Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae)

• Autor(a) principal: da Silva, Simone
• Data de Publicação: 2018
• Outros Autores: Astolfi Filho, Spartaco
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Fitos
• Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/616
• topic: Ipecac. Root culture. In vitro culture. Plant biotechnology.

Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) is a medicinal plant commonly known as ipecac, which contains two alkaloids of high pharmacological value (emetine and cephaeline) with anti-diarrheal, amebicidal, expectorant and anti-inflammatory properties. The risk of extinction and the difficulty of the conventional cultivation of the species combined with great market demand has generated a need for studies of cultivation methods that allow the economic exploitation of this species. This work presents data on the development of an in vitro root culture protocol for P. ipecacuanha. Leaf, nodal, internodal and root segments were used, which were introduced in culture media containing different concentrations (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg.L-1) of indolbutiric acid. The best treatment for root formation included the use of nodal segments in culture media containing 2.5 and 3.0 mg.L-1 of IBA, which gave rise to 100% root formation at 60 days of culture. Regarding the number of roots per explant, the best result (13:1) was obtained with the use of internodal segments in medium supplemented with 2.5 mg.L-1 IBA and, the best root length was obtained in root segments developed in medium enriched with 1.5 mg.L-1 IBA, with an average increment of 0.87 cm per root.