Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations

Detalhes bibliográficos
Autor(a) principal: Oliveira, Valéria da Costa
Data de Publicação: 2017
Outros Autores: Boechat, Viviane Cardoso, Mendes Junior, Artur Augusto Velho, Madeira, Maria de Fátima, Ferreira, Luiz Claudio, Figueiredo, Fabiano Borges, Campos, Monique Paiva, Rodrigues, Francisco das Chagas de Carvalho, Oliveira, Raquel de Vasconcellos Carvalhaes de, Amendoeira, Maria Regina Reis, Menezes, Rodrigo Caldas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23197
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.
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spelling Oliveira, Valéria da CostaBoechat, Viviane CardosoMendes Junior, Artur Augusto VelhoMadeira, Maria de FátimaFerreira, Luiz ClaudioFigueiredo, Fabiano BorgesCampos, Monique PaivaRodrigues, Francisco das Chagas de CarvalhoOliveira, Raquel de Vasconcellos Carvalhaes deAmendoeira, Maria Regina ReisMenezes, Rodrigo Caldas2017-11-16T11:25:00Z2017-11-16T11:25:00Z2017OLIVEIRA, Valéria da Costa; et al. Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations. Plos One, v.12, n.4, e0175588, 15p, Apr. 2017.1932-6203https://www.arca.fiocruz.br/handle/icict/2319710.1371/journal.pone.0175588engPublic Library of ScienceLeishmania infantumZoonosesLeishmaniose VisceralSistema Nervoso CentralLeishmania infantumcentral nervous systemZoonotic visceral leishmaniasisOccurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Epidemiologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.Zoonotic visceral leishmaniasis is caused by the protozoan Leishmania infantum and little is known about the occurrence and pathogenesis of this parasite in the CNS. The aims of this study were to evaluate the occurrence, viability and load of L. infantum in the CNS, and to identify the neurological histological alterations associated with this protozoan and its co-infections in naturally infected dogs. Forty-eight Leishmania-seropositive dogs from which L. infantum was isolated after necropsy were examined. Cerebrospinal fluid (CSF) samples were analyzed by parasitological culture, quantitative real-time PCR (qPCR) and the rapid immunochromatographic Dual Path Platform test. Brain, spinal cord and spleen samples were submitted to parasitological culture, qPCR, and histological techniques. Additionally, anti-Toxoplasma gondii and anti-Ehrlichia canis antibodies in serum and distemper virus antigens in CSF were investigated. None of the dogs showed neurological signs. All dogs tested positive for L. infantum in the CNS. Viable forms of L. infantum were isolated from CSF, brain and spinal cord in 25% of the dogs. Anti-L. infantum antibodies were detected in CSF in 61% of 36 dogs. Inflammatory histological alterations were observed in the CNS of 31% of the animals; of these, 66% were seropositive for E. canis and/or T. gondii. Amastigote forms were associated with granulomatous non-suppurative encephalomyelitis in a dog without evidence of co-infections. The highest frequency of L. infantum DNA was observed in the brain (98%), followed by the spinal cord (96%), spleen (95%), and CSF (50%). The highest L. infantum load in CNS was found in the spinal cord. These results demonstrate that L. infantum can cross the blood-brain barrier, spread through CSF, and cause active infection in the entire CNS of dogs. Additionally, L. infantum can cause inflammation in the CNS that can lead to neurological signs with progression of the disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23197/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmaria_amendoeira_etal_IOC_2017.pdfmaria_amendoeira_etal_IOC_2017.pdfapplication/pdf3406658https://www.arca.fiocruz.br/bitstream/icict/23197/2/maria_amendoeira_etal_IOC_2017.pdfb56818c4923ad9b09eb9ca36b7069392MD52TEXTmaria_amendoeira_etal_IOC_2017.pdf.txtmaria_amendoeira_etal_IOC_2017.pdf.txtExtracted texttext/plain53914https://www.arca.fiocruz.br/bitstream/icict/23197/3/maria_amendoeira_etal_IOC_2017.pdf.txtb83b8b3d040219bdf5585ab37d10815aMD53icict/231972018-08-15 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dc.title.pt_BR.fl_str_mv Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations
title Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations
spellingShingle Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations
Oliveira, Valéria da Costa
Leishmania infantum
Zoonoses
Leishmaniose Visceral
Sistema Nervoso Central
Leishmania infantum
central nervous system
Zoonotic visceral leishmaniasis
title_short Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations
title_full Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations
title_fullStr Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations
title_full_unstemmed Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations
title_sort Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations
author Oliveira, Valéria da Costa
author_facet Oliveira, Valéria da Costa
Boechat, Viviane Cardoso
Mendes Junior, Artur Augusto Velho
Madeira, Maria de Fátima
Ferreira, Luiz Claudio
Figueiredo, Fabiano Borges
Campos, Monique Paiva
Rodrigues, Francisco das Chagas de Carvalho
Oliveira, Raquel de Vasconcellos Carvalhaes de
Amendoeira, Maria Regina Reis
Menezes, Rodrigo Caldas
author_role author
author2 Boechat, Viviane Cardoso
Mendes Junior, Artur Augusto Velho
Madeira, Maria de Fátima
Ferreira, Luiz Claudio
Figueiredo, Fabiano Borges
Campos, Monique Paiva
Rodrigues, Francisco das Chagas de Carvalho
Oliveira, Raquel de Vasconcellos Carvalhaes de
Amendoeira, Maria Regina Reis
Menezes, Rodrigo Caldas
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Valéria da Costa
Boechat, Viviane Cardoso
Mendes Junior, Artur Augusto Velho
Madeira, Maria de Fátima
Ferreira, Luiz Claudio
Figueiredo, Fabiano Borges
Campos, Monique Paiva
Rodrigues, Francisco das Chagas de Carvalho
Oliveira, Raquel de Vasconcellos Carvalhaes de
Amendoeira, Maria Regina Reis
Menezes, Rodrigo Caldas
dc.subject.other.pt_BR.fl_str_mv Leishmania infantum
Zoonoses
Leishmaniose Visceral
Sistema Nervoso Central
topic Leishmania infantum
Zoonoses
Leishmaniose Visceral
Sistema Nervoso Central
Leishmania infantum
central nervous system
Zoonotic visceral leishmaniasis
dc.subject.en.pt_BR.fl_str_mv Leishmania infantum
central nervous system
Zoonotic visceral leishmaniasis
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-11-16T11:25:00Z
dc.date.available.fl_str_mv 2017-11-16T11:25:00Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Valéria da Costa; et al. Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations. Plos One, v.12, n.4, e0175588, 15p, Apr. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23197
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0175588
identifier_str_mv OLIVEIRA, Valéria da Costa; et al. Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations. Plos One, v.12, n.4, e0175588, 15p, Apr. 2017.
1932-6203
10.1371/journal.pone.0175588
url https://www.arca.fiocruz.br/handle/icict/23197
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
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