Genetic and biological characterisation of Zika virus isolates from different Brazilian regions

Detalhes bibliográficos
Autor(a) principal: Strottmann,Daisy Maria
Data de Publicação: 2019
Outros Autores: Zanluca,Camila, Mosimann,Ana Luiza Pamplona, Koishi,Andrea C, Auwerter,Nathalia Cavalheiro, Faoro,Helisson, Cataneo,Allan Henrique Depieri, Kuczera,Diogo, Wowk,Pryscilla Fanini, Bordignon,Juliano, Duarte dos Santos,Claudia Nunes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100345
Resumo: BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.
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spelling Genetic and biological characterisation of Zika virus isolates from different Brazilian regionsZika virusFlavivirusmolecular markersbiological characterisation BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.Instituto Oswaldo Cruz, Ministério da Saúde2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100345Memórias do Instituto Oswaldo Cruz v.114 2019reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760190150info:eu-repo/semantics/openAccessStrottmann,Daisy MariaZanluca,CamilaMosimann,Ana Luiza PamplonaKoishi,Andrea CAuwerter,Nathalia CavalheiroFaoro,HelissonCataneo,Allan Henrique DepieriKuczera,DiogoWowk,Pryscilla FaniniBordignon,JulianoDuarte dos Santos,Claudia Nuneseng2020-04-25T17:53:00Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:22:37.944Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
title Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
spellingShingle Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
Strottmann,Daisy Maria
Zika virus
Flavivirus
molecular markers
biological characterisation
title_short Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
title_full Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
title_fullStr Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
title_full_unstemmed Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
title_sort Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
author Strottmann,Daisy Maria
author_facet Strottmann,Daisy Maria
Zanluca,Camila
Mosimann,Ana Luiza Pamplona
Koishi,Andrea C
Auwerter,Nathalia Cavalheiro
Faoro,Helisson
Cataneo,Allan Henrique Depieri
Kuczera,Diogo
Wowk,Pryscilla Fanini
Bordignon,Juliano
Duarte dos Santos,Claudia Nunes
author_role author
author2 Zanluca,Camila
Mosimann,Ana Luiza Pamplona
Koishi,Andrea C
Auwerter,Nathalia Cavalheiro
Faoro,Helisson
Cataneo,Allan Henrique Depieri
Kuczera,Diogo
Wowk,Pryscilla Fanini
Bordignon,Juliano
Duarte dos Santos,Claudia Nunes
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Strottmann,Daisy Maria
Zanluca,Camila
Mosimann,Ana Luiza Pamplona
Koishi,Andrea C
Auwerter,Nathalia Cavalheiro
Faoro,Helisson
Cataneo,Allan Henrique Depieri
Kuczera,Diogo
Wowk,Pryscilla Fanini
Bordignon,Juliano
Duarte dos Santos,Claudia Nunes
dc.subject.por.fl_str_mv Zika virus
Flavivirus
molecular markers
biological characterisation
topic Zika virus
Flavivirus
molecular markers
biological characterisation
dc.description.none.fl_txt_mv BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.
description BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100345
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100345
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-02760190150
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.114 2019
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
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repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
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