Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection

Detalhes bibliográficos
Autor(a) principal: Oliveira, A. L. D.
Data de Publicação: 2010
Outros Autores: Levada, P. M. [UNESP], Zanotti-Magalhaes, E. M., Magalhaes, L. A., Ribeiro-Paes, J. T. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4238/vol9-4gmr1143
http://hdl.handle.net/11449/6536
Resumo: The relationships between schistosomiasis and its intermediate host, mollusks of the genus Biomphalaria, have been a concern for decades. It is known that the vector mollusk shows different susceptibility against parasite infection, whose occurrence depends on the interaction between the forms of trematode larvae and the host defense cells. These cells are called amebocytes or hemocytes and are responsible for the recognition of foreign bodies and for phagocytosis and cytotoxic reactions. The defense cells mediate the modulation of the resistant and susceptible phenotypes of the mollusk. Two main types of hemocytes are found in the Biomphalaria hemolymph: the granulocytes and the hyalinocytes. We studied the variation in the number (kinetics) of hemocytes for 24 h after exposing the parasite to genetically selected and non-selected strains of Biomphalaria tenagophila, susceptible or not to infection by Schistosoma mansoni. The differences were analyzed referred to the variations in the number of hemocytes in mollusks susceptible or not to infection by S. mansoni. The hemolymph of the selected and non-selected snails was collected, and hemocytes were counted using a Neubauer chamber at six designated periods: 0 h (control, non-exposed individuals), 2 h, 6 h, 12 h, 18 h and, 24 h after parasite exposure. Samples of hemolymph of five selected mollusks and five non-selected mollusks were separately used at each counting time. There was a significant variation in the number of hemocytes between the strains, which indicates that defense cells have different behaviors in resistant and susceptible mollusks.
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spelling Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infectionBiomphalaria tenagophilaSchistosoma mansoniHemocyte behaviorSchistosomiasisBiological controlThe relationships between schistosomiasis and its intermediate host, mollusks of the genus Biomphalaria, have been a concern for decades. It is known that the vector mollusk shows different susceptibility against parasite infection, whose occurrence depends on the interaction between the forms of trematode larvae and the host defense cells. These cells are called amebocytes or hemocytes and are responsible for the recognition of foreign bodies and for phagocytosis and cytotoxic reactions. The defense cells mediate the modulation of the resistant and susceptible phenotypes of the mollusk. Two main types of hemocytes are found in the Biomphalaria hemolymph: the granulocytes and the hyalinocytes. We studied the variation in the number (kinetics) of hemocytes for 24 h after exposing the parasite to genetically selected and non-selected strains of Biomphalaria tenagophila, susceptible or not to infection by Schistosoma mansoni. The differences were analyzed referred to the variations in the number of hemocytes in mollusks susceptible or not to infection by S. mansoni. The hemolymph of the selected and non-selected snails was collected, and hemocytes were counted using a Neubauer chamber at six designated periods: 0 h (control, non-exposed individuals), 2 h, 6 h, 12 h, 18 h and, 24 h after parasite exposure. Samples of hemolymph of five selected mollusks and five non-selected mollusks were separately used at each counting time. There was a significant variation in the number of hemocytes between the strains, which indicates that defense cells have different behaviors in resistant and susceptible mollusks.Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Dept Ciencias Biol, Assis, SP, BrazilUniv São Paulo, IPT, Inst Butantan, Programa Posgrad Interunidades Biotecnol, BR-09500900 São Paulo, BrazilUniv Estadual Campinas, Inst Biol, Dept Parasitol, Campinas, SP, BrazilADR, Dept Saude, Assis, SP, BrazilUniv Paulista, Dept Ciencias Saude, Assis, SP, BrazilUniv Estadual Paulista, Dept Ciencias Biol, Assis, SP, BrazilFunpec-editoraUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)ADRUniv PaulistaOliveira, A. L. D.Levada, P. M. [UNESP]Zanotti-Magalhaes, E. M.Magalhaes, L. A.Ribeiro-Paes, J. T. [UNESP]2014-05-20T13:22:20Z2014-05-20T13:22:20Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2436-2445application/pdfhttp://dx.doi.org/10.4238/vol9-4gmr1143Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 4, p. 2436-2445, 2010.1676-5680http://hdl.handle.net/11449/653610.4238/vol9-4gmr1143WOS:000285718400020WOS000285718400020.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2024-06-13T17:38:41Zoai:repositorio.unesp.br:11449/6536Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:09:45.342098Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection
title Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection
spellingShingle Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection
Oliveira, A. L. D.
