Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12625 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. |
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Lima Junior, Raimundo SousaMello, Cintia da SilvaSiani, Antonio CarlosValente, Ligia M. MarinoKubelka, Claire Fernandes2016-01-26T15:21:09Z2016-01-26T15:21:09Z2013LIMA JUNIOR, Raimundo Sousa; et al. Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus. Natural Product Comuniations, v.8, n.0, 4p, 2013.1555-9475https://www.arca.fiocruz.br/handle/icict/12625engNatural Product CommunicationsUncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia de Fármacos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Dengue is the major arbovirus in the world annually causing morbidity and death. The severe dengue is associated with changes on the endothelial barrier function due to the production of inflammatory mediators by immune cells and by the endothelium. Dengue virus (DENV) replicates efficiently in human endothelial cells in vitro and elicits immune responses resulting in endothelial permeability. Uncaria tomentosa (Willd.) DC.(Rubiaceae), known as cat’s claw, has been used in folk medicine for the treatment of a wide-array of symptoms and several scientific studies reported its antiviral, immunomodulatory, anti-inflammatory and antioxidant properties. Here we infected a human lineage of dermal microvascular endothelial cells (HMEC-1) with DENV-2 and treated it with an alkaloidal fraction from U. tomentosa bark (AFUT). We showed antiviral and immunomodulatory activities of U. tomentosa by determining the NS1 antigen and IL-8 in supernatant of DENV-2 infected HMEC-1. Furthermore, by measurement of transendothelial electrical resistance (TEER) we demonstrated for the first time that a plant derivative contributed to reduce paracellular permeability in DENV-2 infected HMEC-1. We also showed that IL-8 contributed significantly to the induction of permeability. Further studies are still required until a new drug can be indicated, but our in vitro results support the evidences that AFUT could be potentially useful to preventive treatment of severe dengue.Uncaria tomentosaDengueAntiviralOxindole alkaloidEndothelial cellsPermeabilityCélulas EndoteliaisUnha-de-GatoDengueAntiviraisPermeabilityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12625/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALraimundo_limajr_etal_IOC_2013.pdfraimundo_limajr_etal_IOC_2013.pdfapplication/pdf461335https://www.arca.fiocruz.br/bitstream/icict/12625/2/raimundo_limajr_etal_IOC_2013.pdf7e6ff769450a5d3683e8f9c71c1cf5deMD52TEXTraimundo_limajr_etal_IOC_2013.pdf.txtraimundo_limajr_etal_IOC_2013.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus |
| title |
Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus |
| spellingShingle |
Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus Lima Junior, Raimundo Sousa Uncaria tomentosa Dengue Antiviral Oxindole alkaloid Endothelial cells Permeability Células Endoteliais Unha-de-Gato Dengue Antivirais Permeability |
| title_short |
Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus |
| title_full |
Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus |
| title_fullStr |
Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus |
| title_full_unstemmed |
Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus |
| title_sort |
Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus |
| author |
Lima Junior, Raimundo Sousa |
| author_facet |
Lima Junior, Raimundo Sousa Mello, Cintia da Silva Siani, Antonio Carlos Valente, Ligia M. Marino Kubelka, Claire Fernandes |
| author_role |
author |
| author2 |
Mello, Cintia da Silva Siani, Antonio Carlos Valente, Ligia M. Marino Kubelka, Claire Fernandes |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Lima Junior, Raimundo Sousa Mello, Cintia da Silva Siani, Antonio Carlos Valente, Ligia M. Marino Kubelka, Claire Fernandes |
| dc.subject.en.pt_BR.fl_str_mv |
Uncaria tomentosa Dengue Antiviral Oxindole alkaloid Endothelial cells Permeability |
| topic |
Uncaria tomentosa Dengue Antiviral Oxindole alkaloid Endothelial cells Permeability Células Endoteliais Unha-de-Gato Dengue Antivirais Permeability |
| dc.subject.decs.pt_BR.fl_str_mv |
Células Endoteliais Unha-de-Gato Dengue Antivirais Permeability |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2013 |
| dc.date.issued.fl_str_mv |
2013 |
| dc.date.accessioned.fl_str_mv |
2016-01-26T15:21:09Z |
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2016-01-26T15:21:09Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
LIMA JUNIOR, Raimundo Sousa; et al. Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus. Natural Product Comuniations, v.8, n.0, 4p, 2013. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/12625 |
| dc.identifier.issn.none.fl_str_mv |
1555-9475 |
| identifier_str_mv |
LIMA JUNIOR, Raimundo Sousa; et al. Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus. Natural Product Comuniations, v.8, n.0, 4p, 2013. 1555-9475 |
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https://www.arca.fiocruz.br/handle/icict/12625 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Natural Product Communications |
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Natural Product Communications |
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