Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection

Detalhes bibliográficos
Autor(a) principal: Souza, Raquel Lopes Martins
Data de Publicação: 2006
Outros Autores: Pereira, C. A. J., Martins Filho, Olindo Assis, Coelho, Paulo Marcos Zach, Corrêa Junior, Ary, Corrêa, Deborah Aparecida Negrão
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6568
Resumo: Pronex/CNPq, Fapemig
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spelling Souza, Raquel Lopes MartinsPereira, C. A. J.Martins Filho, Olindo AssisCoelho, Paulo Marcos ZachCorrêa Junior, AryCorrêa, Deborah Aparecida Negrão2013-06-06T12:52:55Z2013-06-06T12:52:55Z2006MARTINS SOUZA, RL et al. Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection. MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 101 (Suppl.I), 2006, pp 185-1920074-0276https://www.arca.fiocruz.br/handle/icict/656810.1590/S0074-02762006000900029Pronex/CNPq, FapemigUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Comissão Interna de Biossegurança. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Esquistossomose - Serviço de Referência de Esquistossomose. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilLectins/carbohydrate binding can be involved in the Schistosoma mansoni recognition and activation of the Biomphalaria hemocytes. Therefore, expression of lectin ligands on Biomphalaria hemocytes would be associated with snail resistance against S. mansoniinfection. To test this hypothesis, circulating hemocytes were isolated from B. glabrataBH (snail strain highy susceptible to S. mansoni), B. tenagophilaCabo Frio (moderate susceptibility), and B. tenagophilaTaim (completely resistant strains), labelled with FITC conjugated lectins (ConA, PNA, SBA, and WGA) and analyzed under fluorescence microscopy. The results demonstrated that although lectin-labelled hemocytes were detected in hemolymph of all snail species tested, circulating hemocytes from both strains ofB. Tenagophila showed a larger number of lectin-labelled cells than B. glabrata. Moreover, most of circulating hemocytes of B. tenagophilawere intensively labelled by lectins PNA-FITC and WGA-FITC, while in B. glabrata small hemocytes were labeled mainly by ConA. Upon S. mansoniinfection, lectin-labelled hemocytes almost disappeared from the hemolymph of Taim and accumulated in B. glabrataBH. The role of lectins/carbohydrate binding in resistance of B.tengophila infection to S. mansoniis still not fully understood, but the data suggest that there may be a correlation to its presence with susceptibility or resistance to the parasite.engDifferential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSchistosoma mansoniBiomphalaria tenagophilasnail susceptibility to trematodelectin labelling of hemocyteshost-parasite interactioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL70.pdf70.pdfapplication/pdf812274https://www.arca.fiocruz.br/bitstream/icict/6568/1/70.pdfa49155d775e3c77975d942102d8ce87aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/6568/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXT70.pdf.txt70.pdf.txtExtracted texttext/plain32675https://www.arca.fiocruz.br/bitstream/icict/6568/5/70.pdf.txt1eb06fc9ca9ff5f1d33ed1fdc65608dcMD55THUMBNAIL70.pdf.jpg70.pdf.jpgGenerated Thumbnailimage/jpeg2234https://www.arca.fiocruz.br/bitstream/icict/6568/4/70.pdf.jpg1b5b9779b6c0e81481de7f36db90d99eMD54icict/65682018-04-25 15:13:01.597oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-25T18:13:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection
title Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection
spellingShingle Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection
Souza, Raquel Lopes Martins
Schistosoma mansoni
Biomphalaria tenagophila
snail susceptibility to trematode
lectin labelling of hemocytes
host-parasite interaction
title_short Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection
title_full Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection
title_fullStr Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection
title_full_unstemmed Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection
title_sort Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection
author Souza, Raquel Lopes Martins
author_facet Souza, Raquel Lopes Martins
Pereira, C. A. J.
Martins Filho, Olindo Assis
Coelho, Paulo Marcos Zach
Corrêa Junior, Ary
Corrêa, Deborah Aparecida Negrão
author_role author
author2 Pereira, C. A. J.
Martins Filho, Olindo Assis
Coelho, Paulo Marcos Zach
Corrêa Junior, Ary
Corrêa, Deborah Aparecida Negrão
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Raquel Lopes Martins
Pereira, C. A. J.
Martins Filho, Olindo Assis
Coelho, Paulo Marcos Zach
Corrêa Junior, Ary
Corrêa, Deborah Aparecida Negrão
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Biomphalaria tenagophila
snail susceptibility to trematode
lectin labelling of hemocytes
host-parasite interaction
topic Schistosoma mansoni
Biomphalaria tenagophila
snail susceptibility to trematode
lectin labelling of hemocytes
host-parasite interaction
description Pronex/CNPq, Fapemig
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2013-06-06T12:52:55Z
dc.date.available.fl_str_mv 2013-06-06T12:52:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv MARTINS SOUZA, RL et al. Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection. MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 101 (Suppl.I), 2006, pp 185-192
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6568
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762006000900029
identifier_str_mv MARTINS SOUZA, RL et al. Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection. MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 101 (Suppl.I), 2006, pp 185-192
0074-0276
10.1590/S0074-02762006000900029
url https://www.arca.fiocruz.br/handle/icict/6568
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