Genetic and biological characterisation of Zika virus isolates from different brazilian regions
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35589 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. |
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Strottmann, Daisy MariaZanluca, CamilaMosimann, Ana Luiza PamplonaKoishi, Andrea CristineAuwerter, Nathalia CavalheiroFaoro, HelissonCataneo, Allan Henrique DepieriKuczera, DiogoWowk, Pryscilla FaniniBordignon, JulianoSantos, Claudia Nunes Duarte dos2019-09-13T19:13:42Z2019-09-13T19:13:42Z2019STROTTMANN, Daisy Maria et al. Genetic and biological characterisation of Zika virus isolates from different Brazilian regions. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-11, 2019.1678-8060https://www.arca.fiocruz.br/handle/icict/3558910.1590/0074-027601901501678-8060porFundação Oswaldo Cruz. Instituto Oswaldo CruzFlavivirusGenoma ViralZika VirusGenetic MarkersWhole Genome SequencingVirus ActivationVirus ZikaSecuenciación Completa del GenomaActivación ViralZika VirusMarcadores GenéticosSequenciamento Completo do GenomaAtivação ViralGenetic and biological characterisation of Zika virus isolates from different brazilian regionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 brazilian epidemics. As methods for this study, the ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. As findings, are eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the ellestablished ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. As main conclusions had that Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/35589/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1678-8060-mioc-114.pdf1678-8060-mioc-114.pdfapplication/pdf3706408https://www.arca.fiocruz.br/bitstream/icict/35589/2/1678-8060-mioc-114.pdf5e14ddf11ac66c68ccd9bf9acf46ec13MD52TEXT1678-8060-mioc-114.pdf.txt1678-8060-mioc-114.pdf.txtExtracted texttext/plain52845https://www.arca.fiocruz.br/bitstream/icict/35589/3/1678-8060-mioc-114.pdf.txt2f3e80678d3040451570647fca6f2d28MD53icict/355892019-09-14 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dc.title.pt_BR.fl_str_mv |
Genetic and biological characterisation of Zika virus isolates from different brazilian regions |
title |
Genetic and biological characterisation of Zika virus isolates from different brazilian regions |
spellingShingle |
Genetic and biological characterisation of Zika virus isolates from different brazilian regions Strottmann, Daisy Maria Flavivirus Genoma Viral Zika Virus Genetic Markers Whole Genome Sequencing Virus Activation Virus Zika Secuenciación Completa del Genoma Activación Viral Zika Virus Marcadores Genéticos Sequenciamento Completo do Genoma Ativação Viral |
title_short |
Genetic and biological characterisation of Zika virus isolates from different brazilian regions |
title_full |
Genetic and biological characterisation of Zika virus isolates from different brazilian regions |
title_fullStr |
Genetic and biological characterisation of Zika virus isolates from different brazilian regions |
title_full_unstemmed |
Genetic and biological characterisation of Zika virus isolates from different brazilian regions |
title_sort |
Genetic and biological characterisation of Zika virus isolates from different brazilian regions |
author |
Strottmann, Daisy Maria |
author_facet |
Strottmann, Daisy Maria Zanluca, Camila Mosimann, Ana Luiza Pamplona Koishi, Andrea Cristine Auwerter, Nathalia Cavalheiro Faoro, Helisson Cataneo, Allan Henrique Depieri Kuczera, Diogo Wowk, Pryscilla Fanini Bordignon, Juliano Santos, Claudia Nunes Duarte dos |
author_role |
author |
author2 |
Zanluca, Camila Mosimann, Ana Luiza Pamplona Koishi, Andrea Cristine Auwerter, Nathalia Cavalheiro Faoro, Helisson Cataneo, Allan Henrique Depieri Kuczera, Diogo Wowk, Pryscilla Fanini Bordignon, Juliano Santos, Claudia Nunes Duarte dos |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Strottmann, Daisy Maria Zanluca, Camila Mosimann, Ana Luiza Pamplona Koishi, Andrea Cristine Auwerter, Nathalia Cavalheiro Faoro, Helisson Cataneo, Allan Henrique Depieri Kuczera, Diogo Wowk, Pryscilla Fanini Bordignon, Juliano Santos, Claudia Nunes Duarte dos |
dc.subject.other.pt_BR.fl_str_mv |
Flavivirus Genoma Viral |
topic |
Flavivirus Genoma Viral Zika Virus Genetic Markers Whole Genome Sequencing Virus Activation Virus Zika Secuenciación Completa del Genoma Activación Viral Zika Virus Marcadores Genéticos Sequenciamento Completo do Genoma Ativação Viral |
dc.subject.en.pt_BR.fl_str_mv |
Zika Virus Genetic Markers Whole Genome Sequencing Virus Activation |
dc.subject.es.pt_BR.fl_str_mv |
Virus Zika Secuenciación Completa del Genoma Activación Viral |
dc.subject.decs.pt_BR.fl_str_mv |
Zika Virus Marcadores Genéticos Sequenciamento Completo do Genoma Ativação Viral |
description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-09-13T19:13:42Z |
dc.date.available.fl_str_mv |
2019-09-13T19:13:42Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
STROTTMANN, Daisy Maria et al. Genetic and biological characterisation of Zika virus isolates from different Brazilian regions. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-11, 2019. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/35589 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-8060 |
dc.identifier.doi.none.fl_str_mv |
10.1590/0074-02760190150 |
dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
identifier_str_mv |
STROTTMANN, Daisy Maria et al. Genetic and biological characterisation of Zika virus isolates from different Brazilian regions. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-11, 2019. 1678-8060 10.1590/0074-02760190150 |
url |
https://www.arca.fiocruz.br/handle/icict/35589 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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