Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism

Detalhes bibliográficos
Autor(a) principal: Caldeira, Roberta Lima
Data de Publicação: 2003
Outros Autores: Carvalho, Omar dos Santos, Mendonça, Cristiane Lafetá Furtado de, Teixeira, Carlos Graeff, Silva, Márcia C. F., Ben, Renata, Maurer, Rafael, Lima, Walter S., Lenzi, Henrique Leonel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S0074-02762003000800011
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3784
Resumo: Acknowledgements to Dr Kentaro Yoshimura, Department of Parasitology, Medical School of University of Akita, Japan, for providing us with Angiostrongylus cantonensis; Joziana Barçante, Department of Parasitology of Federal University of Minas Gerais, Brazil, for maintaining the life cycle of A. vasorum; Ester Maria Mota, Department of Pathology, Oswaldo Cruz Institute-Fiocruz, Rio de Janeiro, Brazil, for being in charge of A. costaricensis life cycle.
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spelling Caldeira, Roberta LimaCarvalho, Omar dos SantosMendonça, Cristiane Lafetá Furtado deTeixeira, Carlos GraeffSilva, Márcia C. F.Ben, RenataMaurer, RafaelLima, Walter S.Lenzi, Henrique Leonel2012-02-28T16:14:04Z2012-02-28T16:14:04Z2003CALDEIRA, Roberta L. et al. Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction-restriction fragment length polymorphism. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 8, p. 1039-1043, 2003.0074-0276https://www.arca.fiocruz.br/handle/icict/378410.1590/S0074-02762003000800011Acknowledgements to Dr Kentaro Yoshimura, Department of Parasitology, Medical School of University of Akita, Japan, for providing us with Angiostrongylus cantonensis; Joziana Barçante, Department of Parasitology of Federal University of Minas Gerais, Brazil, for maintaining the life cycle of A. vasorum; Ester Maria Mota, Department of Pathology, Oswaldo Cruz Institute-Fiocruz, Rio de Janeiro, Brazil, for being in charge of A. costaricensis life cycle.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, MG, BrasilPontifícia Universidade Católica do Rio Grande do Sul. Laboratório de Parasitologia Molecular e Biologia de Parasitas. Porto Alegre, RS, BrasilPontifícia Universidade Católica do Rio Grande do Sul. Laboratório de Parasitologia Molecular e Biologia de Parasitas. Porto Alegre, RS, BrasilPontifícia Universidade Católica do Rio Grande do Sul. Laboratório de Parasitologia Molecular e Biologia de Parasitas. Porto Alegre, RS, BrasilPontifícia Universidade Católica do Rio Grande do Sul. Laboratório de Parasitologia Molecular e Biologia de Parasitas. Porto Alegre, RS, BrasilUniversidade Federal de Minas Gerais.Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Angiostrongylus cantonensis, A. costaricensis, and A. vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection of vertebrate hosts with the third-stage larvae. In order to obtain a diagnostic tool to differentiate larvae and adult worm from the three referred species, polymerase chain reaction-restriction fragment length polymorphism was carried out. The rDNA second internal transcribed spacer (ITS2) and mtDNA cytochrome oxidase I regions were amplified, followed by digestion of fragments with the restriction enzymes RsaI, HapII, AluI, HaeIII, DdeI and ClaI. The enzymes RsaI and ClaI exhibited the most discriminating profiles for the differentiation of the regions COI of mtDNA and ITS2 of rDNA respectively. The methodology using such regions proved to be efficient for the specific differentiation of the three species of Angiostrongylus under study.engFundação Oswaldo Cruz. Instituto Oswaldo CruzMolecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAngiostrongylusPolymerase chain reactionRestriction fragment length polymorphismSecond internal transcribed spacerCytochrome oxidase IAngiostrongylusReação em Cadeia da PolimerasePolimorfismo de Fragmento de RestriçãoCitocromosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMolecular Differentiation of Angiostrongylus costaricensis.pdfMolecular Differentiation of Angiostrongylus costaricensis.pdfapplication/pdf78450https://www.arca.fiocruz.br/bitstream/icict/3784/1/Molecular%20Differentiation%20of%20Angiostrongylus%20costaricensis.pdfb4056563b836313ae38fc27af7870527MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3784/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTMolecular