Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection

Detalhes bibliográficos
Autor(a) principal: Barbosa, Rafael Polidoro Alves
Data de Publicação: 2013
Outros Autores: Galvão Filho, Bruno, Santos, Luara Isabela dos, Sales Junior, Policarpo Ademar, Marques, Pedro Elias, Pereira, Rafaela Vaz Sousa, Cara, Denise Carmona, Bruna-Romero, Oscar, Rodrigues, Maurício Martins, Gazzinelli, Ricardo Tostes, Machado, Alexandre Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6509
Resumo: FIOCRUZ/PDTIS, CNPq/MAPA/DAS, FAPEMIG, CNPq
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spelling Barbosa, Rafael Polidoro AlvesGalvão Filho, BrunoSantos, Luara Isabela dosSales Junior, Policarpo AdemarMarques, Pedro EliasPereira, Rafaela Vaz SousaCara, Denise CarmonaBruna-Romero, OscarRodrigues, Maurício MartinsGazzinelli, Ricardo TostesMachado, Alexandre Vieira2013-05-21T13:56:48Z2013-05-21T13:56:48Z2013BARBOSA, Rafael Polidoro Alves et al. Vaccination using recombinants rnfluenza and Adenoviruses encoding amastigote surface protein-2 Are highly effective on protection against Trypanosoma cruzi infection. PLoS ONE, v. 8, n. 4, p. 1-11, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/6509FIOCRUZ/PDTIS, CNPq/MAPA/DAS, FAPEMIG, CNPqUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular (CTCMol). São Paulo, SP, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou Belo Horizonte, MG, Brasil/ University of Massachusetts Medical School. Division of Infectious Diseases and Immunology. Department of Medicine. Worcester, MA, USAFundação Oswaldo Cruz. Centro de Pesquisas René Rachou Belo Horizonte, MG, Brasil.In the present study we evaluated the protection raised by immunization with recombinant influenza viruses carrying sequences coding for polypeptides corresponding to medial and carboxi-terminal moieties of Trypanosoma cruzis amastigote surface protein 2 (ASP2). Those viruses were used in sequential immunization with recombinant adenovirus (heterologous prime-boost immunization protocol) encoding the complete sequence of ASP2 (Ad-ASP2) in two mouse strains (C57BL/6 and C3H/He). The CD8 effector response elicited by this protocol was comparable to that observed in mice immunized twice with Ad-ASP2 and more robust than that observed in mice that were immunized once with Ad-ASP2. Where as a single immunization with Ad-ASP2 sufficed to completely protect C57BL/6 mice, a higher survival rate was observed in C3H/He mice that were primed with recombinant influenza virus and boosted with Ad-ASP2 after being challenged withT. cruzi. Analyzing the phenotype of CD8+T cells obtained from spleen of vaccinated C3H/He mice we observed that heterologous prime-boost immunization protocol elicited more CD8+ T cells specific for the immunodominant epitope as well as a higher number of CD8+ T cells producing TNF-a and IFN-c and a higher mobilization of surface marker CD107a. Taken together, our results suggest that immunodominant subpopulations of CD8+T elicited after immunization could be directly related to degree of protection achieved by different immunization protocols using different viral vectors. Overall, these results demonstrated the usefulness of recombinant influenza viruses in immunization protocols against Chagas Disease.engVaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTrypanosoma cruzi/infectionChagas Diseaseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALVaccination Using Recombinants Influenza and Adenoviruses Encoding.pdfVaccination Using Recombinants Influenza and Adenoviruses Encoding.pdfapplication/pdf2069588https://www.arca.fiocruz.br/bitstream/icict/6509/1/Vaccination%20Using%20Recombinants%20Influenza%20and%20Adenoviruses%20Encoding.pdf8d22d327fce97ba5c5da1ca25ce64974MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/6509/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTVaccination Using Recombinants Influenza and Adenoviruses Encoding.pdf.txtVaccination Using Recombinants Influenza and Adenoviruses Encoding.pdf.txtExtracted texttext/plain56745https://www.arca.fiocruz.br/bitstream/icict/6509/5/Vaccination%20Using%20Recombinants%20Influenza%20and%20Adenoviruses%20Encoding.pdf.txt1378d062408bffe9b22aa831d44125d9MD55THUMBNAILVaccination Using Recombinants Influenza and Adenoviruses Encoding.pdf.jpgVaccination Using Recombinants Influenza and Adenoviruses Encoding.pdf.jpgGenerated Thumbnailimage/jpeg2253https://www.arca.fiocruz.br/bitstream/icict/6509/4/Vaccination%20Using%20Recombinants%20Influenza%20and%20Adenoviruses%20Encoding.pdf.jpg457bda54d358854e334da9413ec89679MD54icict/65092018-05-15 16:50:27.352oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-05-15T19:50:27Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection
title Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection
spellingShingle Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection
Barbosa, Rafael Polidoro Alves
Trypanosoma cruzi/infection
Chagas Disease
title_short Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection
title_full Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection
title_fullStr Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection
title_full_unstemmed Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection
title_sort Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection
author Barbosa, Rafael Polidoro Alves
author_facet Barbosa, Rafael Polidoro Alves
Galvão Filho, Bruno
Santos, Luara Isabela dos
Sales Junior, Policarpo Ademar
Marques, Pedro Elias
Pereira, Rafaela Vaz Sousa
Cara, Denise Carmona
Bruna-Romero, Oscar
Rodrigues, Maurício Martins
Gazzinelli, Ricardo Tostes
Machado, Alexandre Vieira
author_role author
author2 Galvão Filho, Bruno
Santos, Luara Isabela dos
Sales Junior, Policarpo Ademar
Marques, Pedro Elias
Pereira, Rafaela Vaz Sousa
Cara, Denise Carmona
Bruna-Romero, Oscar
Rodrigues, Maurício Martins
Gazzinelli, Ricardo Tostes
Machado, Alexandre Vieira
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Rafael Polidoro Alves
Galvão Filho, Bruno
Santos, Luara Isabela dos
Sales Junior, Policarpo Ademar
Marques, Pedro Elias
Pereira, Rafaela Vaz Sousa
Cara, Denise Carmona
Bruna-Romero, Oscar
Rodrigues, Maurício Martins
Gazzinelli, Ricardo Tostes
Machado, Alexandre Vieira
dc.subject.decs.pt_BR.fl_str_mv Trypanosoma cruzi/infection
Chagas Disease
topic Trypanosoma cruzi/infection
Chagas Disease
description FIOCRUZ/PDTIS, CNPq/MAPA/DAS, FAPEMIG, CNPq
publishDate 2013
dc.date.accessioned.fl_str_mv 2013-05-21T13:56:48Z
dc.date.available.fl_str_mv 2013-05-21T13:56:48Z
dc.date.issued.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, Rafael Polidoro Alves et al. Vaccination using recombinants rnfluenza and Adenoviruses encoding amastigote surface protein-2 Are highly effective on protection against Trypanosoma cruzi infection. PLoS ONE, v. 8, n. 4, p. 1-11, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6509
dc.identifier.issn.none.fl_str_mv 1932-6203
identifier_str_mv BARBOSA, Rafael Polidoro Alves et al. Vaccination using recombinants rnfluenza and Adenoviruses encoding amastigote surface protein-2 Are highly effective on protection against Trypanosoma cruzi infection. PLoS ONE, v. 8, n. 4, p. 1-11, 2013.
1932-6203
url https://www.arca.fiocruz.br/handle/icict/6509
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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