Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping

Detalhes bibliográficos
Autor(a) principal: Nicolau, Carolina Alves
Data de Publicação: 2018
Outros Autores: Prorock, Alyson, Bao, Yongde, Ferreira, Ana Gisele da Costa Neves, Valente, Richard Hemmi, Fox, Jay William
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/29403
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil / University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA.
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spelling Nicolau, Carolina AlvesProrock, AlysonBao, YongdeFerreira, Ana Gisele da Costa NevesValente, Richard HemmiFox, Jay William2018-10-05T18:34:49Z2018-10-05T18:34:49Z2018NICOLAU, Carolina Alves; et al. Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping. Toxins, v.10, n.69, 26p, 2018.2072-6651https://www.arca.fiocruz.br/handle/icict/2940310.3390/toxins100200692072-6651engMDPIBothrops jararacapotencial terapêuticomapa de conectividadedescoberta de drogasmedicamentos biossimilaresBothrops jararacatherapeutic potentialconnectivity mapdrug discoverybiosimilar drugsRevisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mappinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil / University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA.University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA.University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil.University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA.Snake venoms are sources of molecules with proven and potential therapeutic applications. However, most activities assayed in venoms (or their components) are of hemorrhagic, hypotensive, edematogenic, neurotoxic or myotoxic natures. Thus, other relevant activities might remain unknown. Using functional genomics coupled to the connectivity map (C-map) approach, we undertook a wide range indirect search for biological activities within the venom of the South American pit viper Bothrops jararaca. For that effect, venom was incubated with human breast adenocarcinoma cell line (MCF7) followed by RNA extraction and gene expression analysis. A list of 90 differentially expressed genes was submitted to biosimilar drug discovery based on pattern recognition. Among the 100 highest-ranked positively correlated drugs, only the antihypertensive, antimicrobial (both antibiotic and antiparasitic), and antitumor classes had been previously reported for B. jararaca venom. The majority of drug classes identified were related to (1) antimicrobial activity; (2) treatment of neuropsychiatric illnesses (Parkinson's disease, schizophrenia, depression, and epilepsy); (3) treatment of cardiovascular diseases, and (4) anti-inflammatory action. The C-map results also indicated that B. jararaca venom may have components that target G-protein-coupled receptors (muscarinic, serotonergic, histaminergic, dopaminergic, GABA, and adrenergic) and ion channels. Although validation experiments are still necessary, the C-map correlation to drugs with activities previously linked to snake venoms supports the efficacy of this strategy as a broad-spectrum approach for biological activity screening, and rekindles the snake venom-based search for new therapeutic agents.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29403/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALcarolinaAlves_nicolau_etal_IOC_2081.pdfcarolinaAlves_nicolau_etal_IOC_2081.pdfapplication/pdf945357https://www.arca.fiocruz.br/bitstream/icict/29403/2/carolinaAlves_nicolau_etal_IOC_2081.pdfbef37e6da5b6ecb11075862afbd0bbeeMD52TEXTcarolinaAlves_nicolau_etal_IOC_2081.pdf.txtcarolinaAlves_nicolau_etal_IOC_2081.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping
title Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping
spellingShingle Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping
Nicolau, Carolina Alves
Bothrops jararaca
potencial terapêutico
mapa de conectividade
descoberta de drogas
medicamentos biossimilares
Bothrops jararaca
therapeutic potential
connectivity map
drug discovery
biosimilar drugs
title_short Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping
title_full Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping
title_fullStr Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping
title_full_unstemmed Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping
title_sort Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping
author Nicolau, Carolina Alves
author_facet Nicolau, Carolina Alves
Prorock, Alyson
Bao, Yongde
Ferreira, Ana Gisele da Costa Neves
Valente, Richard Hemmi
Fox, Jay William
author_role author
author2 Prorock, Alyson
Bao, Yongde
Ferreira, Ana Gisele da Costa Neves
Valente, Richard Hemmi
Fox, Jay William
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Nicolau, Carolina Alves
Prorock, Alyson
Bao, Yongde
Ferreira, Ana Gisele da Costa Neves
Valente, Richard Hemmi
Fox, Jay William
dc.subject.other.pt_BR.fl_str_mv Bothrops jararaca
potencial terapêutico
mapa de conectividade
descoberta de drogas
medicamentos biossimilares
topic Bothrops jararaca
potencial terapêutico
mapa de conectividade
descoberta de drogas
medicamentos biossimilares
Bothrops jararaca
therapeutic potential
connectivity map
drug discovery
biosimilar drugs
dc.subject.en.pt_BR.fl_str_mv Bothrops jararaca
therapeutic potential
connectivity map
drug discovery
biosimilar drugs
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil / University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-10-05T18:34:49Z
dc.date.available.fl_str_mv 2018-10-05T18:34:49Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NICOLAU, Carolina Alves; et al. Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping. Toxins, v.10, n.69, 26p, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/29403
dc.identifier.issn.pt_BR.fl_str_mv 2072-6651
dc.identifier.doi.none.fl_str_mv 10.3390/toxins10020069
dc.identifier.eissn.none.fl_str_mv 2072-6651
identifier_str_mv NICOLAU, Carolina Alves; et al. Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping. Toxins, v.10, n.69, 26p, 2018.
2072-6651
10.3390/toxins10020069
url https://www.arca.fiocruz.br/handle/icict/29403
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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