Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Forms
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15559 |
Resumo: | Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil. |
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Kian, DanielleLancheros, César Armando ContrerasCampos, Igor AlexandreFernandes, Tamiris Zanforlin OlmosPinge-Filho, PhilenoSantos, Márcia Regina Machado dosSilveira Filho, José Franco daNakamura, Celso VataruSilva, João Santana daOgatta, Sueli Fumie YamadaLioni, Lucy M. Yamauchi2016-08-30T17:21:12Z2016-08-30T17:21:12Z2015KIAN, Danielle et al. Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms. Korean Journal of Parasitology, v. 53, n. 4, p. 483–488, 2015.0023-4001https://www.arca.fiocruz.br/handle/icict/1555910.3347/kjp.2015.53.4.483porMolecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Formsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunobiologia e Parasitologia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Bandeirante de São Paulo. Conselho de Pós-graduação e Pesquisa. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunobiologia e Parasitologia. Londrina, PR, Brasil.Universidade Estadual de Maringá. Centro de Ciências da Saúde. Departamento de Ciências Básicas da Saúde. Maringá, PR, Brasil.Universidade de São Paulo. Departamento de Parasitologia, Microbiologia e Imunologia. Ribeirão Preto, SP, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil.This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.Tc8.2 expressionTrypanosoma cruziDual localizationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15559/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMolecular characterization of Trypanosoma cruzi Tc8.2 gene.pdfMolecular characterization of Trypanosoma cruzi Tc8.2 gene.pdfapplication/pdf630488https://www.arca.fiocruz.br/bitstream/icict/15559/2/Molecular%20characterization%20of%20Trypanosoma%20cruzi%20Tc8.2%20gene.pdf8888e97da01447ee45235bf2ed02d7b2MD52TEXTMolecular characterization of Trypanosoma cruzi Tc8.2 gene.pdf.txtMolecular characterization of Trypanosoma cruzi Tc8.2 gene.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Forms |
| title |
Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Forms |
| spellingShingle |
Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Forms Kian, Danielle Tc8.2 expression Trypanosoma cruzi Dual localization |
| title_short |
Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Forms |
| title_full |
Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Forms |
| title_fullStr |
Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Forms |
| title_full_unstemmed |
Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Forms |
| title_sort |
Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and trypomastigote Forms |
| author |
Kian, Danielle |
| author_facet |
Kian, Danielle Lancheros, César Armando Contreras Campos, Igor Alexandre Fernandes, Tamiris Zanforlin Olmos Pinge-Filho, Phileno Santos, Márcia Regina Machado dos Silveira Filho, José Franco da Nakamura, Celso Vataru Silva, João Santana da Ogatta, Sueli Fumie Yamada Lioni, Lucy M. Yamauchi |
| author_role |
author |
| author2 |
Lancheros, César Armando Contreras Campos, Igor Alexandre Fernandes, Tamiris Zanforlin Olmos Pinge-Filho, Phileno Santos, Márcia Regina Machado dos Silveira Filho, José Franco da Nakamura, Celso Vataru Silva, João Santana da Ogatta, Sueli Fumie Yamada Lioni, Lucy M. Yamauchi |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Kian, Danielle Lancheros, César Armando Contreras Campos, Igor Alexandre Fernandes, Tamiris Zanforlin Olmos Pinge-Filho, Phileno Santos, Márcia Regina Machado dos Silveira Filho, José Franco da Nakamura, Celso Vataru Silva, João Santana da Ogatta, Sueli Fumie Yamada Lioni, Lucy M. Yamauchi |
| dc.subject.en.pt_BR.fl_str_mv |
Tc8.2 expression Trypanosoma cruzi Dual localization |
| topic |
Tc8.2 expression Trypanosoma cruzi Dual localization |
| description |
Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil. |
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2015 |
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2015 |
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2016-08-30T17:21:12Z |
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2016-08-30T17:21:12Z |
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KIAN, Danielle et al. Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms. Korean Journal of Parasitology, v. 53, n. 4, p. 483–488, 2015. |
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https://www.arca.fiocruz.br/handle/icict/15559 |
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0023-4001 |
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10.3347/kjp.2015.53.4.483 |
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KIAN, Danielle et al. Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms. Korean Journal of Parasitology, v. 53, n. 4, p. 483–488, 2015. 0023-4001 10.3347/kjp.2015.53.4.483 |
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