Differentiation of Angiostrongylus costaricensis.pdf.txtMolecular Differentiation of Angiostrongylus costaricensis.pdf.txtExtracted texttext/plain23507https://www.arca.fiocruz.br/bitstream/icict/3784/5/Molecular%20Differentiation%20of%20Angiostrongylus%20costaricensis.pdf.txt6e1ecea4c0e9fb5c7b796d9c893864ecMD55THUMBNAILMolecular Differentiation of Angiostrongylus costaricensis.pdf.jpgMolecular Differentiation of Angiostrongylus costaricensis.pdf.jpgGenerated Thumbnailimage/jpeg2384https://www.arca.fiocruz.br/bitstream/icict/3784/4/Molecular%20Differentiation%20of%20Angiostrongylus%20costaricensis.pdf.jpg52cd3d9621c2d46c52053e5a4c9ce69dMD54icict/37842018-04-06 11:25:52.126oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T14:25:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
title Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
spellingShingle Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
Caldeira, Roberta Lima
Angiostrongylus
Polymerase chain reaction
Restriction fragment length polymorphism
Second internal transcribed spacer
Cytochrome oxidase I
Angiostrongylus
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Citocromos
title_short Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
title_full Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
title_fullStr Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
title_full_unstemmed Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
title_sort Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
author Caldeira, Roberta Lima
author_facet Caldeira, Roberta Lima
Carvalho, Omar dos Santos
Mendonça, Cristiane Lafetá Furtado de
Teixeira, Carlos Graeff
Silva, Márcia C. F.
Ben, Renata
Maurer, Rafael
Lima, Walter S.
Lenzi, Henrique Leonel
author_role author
author2 Carvalho, Omar dos Santos
Mendonça, Cristiane Lafetá Furtado de
Teixeira, Carlos Graeff
Silva, Márcia C. F.
Ben, Renata
Maurer, Rafael
Lima, Walter S.
Lenzi, Henrique Leonel
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Caldeira, Roberta Lima
Carvalho, Omar dos Santos
Mendonça, Cristiane Lafetá Furtado de
Teixeira, Carlos Graeff
Silva, Márcia C. F.
Ben, Renata
Maurer, Rafael
Lima, Walter S.
Lenzi, Henrique Leonel
dc.subject.en.pt_BR.fl_str_mv Angiostrongylus
Polymerase chain reaction
Restriction fragment length polymorphism
Second internal transcribed spacer
Cytochrome oxidase I
topic Angiostrongylus
Polymerase chain reaction
Restriction fragment length polymorphism
Second internal transcribed spacer
Cytochrome oxidase I
Angiostrongylus
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Citocromos
dc.subject.decs.pt_BR.fl_str_mv Angiostrongylus
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Citocromos
description Acknowledgements to Dr Kentaro Yoshimura, Department of Parasitology, Medical School of University of Akita, Japan, for providing us with Angiostrongylus cantonensis; Joziana Barçante, Department of Parasitology of Federal University of Minas Gerais, Brazil, for maintaining the life cycle of A. vasorum; Ester Maria Mota, Department of Pathology, Oswaldo Cruz Institute-Fiocruz, Rio de Janeiro, Brazil, for being in charge of A. costaricensis life cycle.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2012-02-28T16:14:04Z
dc.date.available.fl_str_mv 2012-02-28T16:14:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CALDEIRA, Roberta L. et al. Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction-restriction fragment length polymorphism. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 8, p. 1039-1043, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3784
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762003000800011
identifier_str_mv CALDEIRA, Roberta L. et al. Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction-restriction fragment length polymorphism. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 8, p. 1039-1043, 2003.
0074-0276
10.1590/S0074-02762003000800011
url https://www.arca.fiocruz.br/handle/icict/3784
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
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