Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4182 |
Resumo: | Zika and Dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. Due to Brazil’s wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by National Reference Laboratory for Arboviruses in Brazil and evaluate commercial tests available. We evaluated in house IgM ELISAs for the individual detection of anti-ZIKV, -DENV, and –YFV IgM, against a panel of samples positive for dengue, zika, yellow fever, Rocio, Ilheus, Saint Louis encephalitis, West Nile and chikungunya. We also evaluated two commercial kits for dengue and zika IgM detection recommended by the Brazilian Ministry of Health in 2015. The sensitivity and specificity for the in house ZIKV IgM ELISA was 60.0 % and 88.6 % and for the in house DENV IgM ELISA was 100 % and 82.2 %, respectively. The in house YFV IgM ELISA presented 100 % for both sensitivity and specificity. The Novagnost Zika Virus IgM test presented a sensitivity of 47.3 % and specificity of 85.3 % and the Serion ELISA classic Dengue Virus IgM, 92.8 % and 58.9 %, respectively. Overall, both in house ELISAs for ZIKV and DENV adapted and evaluated here, presented better performances than the commercial kits tested. |
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Henriques, Daniele FreitasNunes, Juliana A. LAnjos, Maura Viana dosMelo, Juliana MarinhoRosário, Wallace OAzevedo, Raimunda do Socorro da SilvaChiang, Jannifer OliveiraMartins, Lívia CaricioSantos, Flavia B. dosCasseb, Lívia Medeiros NevesVasconcelos, Pedro Fernando da CostaRodrigues, Sueli Guerreiro2020-10-13T17:44:22Z2020-10-13T17:44:22Z2020HENRIQUES, Daniele Freitas et al. Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory. Journal of Virological Methods, v. 286, n. 113976, p. xx, Dec. 2020.0166-0934https://patua.iec.gov.br/handle/iec/418210.1016/j.jviromet.2020.113976Zika and Dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. Due to Brazil’s wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by National Reference Laboratory for Arboviruses in Brazil and evaluate commercial tests available. We evaluated in house IgM ELISAs for the individual detection of anti-ZIKV, -DENV, and –YFV IgM, against a panel of samples positive for dengue, zika, yellow fever, Rocio, Ilheus, Saint Louis encephalitis, West Nile and chikungunya. We also evaluated two commercial kits for dengue and zika IgM detection recommended by the Brazilian Ministry of Health in 2015. The sensitivity and specificity for the in house ZIKV IgM ELISA was 60.0 % and 88.6 % and for the in house DENV IgM ELISA was 100 % and 82.2 %, respectively. The in house YFV IgM ELISA presented 100 % for both sensitivity and specificity. The Novagnost Zika Virus IgM test presented a sensitivity of 47.3 % and specificity of 85.3 % and the Serion ELISA classic Dengue Virus IgM, 92.8 % and 58.9 %, respectively. Overall, both in house ELISAs for ZIKV and DENV adapted and evaluated here, presented better performances than the commercial kits tested.Evandro Chagas Institute/Ministry of Health (IEC/MS) from the Brazilian Government.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Oswaldo Cruz Institute. Viral Immunology Laboratory. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engElsevierEvaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratoryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFlavivirus / isolamento & purificaçãoInfecções por FlavivirusDengueZika VirusFebre AmarelaEnsaio de Imunoadsorção EnzimáticaImunoglobulina MTestes LaboratoriaisVigilância de Produtos Comercializadosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALEvaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory.pdfEvaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory.pdfapplication/pdf1126764https://patua.iec.gov.br/bitstreams/fc5fad85-5564-4208-a5b2-5653303c46e0/download17d805077ac3f1bfddf913f9b5aa260fMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
title |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
