Genetic and biological characterisation of Zika virus isolates from different brazilian regions

Detalhes bibliográficos
Autor(a) principal: Strottmann, Daisy Maria
Data de Publicação: 2019
Outros Autores: Zanluca, Camila, Mosimann, Ana Luiza Pamplona, Koishi, Andrea Cristine, Auwerter, Nathalia Cavalheiro, Faoro, Helisson, Cataneo, Allan Henrique Depieri, Kuczera, Diogo, Wowk, Pryscilla Fanini, Bordignon, Juliano, Santos, Claudia Nunes Duarte dos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35589
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
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spelling Strottmann, Daisy MariaZanluca, CamilaMosimann, Ana Luiza PamplonaKoishi, Andrea CristineAuwerter, Nathalia CavalheiroFaoro, HelissonCataneo, Allan Henrique DepieriKuczera, DiogoWowk, Pryscilla FaniniBordignon, JulianoSantos, Claudia Nunes Duarte dos2019-09-13T19:13:42Z2019-09-13T19:13:42Z2019STROTTMANN, Daisy Maria et al. Genetic and biological characterisation of Zika virus isolates from different Brazilian regions. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-11, 2019.1678-8060https://www.arca.fiocruz.br/handle/icict/3558910.1590/0074-027601901501678-8060porFundação Oswaldo Cruz. Instituto Oswaldo CruzFlavivirusGenoma ViralZika VirusGenetic MarkersWhole Genome SequencingVirus ActivationVirus ZikaSecuenciación Completa del GenomaActivación ViralZika VirusMarcadores GenéticosSequenciamento Completo do GenomaAtivação ViralGenetic and biological characterisation of Zika virus isolates from different brazilian regionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 brazilian epidemics. As methods for this study, the ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. As findings, are eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the ellestablished ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. As main conclusions had that Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/35589/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1678-8060-mioc-114.pdf1678-8060-mioc-114.pdfapplication/pdf3706408https://www.arca.fiocruz.br/bitstream/icict/35589/2/1678-8060-mioc-114.pdf5e14ddf11ac66c68ccd9bf9acf46ec13MD52TEXT1678-8060-mioc-114.pdf.txt1678-8060-mioc-114.pdf.txtExtracted texttext/plain52845https://www.arca.fiocruz.br/bitstream/icict/35589/3/1678-8060-mioc-114.pdf.txt2f3e80678d3040451570647fca6f2d28MD53icict/355892019-09-14 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dc.title.pt_BR.fl_str_mv Genetic and biological characterisation of Zika virus isolates from different brazilian regions
title Genetic and biological characterisation of Zika virus isolates from different brazilian regions
spellingShingle Genetic and biological characterisation of Zika virus isolates from different brazilian regions
Strottmann, Daisy Maria
Flavivirus
Genoma Viral
Zika Virus
Genetic Markers
Whole Genome Sequencing
Virus Activation
Virus Zika
Secuenciación Completa del Genoma
Activación Viral
Zika Virus
Marcadores Genéticos
Sequenciamento Completo do Genoma
Ativação Viral
title_short Genetic and biological characterisation of Zika virus isolates from different brazilian regions
title_full Genetic and biological characterisation of Zika virus isolates from different brazilian regions
title_fullStr Genetic and biological characterisation of Zika virus isolates from different brazilian regions
title_full_unstemmed Genetic and biological characterisation of Zika virus isolates from different brazilian regions
title_sort Genetic and biological characterisation of Zika virus isolates from different brazilian regions
author Strottmann, Daisy Maria
author_facet Strottmann, Daisy Maria
Zanluca, Camila
Mosimann, Ana Luiza Pamplona
Koishi, Andrea Cristine
Auwerter, Nathalia Cavalheiro
Faoro, Helisson
Cataneo, Allan Henrique Depieri
Kuczera, Diogo
Wowk, Pryscilla Fanini
Bordignon, Juliano
Santos, Claudia Nunes Duarte dos
author_role author
author2 Zanluca, Camila
Mosimann, Ana Luiza Pamplona
Koishi, Andrea Cristine
Auwerter, Nathalia Cavalheiro
Faoro, Helisson
Cataneo, Allan Henrique Depieri
Kuczera, Diogo
Wowk, Pryscilla Fanini
Bordignon, Juliano
Santos, Claudia Nunes Duarte dos
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Strottmann, Daisy Maria
Zanluca, Camila
Mosimann, Ana Luiza Pamplona
Koishi, Andrea Cristine
Auwerter, Nathalia Cavalheiro
Faoro, Helisson
Cataneo, Allan Henrique Depieri
Kuczera, Diogo
Wowk, Pryscilla Fanini
Bordignon, Juliano
Santos, Claudia Nunes Duarte dos
dc.subject.other.pt_BR.fl_str_mv Flavivirus
Genoma Viral
topic Flavivirus
Genoma Viral
Zika Virus
Genetic Markers
Whole Genome Sequencing
Virus Activation
Virus Zika
Secuenciación Completa del Genoma
Activación Viral
Zika Virus
Marcadores Genéticos
Sequenciamento Completo do Genoma
Ativação Viral
dc.subject.en.pt_BR.fl_str_mv Zika Virus
Genetic Markers
Whole Genome Sequencing
Virus Activation
dc.subject.es.pt_BR.fl_str_mv Virus Zika
Secuenciación Completa del Genoma
Activación Viral
dc.subject.decs.pt_BR.fl_str_mv Zika Virus
Marcadores Genéticos
Sequenciamento Completo do Genoma
Ativação Viral
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-13T19:13:42Z
dc.date.available.fl_str_mv 2019-09-13T19:13:42Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv STROTTMANN, Daisy Maria et al. Genetic and biological characterisation of Zika virus isolates from different Brazilian regions. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-11, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35589
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760190150
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv STROTTMANN, Daisy Maria et al. Genetic and biological characterisation of Zika virus isolates from different Brazilian regions. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-11, 2019.
1678-8060
10.1590/0074-02760190150
url https://www.arca.fiocruz.br/handle/icict/35589
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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