Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters

Detalhes bibliográficos
Autor(a) principal: Fiuza, Jacqueline Araújo
Data de Publicação: 2016
Outros Autores: Dey, Ranadhir, Davenport, Dwann, Abdeladhim, Maha, Meneses, Claudio, Oliveira, Fabiano, Kamhawi, Shaden, Valenzuela, Jesus G., Gannavaram, Sreenivas, Nakhasi, Hira L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14885
Resumo: US Food and Drug Administration. Center for Biologics Evaluation and Research. Laboratory of Emerging Pathogens. Silver Spring, Maryland, United States of America/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil
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spelling Fiuza, Jacqueline AraújoDey, RanadhirDavenport, DwannAbdeladhim, MahaMeneses, ClaudioOliveira, FabianoKamhawi, ShadenValenzuela, Jesus G.Gannavaram, SreenivasNakhasi, Hira L.2016-07-14T14:12:55Z2016-07-14T14:12:55Z2016FIUZA, Jacqueline Araújo et al. Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters. PLoS Negl Trop Dis., vol. 10, n. 1, p. e0004322, 20161935-2735https://www.arca.fiocruz.br/handle/icict/1488510.1371/journal.pntd.0004322engPublic Library of ScienceIntradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamstersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUS Food and Drug Administration. Center for Biologics Evaluation and Research. Laboratory of Emerging Pathogens. Silver Spring, Maryland, United States of America/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrasilUS Food and Drug Administration. Center for Biologics Evaluation and Research. Laboratory of Emerging Pathogens. Silver Spring, Maryland, United States of AmericaUS Food and Drug Administration. Center for Biologics Evaluation and Research. Laboratory of Emerging Pathogens. Silver Spring, Maryland, United States of AmericaNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, Maryland, United States of AmericaNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, Maryland, United States of AmericaNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, Maryland, United States of AmericaNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, Maryland, United States of AmericaNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, Maryland, United States of AmericaUS Food and Drug Administration. Center for Biologics Evaluation and Research. Laboratory of Emerging Pathogens. Silver Spring, Maryland, United States of AmericaUS Food and Drug Administration. Center for Biologics Evaluation and Research. Laboratory of Emerging Pathogens. Silver Spring, Maryland, United States of AmericaBACKGROUND: Visceral leishmaniasis (VL) is a neglected tropical disease and is fatal if untreated. There is no vaccine available against leishmaniasis. The majority of patients with cutaneous leishmaniasis (CL) or VL develop a long-term protective immunity after cure from infection, which indicates that development of an effective vaccine against leishmaniasis is possible. Such protection may also be achieved by immunization with live attenuated parasites that do not cause disease. We have previously reported a protective response in mice, hamsters and dogs with Leishmania donovani centrin gene knock-out parasites (LdCen-/-), a live attenuated parasite with a cell division specific centrin1 gene deletion. In this study we have explored the effects of salivary protein LJM19 as an adjuvant and intradermal (ID) route of immunization on the efficacy of LdCen-/- parasites as a vaccine against virulent L. donovani. METHODOLOGY/PRINCIPAL FINDINGS: To explore the potential of a combination of LdCen-/- parasites and salivary protein LJM19 as vaccine antigens, LdCen-/- ID immunization followed by ID challenge with virulent L. donovani were performed in hamsters in a 9-month follow up study. We determined parasite burden (serial dilution), antibody production (ELISA) and cytokine expression (qPCR) in these animals. Compared to controls, animals immunized with LdCen-/- + LJM19 induced a strong antibody response, a reduction in spleen and liver parasite burden and a higher expression of pro-inflammatory cytokines after immunization and one month post-challenge. Additionally, a low parasite load in lymph nodes, spleen and liver, and a non-inflamed spleen was observed in immunized animals 9 months after the challenge infection. CONCLUSIONS: Our results demonstrate that an ID vaccination using LdCen-/-parasites in combination with sand fly salivary protein LJM19 has the capability to confer long lasting protection against visceral leishmaniasis that is comparable to intravenous or intracardial immunization.CricetinaeLeishmania donovani/geneticsLeishmaniasis, Visceral/prevention & controlProtozoan Proteins/metabolismPsychodidae/metabolismSalivary Proteins and Peptides/immunologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14885/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_ Fiuza_Jacqueline_Intradermal_CPqRR_2016.pdfve_ 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dc.title.pt_BR.fl_str_mv Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters
title Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters
spellingShingle Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters
Fiuza, Jacqueline Araújo
Cricetinae
Leishmania donovani/genetics
Leishmaniasis, Visceral/prevention & control
Protozoan Proteins/metabolism
Psychodidae/metabolism
Salivary Proteins and Peptides/immunology
title_short Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters
title_full Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters
title_fullStr Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters
title_full_unstemmed Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters
title_sort Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters
author Fiuza, Jacqueline Araújo
author_facet Fiuza, Jacqueline Araújo
Dey, Ranadhir
Davenport, Dwann
Abdeladhim, Maha
Meneses, Claudio
Oliveira, Fabiano
Kamhawi, Shaden
Valenzuela, Jesus G.
Gannavaram, Sreenivas
Nakhasi, Hira L.
author_role author
author2 Dey, Ranadhir
Davenport, Dwann
Abdeladhim, Maha
Meneses, Claudio
Oliveira, Fabiano
Kamhawi, Shaden
Valenzuela, Jesus G.
Gannavaram, Sreenivas
Nakhasi, Hira L.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fiuza, Jacqueline Araújo
Dey, Ranadhir
Davenport, Dwann
Abdeladhim, Maha
Meneses, Claudio
Oliveira, Fabiano
Kamhawi, Shaden
Valenzuela, Jesus G.
Gannavaram, Sreenivas
Nakhasi, Hira L.
dc.subject.en.pt_BR.fl_str_mv Cricetinae
Leishmania donovani/genetics
Leishmaniasis, Visceral/prevention & control
Protozoan Proteins/metabolism
Psychodidae/metabolism
Salivary Proteins and Peptides/immunology
topic Cricetinae
Leishmania donovani/genetics
Leishmaniasis, Visceral/prevention & control
Protozoan Proteins/metabolism
Psychodidae/metabolism
Salivary Proteins and Peptides/immunology
description US Food and Drug Administration. Center for Biologics Evaluation and Research. Laboratory of Emerging Pathogens. Silver Spring, Maryland, United States of America/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-07-14T14:12:55Z
dc.date.available.fl_str_mv 2016-07-14T14:12:55Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FIUZA, Jacqueline Araújo et al. Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters. PLoS Negl Trop Dis., vol. 10, n. 1, p. e0004322, 2016
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14885
dc.identifier.issn.pt_BR.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0004322
identifier_str_mv FIUZA, Jacqueline Araújo et al. Intradermal Immunization of Leishmania donovani Centrin Knock-Out Parasites in Combination with Salivary Protein LJM19 from Sand Fly Vector Induces a Durable Protective Immune Response in Hamsters. PLoS Negl Trop Dis., vol. 10, n. 1, p. e0004322, 2016
1935-2735
10.1371/journal.pntd.0004322
url https://www.arca.fiocruz.br/handle/icict/14885
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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