Biomphalaria tenagophila
Schistosoma mansoni
Hemocyte behavior
Schistosomiasis
Biological control
title_short Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection
title_full Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection
title_fullStr Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection
title_full_unstemmed Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection
title_sort Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection
author Oliveira, A. L. D.
author_facet Oliveira, A. L. D.
Levada, P. M. [UNESP]
Zanotti-Magalhaes, E. M.
Magalhaes, L. A.
Ribeiro-Paes, J. T. [UNESP]
author_role author
author2 Levada, P. M. [UNESP]
Zanotti-Magalhaes, E. M.
Magalhaes, L. A.
Ribeiro-Paes, J. T. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Universidade Estadual de Campinas (UNICAMP)
ADR
Univ Paulista
dc.contributor.author.fl_str_mv Oliveira, A. L. D.
Levada, P. M. [UNESP]
Zanotti-Magalhaes, E. M.
Magalhaes, L. A.
Ribeiro-Paes, J. T. [UNESP]
dc.subject.por.fl_str_mv Biomphalaria tenagophila
Schistosoma mansoni
Hemocyte behavior
Schistosomiasis
Biological control
topic Biomphalaria tenagophila
Schistosoma mansoni
Hemocyte behavior
Schistosomiasis
Biological control
description The relationships between schistosomiasis and its intermediate host, mollusks of the genus Biomphalaria, have been a concern for decades. It is known that the vector mollusk shows different susceptibility against parasite infection, whose occurrence depends on the interaction between the forms of trematode larvae and the host defense cells. These cells are called amebocytes or hemocytes and are responsible for the recognition of foreign bodies and for phagocytosis and cytotoxic reactions. The defense cells mediate the modulation of the resistant and susceptible phenotypes of the mollusk. Two main types of hemocytes are found in the Biomphalaria hemolymph: the granulocytes and the hyalinocytes. We studied the variation in the number (kinetics) of hemocytes for 24 h after exposing the parasite to genetically selected and non-selected strains of Biomphalaria tenagophila, susceptible or not to infection by Schistosoma mansoni. The differences were analyzed referred to the variations in the number of hemocytes in mollusks susceptible or not to infection by S. mansoni. The hemolymph of the selected and non-selected snails was collected, and hemocytes were counted using a Neubauer chamber at six designated periods: 0 h (control, non-exposed individuals), 2 h, 6 h, 12 h, 18 h and, 24 h after parasite exposure. Samples of hemolymph of five selected mollusks and five non-selected mollusks were separately used at each counting time. There was a significant variation in the number of hemocytes between the strains, which indicates that defense cells have different behaviors in resistant and susceptible mollusks.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
2014-05-20T13:22:20Z
2014-05-20T13:22:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4238/vol9-4gmr1143
Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 4, p. 2436-2445, 2010.
1676-5680
http://hdl.handle.net/11449/6536
10.4238/vol9-4gmr1143
WOS:000285718400020
WOS000285718400020.pdf
url http://dx.doi.org/10.4238/vol9-4gmr1143
http://hdl.handle.net/11449/6536
identifier_str_mv Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 4, p. 2436-2445, 2010.
1676-5680
10.4238/vol9-4gmr1143
WOS:000285718400020
WOS000285718400020.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Research
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dc.format.none.fl_str_mv 2436-2445
application/pdf
dc.publisher.none.fl_str_mv Funpec-editora
publisher.none.fl_str_mv Funpec-editora
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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