Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants

Detalhes bibliográficos
Autor(a) principal: Farias, Beatriz Oliveira
Data de Publicação: 2022
Outros Autores: Bianco, Kayo, Nascimento, Ana Paula Alves, Gonçalves de Brito, Andressa Silva, Moreira, Thais Costa, Clementino, Maysa Mandetta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51850
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.
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spelling Farias, Beatriz OliveiraBianco, KayoNascimento, Ana Paula AlvesGonçalves de Brito, Andressa SilvaMoreira, Thais CostaClementino, Maysa Mandetta2022-03-24T12:04:40Z2022-03-24T12:04:40Z2022FARIAS, Beatriz Oliveira et al. Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants. Microbial Drug Resistance, v. 0, n. 0, p. 1-9, 2022.1931-8448https://www.arca.fiocruz.br/handle/icict/5185010.1089/mdr.2021.0254engGenomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.The emergence of vancomycin-resistant Enterococcus faecium (Efm) harboring vanA gene and multidrug-resistant determinants is a relevant public health concern. It is an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment plants (WWTPs). Our study addresses a genomic investigation of vanA-carrying Efm from WWTPs in Brazil. Samples from five WWTPs supplied with sewage from different sources were evaluated. Here we present whole-genome sequencing of eight vanA-Efm isolates performed on Illumina MiSeq platform. All these isolates presented multidrug-resistant profile, and five strains were from treated wastewater. Multiple antimicrobial resistance genes (ARGs) were found, such as aph(3′)-IIIa, ant(6′)-Ia, erm(B), and msrC, some of them being allocated in plasmids. The virulence profile was predominantly constituted by efaAfm and acm genes and all isolates, except for one, were predicted as human pathogens. Multilocus sequence typing analysis revealed a new allele and five different STs, three previously described (ST32, ST168, and ST253) and two novel ones (ST1893 and ST1894). Six strains belonged to CC17, often associated with hospital outbreaks. As far as our knowledge, no genomic studies of vanA-Efm recovered from WWTPs revealed isolates belonging to CC17 in Brazil. Therefore, our findings point to the environmental spread of Efm carrying multiple ARGs.Vancomycin-resistant enterococci (VRE)Antibiotic resistanceWhole-genome sequencingWastewaterEnterococos Resistentes à VancomicinaResistência Microbiana a MedicamentosSequenciamento Completo do GenomaÁguas Residuáriasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83102https://www.arca.fiocruz.br/bitstream/icict/51850/1/license.txt3e59f4bb248f3215f9b063e1264ea7aeMD51ORIGINALmdr.2021.0254.pdfmdr.2021.0254.pdfapplication/pdf595114https://www.arca.fiocruz.br/bitstream/icict/51850/2/mdr.2021.0254.pdf698a5ecfbd42896606ede4a1f0503d0eMD52icict/518502022-06-03 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dc.title.pt_BR.fl_str_mv Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
title Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
spellingShingle Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
Farias, Beatriz Oliveira
Vancomycin-resistant enterococci (VRE)
Antibiotic resistance
Whole-genome sequencing
Wastewater
Enterococos Resistentes à Vancomicina
Resistência Microbiana a Medicamentos
Sequenciamento Completo do Genoma
Águas Residuárias
title_short Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
title_full Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
title_fullStr Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
title_full_unstemmed Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
title_sort Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
author Farias, Beatriz Oliveira
author_facet Farias, Beatriz Oliveira
Bianco, Kayo
Nascimento, Ana Paula Alves
Gonçalves de Brito, Andressa Silva
Moreira, Thais Costa
Clementino, Maysa Mandetta
author_role author
author2 Bianco, Kayo
Nascimento, Ana Paula Alves
Gonçalves de Brito, Andressa Silva
Moreira, Thais Costa
Clementino, Maysa Mandetta
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Farias, Beatriz Oliveira
Bianco, Kayo
Nascimento, Ana Paula Alves
Gonçalves de Brito, Andressa Silva
Moreira, Thais Costa
Clementino, Maysa Mandetta
dc.subject.en.pt_BR.fl_str_mv Vancomycin-resistant enterococci (VRE)
Antibiotic resistance
Whole-genome sequencing
Wastewater
topic Vancomycin-resistant enterococci (VRE)
Antibiotic resistance
Whole-genome sequencing
Wastewater
Enterococos Resistentes à Vancomicina
Resistência Microbiana a Medicamentos
Sequenciamento Completo do Genoma
Águas Residuárias
dc.subject.decs.pt_BR.fl_str_mv Enterococos Resistentes à Vancomicina
Resistência Microbiana a Medicamentos
Sequenciamento Completo do Genoma
Águas Residuárias
description Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-03-24T12:04:40Z
dc.date.available.fl_str_mv 2022-03-24T12:04:40Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIAS, Beatriz Oliveira et al. Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants. Microbial Drug Resistance, v. 0, n. 0, p. 1-9, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51850
dc.identifier.issn.pt_BR.fl_str_mv 1931-8448
dc.identifier.doi.none.fl_str_mv 10.1089/mdr.2021.0254
identifier_str_mv FARIAS, Beatriz Oliveira et al. Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants. Microbial Drug Resistance, v. 0, n. 0, p. 1-9, 2022.
1931-8448
10.1089/mdr.2021.0254
url https://www.arca.fiocruz.br/handle/icict/51850
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51850/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51850/2/mdr.2021.0254.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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