spellingShingle |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory Henriques, Daniele Freitas Flavivirus / isolamento & purificação Infecções por Flavivirus Dengue Zika Virus Febre Amarela Ensaio de Imunoadsorção Enzimática Imunoglobulina M Testes Laboratoriais Vigilância de Produtos Comercializados |
title_short |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
title_full |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
title_fullStr |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
title_full_unstemmed |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
title_sort |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
author |
Henriques, Daniele Freitas |
author_facet |
Henriques, Daniele Freitas Nunes, Juliana A. L Anjos, Maura Viana dos Melo, Juliana Marinho Rosário, Wallace O Azevedo, Raimunda do Socorro da Silva Chiang, Jannifer Oliveira Martins, Lívia Caricio Santos, Flavia B. dos Casseb, Lívia Medeiros Neves Vasconcelos, Pedro Fernando da Costa Rodrigues, Sueli Guerreiro |
author_role |
author |
author2 |
Nunes, Juliana A. L Anjos, Maura Viana dos Melo, Juliana Marinho Rosário, Wallace O Azevedo, Raimunda do Socorro da Silva Chiang, Jannifer Oliveira Martins, Lívia Caricio Santos, Flavia B. dos Casseb, Lívia Medeiros Neves Vasconcelos, Pedro Fernando da Costa Rodrigues, Sueli Guerreiro |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Henriques, Daniele Freitas Nunes, Juliana A. L Anjos, Maura Viana dos Melo, Juliana Marinho Rosário, Wallace O Azevedo, Raimunda do Socorro da Silva Chiang, Jannifer Oliveira Martins, Lívia Caricio Santos, Flavia B. dos Casseb, Lívia Medeiros Neves Vasconcelos, Pedro Fernando da Costa Rodrigues, Sueli Guerreiro |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Flavivirus / isolamento & purificação Infecções por Flavivirus Dengue Zika Virus Febre Amarela Ensaio de Imunoadsorção Enzimática Imunoglobulina M Testes Laboratoriais Vigilância de Produtos Comercializados |
topic |
Flavivirus / isolamento & purificação Infecções por Flavivirus Dengue Zika Virus Febre Amarela Ensaio de Imunoadsorção Enzimática Imunoglobulina M Testes Laboratoriais Vigilância de Produtos Comercializados |
description |
Zika and Dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. Due to Brazil’s wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by National Reference Laboratory for Arboviruses in Brazil and evaluate commercial tests available. We evaluated in house IgM ELISAs for the individual detection of anti-ZIKV, -DENV, and –YFV IgM, against a panel of samples positive for dengue, zika, yellow fever, Rocio, Ilheus, Saint Louis encephalitis, West Nile and chikungunya. We also evaluated two commercial kits for dengue and zika IgM detection recommended by the Brazilian Ministry of Health in 2015. The sensitivity and specificity for the in house ZIKV IgM ELISA was 60.0 % and 88.6 % and for the in house DENV IgM ELISA was 100 % and 82.2 %, respectively. The in house YFV IgM ELISA presented 100 % for both sensitivity and specificity. The Novagnost Zika Virus IgM test presented a sensitivity of 47.3 % and specificity of 85.3 % and the Serion ELISA classic Dengue Virus IgM, 92.8 % and 58.9 %, respectively. Overall, both in house ELISAs for ZIKV and DENV adapted and evaluated here, presented better performances than the commercial kits tested. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-10-13T17:44:22Z |
dc.date.available.fl_str_mv |
2020-10-13T17:44:22Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
HENRIQUES, Daniele Freitas et al. Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory. Journal of Virological Methods, v. 286, n. 113976, p. xx, Dec. 2020. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4182 |
dc.identifier.issn.-.fl_str_mv |
0166-0934 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.jviromet.2020.113976 |
identifier_str_mv |
HENRIQUES, Daniele Freitas et al. Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory. Journal of Virological Methods, v. 286, n. 113976, p. xx, Dec. 2020. 0166-0934 10.1016/j.jviromet.2020.113976 |
url |
https://patua.iec.gov.br/handle/iec/4182 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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