In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30338 |
Resumo: | Centro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil. |
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Silva, Simone daPinheiro, Ester NetaAssunção, Laís MedeirosSilva, Efigênia LopesRodrigues, Daniele CarvalhoFerreira, Flávio FreiresMarinho, Vitor Rafael PereiraAstolfi-Filho, Spartaco2018-11-29T15:45:29Z2018-11-29T15:45:29Z2018SILVA, Simone da et al. In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid. Revista Fitos, Rio de Janeiro, v. 12, n. 3, p. 263-268, out. 2018.https://www.arca.fiocruz.br/handle/icict/3033810.17648/2446-4775.2018.6202473-4775Fundação Oswaldo Cruz. Farmanguinhos. Núcleo de Gestão em Biodiversidade e Saúde.In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acidinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCentro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil.Centro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil.Centro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil.Centro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil.Centro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil.Centro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil.Centro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil.Universidade Federal do Amazonas. Centro de Apoio Multidisciplinar. Manaus, AM, Brasil.Psychotria ipecacuanha is a medicinal plant that is native to the Americas with the largest area of occurrence in the State of Mato Grosso in Brazil. It is critically endangered due to overexploitation of natural populations. Due to difficulties in conventional propagation, the aim of this study was to evaluate the effect of different concentrations of the growth regulator indoleacetic acid (IAA) on in vitro propagation of this species. Stem node explants were cultured in Murashige and Skoog medium (MS) without growth regulators (control) and supplemented with four concentrations (0.05, 0.5, 1.5 and 2.0 mg.L-1) of indoleacetic acid (IAA) in semisolid media. After 60 days of cultivation, nodal segments (n=30) grown in Murashige & Skoog (MS) medium supplemented with 0.05 mg.L-1 of IAA produced on average 4.56 nodal segments per explant. The seedlings were successfully acclimatized without detection of any morphological anomalies or variation.Plant BiotechnologyTissue CultureMicropropagationRubiaceaeIpecacinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/30338/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALsimone_et_all.pdfapplication/pdf210912https://www.arca.fiocruz.br/bitstream/icict/30338/2/simone_et_all.pdf6b292fdf165d0ae14d671375dac3cb53MD52TEXTsimone_et_all.pdf.txtsimone_et_all.pdf.txtExtracted texttext/plain18832https://www.arca.fiocruz.br/bitstream/icict/30338/3/simone_et_all.pdf.txt92eed7e8f1597c133e613a7ccf16fb44MD53icict/303382020-03-25 23:06:37.457oai:www.arca.fiocruz.br:icict/30338Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352020-03-26T02:06:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
title |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
spellingShingle |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid Silva, Simone da Plant Biotechnology Tissue Culture Micropropagation Rubiaceae Ipecac |
title_short |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
title_full |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
title_fullStr |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
title_full_unstemmed |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
title_sort |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
author |
Silva, Simone da |
author_facet |
Silva, Simone da Pinheiro, Ester Neta Assunção, Laís Medeiros Silva, Efigênia Lopes Rodrigues, Daniele Carvalho Ferreira, Flávio Freires Marinho, Vitor Rafael Pereira Astolfi-Filho, Spartaco |
author_role |
author |
author2 |
Pinheiro, Ester Neta Assunção, Laís Medeiros Silva, Efigênia Lopes Rodrigues, Daniele Carvalho Ferreira, Flávio Freires Marinho, Vitor Rafael Pereira Astolfi-Filho, Spartaco |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Simone da Pinheiro, Ester Neta Assunção, Laís Medeiros Silva, Efigênia Lopes Rodrigues, Daniele Carvalho Ferreira, Flávio Freires Marinho, Vitor Rafael Pereira Astolfi-Filho, Spartaco |
dc.subject.en.none.fl_str_mv |
Plant Biotechnology Tissue Culture Micropropagation Rubiaceae Ipecac |
topic |
Plant Biotechnology Tissue Culture Micropropagation Rubiaceae Ipecac |
description |
Centro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-11-29T15:45:29Z |
dc.date.available.fl_str_mv |
2018-11-29T15:45:29Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Simone da et al. In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid. Revista Fitos, Rio de Janeiro, v. 12, n. 3, p. 263-268, out. 2018. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/30338 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.17648/2446-4775.2018.620 |
dc.identifier.eissn.pt_BR.fl_str_mv |
2473-4775 |
identifier_str_mv |
SILVA, Simone da et al. In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid. Revista Fitos, Rio de Janeiro, v. 12, n. 3, p. 263-268, out. 2018. 10.17648/2446-4775.2018.620 2473-4775 |
url |
https://www.arca.fiocruz.br/handle/icict/30338 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Farmanguinhos. Núcleo de Gestão em Biodiversidade e Saúde. |
publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Farmanguinhos. Núcleo de Gestão em Biodiversidade e Saúde. |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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