Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45983 |
Resumo: | Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil. |
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Henriques, Daniele FreitasNunes, Juliana A.L.Anjos, Maura V.Melo, Juliana M.Rosário, Wallace O.Azevedo, Raimunda S. S.Chiang, Jannifer O.Martins, Lívia C.Santos, Flavia B. dosCasseb, Livia M.N.Vaconcelos, Pedro F. C.Rodrigues, Sueli G.2021-02-08T13:07:16Z2021-02-08T13:07:16Z2020HENRIQUES, Daniele Freitas et al. Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory. Journal of Virological Methods, v. 286, 113976, 5p, Sept. 2020.0166-0934https://www.arca.fiocruz.br/handle/icict/4598310.1016/j.jviromet.2020.113976engElsevierFlavivírusDengueZikaFebre AmarelaELISAIgMTestes comerciaisFlavivirusesDengueZikaYellow feverELISAIgMCommercial testsEvaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratoryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Zika and Dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. Due to Brazil’s wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by National Reference Laboratory for Arboviruses in Brazil and evaluate commercial tests available. We evaluated in house IgM ELISAs for the individual detection of anti-ZIKV, -DENV, and –YFV IgM, against a panel of samples positive for dengue, zika, yellow fever, Rocio, Ilheus, Saint Louis encephalitis, West Nile and chikungunya. We also evaluated two commercial kits for dengue and zika IgM detection recommended by the Brazilian Ministry of Health in 2015. The sensitivity and specificity for the in house ZIKV IgM ELISA was 60.0 % and 88.6 % and for the in house DENV IgM ELISA was 100 % and 82.2 %, respectively. The in house YFV IgM ELISA presented 100 % for both sensitivity and specificity. The Novagnost Zika Virus IgM test presented a sensitivity of 47.3 % and specificity of 85.3 % and the Serion ELISA classic Dengue Virus IgM, 92.8 % and 58.9 %, respectively. Overall, both in house ELISAs for ZIKV and DENV adapted and evaluated here, presented better performances than the commercial kits tested.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45983/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDanielFreitas_FlaviaBSantos_etal_IOC_2020.pdfDanielFreitas_FlaviaBSantos_etal_IOC_2020.pdfapplication/pdf1126764https://www.arca.fiocruz.br/bitstream/icict/45983/2/DanielFreitas_FlaviaBSantos_etal_IOC_2020.pdf17d805077ac3f1bfddf913f9b5aa260fMD52TEXTDanielFreitas_FlaviaBSantos_etal_IOC_2020.pdf.txtDanielFreitas_FlaviaBSantos_etal_IOC_2020.pdf.txtExtracted texttext/plain30833https://www.arca.fiocruz.br/bitstream/icict/45983/3/DanielFreitas_FlaviaBSantos_etal_IOC_2020.pdf.txt983b412322e2e90e4d989327b5ea9787MD53icict/459832021-02-09 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| dc.title.pt_BR.fl_str_mv |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| spellingShingle |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory Henriques, Daniele Freitas Flavivírus Dengue Zika Febre Amarela ELISA IgM Testes comerciais Flaviviruses Dengue Zika Yellow fever ELISA IgM Commercial tests |
| title_short |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title_full |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title_fullStr |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title_full_unstemmed |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title_sort |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| author |
Henriques, Daniele Freitas |
| author_facet |
Henriques, Daniele Freitas Nunes, Juliana A.L. Anjos, Maura V. Melo, Juliana M. Rosário, Wallace O. Azevedo, Raimunda S. S. Chiang, Jannifer O. Martins, Lívia C. Santos, Flavia B. dos Casseb, Livia M.N. Vaconcelos, Pedro F. C. Rodrigues, Sueli G. |
| author_role |
author |
| author2 |
Nunes, Juliana A.L. Anjos, Maura V. Melo, Juliana M. Rosário, Wallace O. Azevedo, Raimunda S. S. Chiang, Jannifer O. Martins, Lívia C. Santos, Flavia B. dos Casseb, Livia M.N. Vaconcelos, Pedro F. C. Rodrigues, Sueli G. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Henriques, Daniele Freitas Nunes, Juliana A.L. Anjos, Maura V. Melo, Juliana M. Rosário, Wallace O. Azevedo, Raimunda S. S. Chiang, Jannifer O. Martins, Lívia C. Santos, Flavia B. dos Casseb, Livia M.N. Vaconcelos, Pedro F. C. Rodrigues, Sueli G. |
| dc.subject.other.pt_BR.fl_str_mv |
Flavivírus Dengue Zika Febre Amarela ELISA IgM Testes comerciais |
| topic |
Flavivírus Dengue Zika Febre Amarela ELISA IgM Testes comerciais Flaviviruses Dengue Zika Yellow fever ELISA IgM Commercial tests |
| dc.subject.en.pt_BR.fl_str_mv |
Flaviviruses Dengue Zika Yellow fever ELISA IgM Commercial tests |
| description |
Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil. |
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2020 |
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2020 |
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2021-02-08T13:07:16Z |
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2021-02-08T13:07:16Z |
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info:eu-repo/semantics/article |
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HENRIQUES, Daniele Freitas et al. Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory. Journal of Virological Methods, v. 286, 113976, 5p, Sept. 2020. |
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https://www.arca.fiocruz.br/handle/icict/45983 |
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0166-0934 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.jviromet.2020.113976 |
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HENRIQUES, Daniele Freitas et al. Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory. Journal of Virological Methods, v. 286, 113976, 5p, Sept. 2020. 0166-0934 10.1016/j.jviromet.2020.113976 |
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