gram of faeces) were associated with longer hospitalizations. The prevalent genotypes were GII.4 Sydney_2012 (71.6%-58/81) and New Orleans_2009 (6.2%-5/81) variants. Eight other genotypes belonging to GII were detected and four of them were recombinant strains. All sera were characterized as GII.4 and shared 100% similarity with their stool. The results suggest that the dissemination of NoV to the blood stream is not uncommon and may be related to increased faecal viral loads and disease severity.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31554/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALtulio2_fumian_etal_IOC_2018.pdftulio2_fumian_etal_IOC_2018.pdfapplication/pdf1861226https://www.arca.fiocruz.br/bitstream/icict/31554/2/tulio2_fumian_etal_IOC_2018.pdfe03edc36e190e9befe48af498bed17bfMD52TEXTtulio2_fumian_etal_IOC_2018.pdf.txttulio2_fumian_etal_IOC_2018.pdf.txtExtracted texttext/plain46077https://www.arca.fiocruz.br/bitstream/icict/31554/3/tulio2_fumian_etal_IOC_2018.pdf.txt7d1b599a926d2f2b9b12c4da900d2361MD53icict/315542022-06-24 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| dc.title.pt_BR.fl_str_mv |
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis |
| title |
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis |
| spellingShingle |
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis Reymão, Tammy Kathlyn Amaral RNA de Norovírus Soro Carga viral fecal Crianças Análise molecular Norovirus RNA Fecal viral load Serum Children Molecular analysis |
| title_short |
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis |
| title_full |
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis |
| title_fullStr |
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis |
| title_full_unstemmed |
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis |
| title_sort |
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis |
| author |
Reymão, Tammy Kathlyn Amaral |
| author_facet |
Reymão, Tammy Kathlyn Amaral Fumian, Tulio Machado Justino, Maria Cleonice Aguiar Hernandez, Juliana Merces Bandeira, Renato Silva Lucena, Maria Silvia Sousa Teixeira, Dielle Monteiro Farias, Fredison Pinheiro Silva, Luciana Damascena Linhares, Alexandre Costa Gabbay, Yvone Benchimol |
| author_role |
author |
| author2 |
Fumian, Tulio Machado Justino, Maria Cleonice Aguiar Hernandez, Juliana Merces Bandeira, Renato Silva Lucena, Maria Silvia Sousa Teixeira, Dielle Monteiro Farias, Fredison Pinheiro Silva, Luciana Damascena Linhares, Alexandre Costa Gabbay, Yvone Benchimol |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Reymão, Tammy Kathlyn Amaral Fumian, Tulio Machado Justino, Maria Cleonice Aguiar Hernandez, Juliana Merces Bandeira, Renato Silva Lucena, Maria Silvia Sousa Teixeira, Dielle Monteiro Farias, Fredison Pinheiro Silva, Luciana Damascena Linhares, Alexandre Costa Gabbay, Yvone Benchimol |
| dc.subject.other.pt_BR.fl_str_mv |
RNA de Norovírus Soro Carga viral fecal Crianças Análise molecular |
| topic |
RNA de Norovírus Soro Carga viral fecal Crianças Análise molecular Norovirus RNA Fecal viral load Serum Children Molecular analysis |
| dc.subject.en.pt_BR.fl_str_mv |
Norovirus RNA Fecal viral load Serum Children Molecular analysis |
| description |
Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil. |
| publishDate |
2018 |
| dc.date.issued.fl_str_mv |
2018 |
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2019-02-12T11:12:19Z |
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2019-02-12T11:12:19Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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REYMÃO, Tammy Kathlyn Amaral; et al. Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis. Plos One, v.13, n.7, e0199763, 14p, July 2018. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/31554 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0199763 |
| identifier_str_mv |
REYMÃO, Tammy Kathlyn Amaral; et al. Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis. Plos One, v.13, n.7, e0199763, 14p, July 2018. 1932-6203 10.1371/journal.pone.0199763 |
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https://www.arca.fiocruz.br/handle/icict/31554 |
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eng |
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eng |
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Public Library of Science |
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Public Library of Science |
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