Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18442 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil. |
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Dias, André AlvesRaze, DominiqueLima, Cristiane Soares deMarques, Maria Angela de MeloDrobecq, HervéDebrie, Anne-SophieGuimarães, Michelle Lopes RibeiroBiet, FranckPessolani, Maria Cristina Vidal2017-04-13T19:01:36Z2017-04-13T19:01:36Z2012DIAS, André Alves; et al. Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence. Mem Inst Oswaldo Cruz, v.107, Suppl. I, p.174-182, 2012.1678-8080https://www.arca.fiocruz.br/handle/icict/18442engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.HanseníaseTuberculoseMicobacteriacolagénioMycobacteriacytoadherencecollagenhistone-like proteinleprosytuberculosisMycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil.Inserm, U 1019. Lille, France / CNRS. UMR 8204 7CNRS. UMR 8525. Lille, France / Institut Pasteur de Lille. Centre for Infection and Immunity of Lille (CIIL). Lille, France / Université Lille Nord de France. Lille, France.Université Lille Nord de France. Lille, France.Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, Colorado, USA.sem afiliaçãoInserm, U 1019. Lille, France / CNRS. UMR 8204 7CNRS. UMR 8525. Lille, France / Institut Pasteur de Lille. Centre for Infection and Immunity of Lille (CIIL). Lille, France / Université Lille Nord de France. Lille, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil.INRA, UR1282, Infectiologie et Santé Publique. Centre de Tours. Nouzilly, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil.When grown in the presence of exogenous collagen I, Mycobacterium bovis BCG was shown to form clumps. Scanning electron microscopy examination of these clumps revealed the presence of collagen fibres cross-linking the bacilli. Since collagen is a major constituent of the eukaryotic extracellular matrices, we assayed BCG cytoadherence in the presence of exogenous collagen I. Collagen increased the interaction of the bacilli with A549 type II pneumocytes or U937 macrophages, suggesting that BCG is able to recruit collagen to facilitate its attachment to host cells. Using an affinity chromatography approach, we have isolated a BCG collagen-binding protein corresponding to the previously described mycobacterial laminin-binding histone-like protein (LBP/Hlp), a highly conserved protein associated with the mycobacterial cell wall. Moreover, Mycobacterium leprae LBP/Hlp, a wellcharacterized adhesin, was also able to bind collagen I. Finally, using recombinant fragments of M. leprae LBP/ Hlp, we mapped the collagen-binding activity within the C-terminal domain of the adhesin. Since this protein was already shown to be involved in the recognition of laminin and heparan sulphate-containing proteoglycans, the present observations reinforce the adhesive activities of LBP/Hlp, which can be therefore considered as a multifaceted mycobacterial adhesin, playing an important role in both leprosy and tuberculosis pathogenesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18442/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALandre2_dias_etal_IOC_2012.pdfandre2_dias_etal_IOC_2012.pdfapplication/pdf1251359https://www.arca.fiocruz.br/bitstream/icict/18442/2/andre2_dias_etal_IOC_2012.pdff13c319a98560585c98eedaaa78dbe15MD52TEXTandre2_dias_etal_IOC_2012.pdf.txtandre2_dias_etal_IOC_2012.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence |
| title |
Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence |
| spellingShingle |
Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence Dias, André Alves Hanseníase Tuberculose Micobacteria colagénio Mycobacteria cytoadherence collagen histone-like protein leprosy tuberculosis |
| title_short |
Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence |
| title_full |
Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence |
| title_fullStr |
Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence |
| title_full_unstemmed |
Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence |
| title_sort |
Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence |
| author |
Dias, André Alves |
| author_facet |
Dias, André Alves Raze, Dominique Lima, Cristiane Soares de Marques, Maria Angela de Melo Drobecq, Hervé Debrie, Anne-Sophie Guimarães, Michelle Lopes Ribeiro Biet, Franck Pessolani, Maria Cristina Vidal |
| author_role |
author |
| author2 |
Raze, Dominique Lima, Cristiane Soares de Marques, Maria Angela de Melo Drobecq, Hervé Debrie, Anne-Sophie Guimarães, Michelle Lopes Ribeiro Biet, Franck Pessolani, Maria Cristina Vidal |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Dias, André Alves Raze, Dominique Lima, Cristiane Soares de Marques, Maria Angela de Melo Drobecq, Hervé Debrie, Anne-Sophie Guimarães, Michelle Lopes Ribeiro Biet, Franck Pessolani, Maria Cristina Vidal |
| dc.subject.other.pt_BR.fl_str_mv |
Hanseníase Tuberculose Micobacteria colagénio |
| topic |
Hanseníase Tuberculose Micobacteria colagénio Mycobacteria cytoadherence collagen histone-like protein leprosy tuberculosis |
| dc.subject.en.pt_BR.fl_str_mv |
Mycobacteria cytoadherence collagen histone-like protein leprosy tuberculosis |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2012 |
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2012 |
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2017-04-13T19:01:36Z |
| dc.date.available.fl_str_mv |
2017-04-13T19:01:36Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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DIAS, André Alves; et al. Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence. Mem Inst Oswaldo Cruz, v.107, Suppl. I, p.174-182, 2012. |
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https://www.arca.fiocruz.br/handle/icict/18442 |
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1678-8080 |
| identifier_str_mv |
DIAS, André Alves; et al. Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence. Mem Inst Oswaldo Cruz, v.107, Suppl. I, p.174-182, 2012. 1678-8080 |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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