Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni

Detalhes bibliográficos
Autor(a) principal: Faro, Marta Julia
Data de Publicação: 2013
Outros Autores: Perazzini, Mariana, Corrêa, Lygia dos Reis, Silva, Clélia Christina Mello, Pinheiro, Jairo, Mota, Ester Maria, Souza, Samaly de, Andrade, Zilton de Araújo, Maldonado Júnior, Arnaldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14880
Resumo: Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Ciências Biológicas. Rio de Janeiro, RJ, Brasil.
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spelling Faro, Marta JuliaPerazzini, MarianaCorrêa, Lygia dos ReisSilva, Clélia Christina MelloPinheiro, JairoMota, Ester MariaSouza, Samaly deAndrade, Zilton de AraújoMaldonado Júnior, Arnaldo2016-07-14T13:42:48Z2016-07-14T13:42:48Z2013FARO, Marta Julia et al. Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni. Experimental Parasitology, v. 134, p. 228–234, 2013.0014-4894https://www.arca.fiocruz.br/handle/icict/1488010.1016/j.exppara.2013.03.020engElsevierBiological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Ciências Biológicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional de Referência em Malacologia Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Departamento de Ciências Fisiológicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia, Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Salvador, BA, Brasil.Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.Parasitic castration in the snail-trematode relationship can be understood as any change in the reproductive function of the snail that is due to interference by the developing larvae inside the snail that leads to the reduction or complete disruption of egg-laying activity. This study was designed to observe the parasitic castration of Biomphalaria glabrata infected with Schistosoma mansoni during both the pre-patent and patent periods. The effect of infection on snail fecundity and fertility, growth rate and survival was studied during the 62 days following miracidia exposure. An integrated approach was employed that used biochemical and histological tools over the same period. To study the effect of infection on reproduction, we individually exposed 30 snails to 5 miracidia each and tracked their fertility and fecundity. For our histopathological studies, 50 snails were exposed to 20 miracidia each, and for our histochemical studies, 50 snails were exposed to 5 miracidia each. An equal number of uninfected snails were used as a control for each group. The B. glabrata exposed to the BH strain of S. mansoni showed 50% positivity for cercarial shedding. Both the experimental and control groups showed 100% survival. The pre-patent period lasted until 39 days after exposure to miracidia. Exposed snails that showed cercarial shedding exhibited higher growth rates than either exposed snails that did not demonstrate cercarial shedding or uninfected controls. Exposed snails without cercarial shedding and uninfected controls showed no differences in the reproductive parameters evaluated during the patent period; snails experiencing cercarial shedding showed a reduction in fecundity and fertility. These snails began to lay eggs only after the 50th day post miracidia exposure. The haemolymph glucose levels showed an oscillating pattern that decreased during periods of greater mobilisation of energy by the larvae and was accompanied by a depletion of glycogen in the cephalopodal mass and digestive gland. Histopathological examination at 55 days showed that the ovotestis was highly atrophied. There was almost complete disappearance of germ cells, and the supporting stroma formed a nearly empty net. At day 45, the infected digestive gland showed a high cylindrical epithelium with little preserved cytoplasm. The contents of the secretory granules of the albumen gland of infected animals stained with Alcian blue (AB), pH 1.0, indicating the presence of sulphated carbohydrates. Thus, parasitic castration in the B. glabrata–S. mansoni model may be regulated directly and indirectly by the developmental stage of the trematode and the biochemical and histopathological alterations during the patent period of infection.Schistosoma mansoniBiomphalaria glabrataParasitic castrationReproductionHistopathologyBiochemicalinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14880/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFaro MJ Biological....pdfFaro MJ Biological....pdfapplication/pdf3360592https://www.arca.fiocruz.br/bitstream/icict/14880/2/Faro%20MJ%20Biological....pdfe994ca3353f29b73a2c600da0a19f266MD52TEXTFaro MJ Biological....pdf.txtFaro MJ Biological....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni
title Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni
spellingShingle Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni
Faro, Marta Julia
Schistosoma mansoni
Biomphalaria glabrata
Parasitic castration
Reproduction
Histopathology
Biochemical
title_short Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni
title_full Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni
title_fullStr Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni
title_full_unstemmed Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni
title_sort Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni
author Faro, Marta Julia
author_facet Faro, Marta Julia
Perazzini, Mariana
Corrêa, Lygia dos Reis
Silva, Clélia Christina Mello
Pinheiro, Jairo
Mota, Ester Maria
Souza, Samaly de
Andrade, Zilton de Araújo
Maldonado Júnior, Arnaldo
author_role author
author2 Perazzini, Mariana
Corrêa, Lygia dos Reis
Silva, Clélia Christina Mello
Pinheiro, Jairo
Mota, Ester Maria
Souza, Samaly de
Andrade, Zilton de Araújo
Maldonado Júnior, Arnaldo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Faro, Marta Julia
Perazzini, Mariana
Corrêa, Lygia dos Reis
Silva, Clélia Christina Mello
Pinheiro, Jairo
Mota, Ester Maria
Souza, Samaly de
Andrade, Zilton de Araújo
Maldonado Júnior, Arnaldo
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Biomphalaria glabrata
Parasitic castration
Reproduction
Histopathology
Biochemical
topic Schistosoma mansoni
Biomphalaria glabrata
Parasitic castration
Reproduction
Histopathology
Biochemical
description Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Ciências Biológicas. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-07-14T13:42:48Z
dc.date.available.fl_str_mv 2016-07-14T13:42:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARO, Marta Julia et al. Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni. Experimental Parasitology, v. 134, p. 228–234, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14880
dc.identifier.issn.pt_BR.fl_str_mv 0014-4894
dc.identifier.doi.none.fl_str_mv 10.1016/j.exppara.2013.03.020
identifier_str_mv FARO, Marta Julia et al. Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni. Experimental Parasitology, v. 134, p. 228–234, 2013.
0014-4894
10.1016/j.exppara.2013.03.020
url https://www.arca.fiocruz.br/handle/icict/14880
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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