A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo de conferência |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23784 |
| Resumo: | Chembio Diagnostic Systems. Medford, United States of America |
| id |
CRUZ_fb6f69fb3bb12ee1e99155a4c8b2ec6e |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/23784 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Lyashchenko, Konstantin PGreenwald, RenaEsfandiari, JavanNabity, Scott ARibeiro, Guilherme de SousaMedeiros, Marco AlbertoReis, Mitermayer Galvão dosKo, Albert Icksang2017-12-27T13:11:10Z2017-12-27T13:11:10Z2012LYASHCHENKO, K. P. et al. A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses. In: CONGRESSO INTERNACIONAL DE MEDICINA TROPICAL E MALÁRIA, 18 e CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 48., 2012, Rio de Janeiro. Resumos... Rio de Janeiro: Sociedade Brasileira de Medicina Tropical, 2012.https://www.arca.fiocruz.br/handle/icict/23784engSociedade Brasileira de Medicina TropicalLeptospiroseSaúde públicaHumanosRealização de testesDiagnósticoLeptospirosisPublic healthHumansConducting testsDiagnosticA novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responsesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectChembio Diagnostic Systems. Medford, United States of AmericaChembio Diagnostic Systems. Medford, United States of AmericaDuke University School of Medicine. Durham, United States of AmericaDuke University School of Medicine. Durham, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, BrasilFundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale University Schools of Public Health and Medicine. New Haven, United States of AmericaIntroduction: Improved diagnostics for leptospirosis are urgently needed, especially for resource-poor countries where leptospirosis is a major public health problem. The gold standard serodiagnostic test, the microagglutination test or MAT, is complex, time-consuming, labor-intensive, and unavailable in remote settings. Materials and Methods: A rapid (20 minutes) serodiagnostic test for leptospirosis was developed using recombinant leptospiral immunoglobulin-like (Lig) protein fragments and a modification of the Dual Path Platform (DPP) technology, namely dual DPP (D-DPP). The distinct feature of this new immunoassay format was that the test detects separately IgM and IgG antibodies such that each response can be measured semi-quantitatively by a reflectance reader device. The test was evaluated in a pilot study using acute (n=91) and convalescent-phase (n=96) serum samples from patients with confirmed leptospirosis and control individuals (n=50) from Brazil. Results: In this evaluation study, the D-DPP assay detected 80% of patients with acute-phase leptospirosis and 100% of patients with convalescent leptospirosis. The specificity was 100%. Among acute-phase cases, 60% developed both detectable IgM and IgG antibodies, 13% had only IgM, while 7% produced only IgG antibodies. During convalescence, 77%, 0%, and 23% of leptospirosis patients developed IgM and IgG, only IgM, and only IgG antibodies, respectively. Conclusions: We designed a recombinant Lig proteinbased D-DPP assay which can rapidly identify patients with acute-phase or convalescent leptospirosis with high accuracy. The ability to detect IgM and IgG antibodies separately improves diagnostic specificity as well as detection of patients who present at different times in the course of illness. The test therefore has the potential to provide effective point-of-care diagnosis in field settings in Brazil and resource-poor countries worldwide where leptospirosis is an emerging health probleminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23784/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLyashchenko KP A novel...SBMT 2012 Lept8.pdfLyashchenko KP A novel...SBMT 2012 Lept8.pdfapplication/pdf70207https://www.arca.fiocruz.br/bitstream/icict/23784/2/Lyashchenko%20KP%20A%20novel...SBMT%202012%20Lept8.pdfc7212ac4a88468e4dffc4cdf2bc5c6d5MD52TEXTLyashchenko KP A novel...SBMT 2012 Lept8.pdf.txtLyashchenko KP A novel...SBMT 2012 Lept8.pdf.txtExtracted texttext/plain2699https://www.arca.fiocruz.br/bitstream/icict/23784/3/Lyashchenko%20KP%20A%20novel...SBMT%202012%20Lept8.pdf.txtffaa10e6078e7d13a3418c1276e3b574MD53icict/237842019-06-26 10:52:20.344oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-26T13:52:20Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses |
| title |
A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses |
| spellingShingle |
A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses Lyashchenko, Konstantin P Leptospirose Saúde pública Humanos Realização de testes Diagnóstico Leptospirosis Public health Humans Conducting tests Diagnostic |
| title_short |
A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses |
| title_full |
A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses |
| title_fullStr |
A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses |
| title_full_unstemmed |
A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses |
| title_sort |
A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses |
| author |
Lyashchenko, Konstantin P |
| author_facet |
Lyashchenko, Konstantin P Greenwald, Rena Esfandiari, Javan Nabity, Scott A Ribeiro, Guilherme de Sousa Medeiros, Marco Alberto Reis, Mitermayer Galvão dos Ko, Albert Icksang |
| author_role |
author |
| author2 |
Greenwald, Rena Esfandiari, Javan Nabity, Scott A Ribeiro, Guilherme de Sousa Medeiros, Marco Alberto Reis, Mitermayer Galvão dos Ko, Albert Icksang |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lyashchenko, Konstantin P Greenwald, Rena Esfandiari, Javan Nabity, Scott A Ribeiro, Guilherme de Sousa Medeiros, Marco Alberto Reis, Mitermayer Galvão dos Ko, Albert Icksang |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospirose Saúde pública Humanos Realização de testes Diagnóstico |
| topic |
Leptospirose Saúde pública Humanos Realização de testes Diagnóstico Leptospirosis Public health Humans Conducting tests Diagnostic |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospirosis Public health Humans Conducting tests Diagnostic |
| description |
Chembio Diagnostic Systems. Medford, United States of America |
| publishDate |
2012 |
| dc.date.issued.fl_str_mv |
2012 |
| dc.date.accessioned.fl_str_mv |
2017-12-27T13:11:10Z |
| dc.date.available.fl_str_mv |
2017-12-27T13:11:10Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
| format |
conferenceObject |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
LYASHCHENKO, K. P. et al. A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses. In: CONGRESSO INTERNACIONAL DE MEDICINA TROPICAL E MALÁRIA, 18 e CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 48., 2012, Rio de Janeiro. Resumos... Rio de Janeiro: Sociedade Brasileira de Medicina Tropical, 2012. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/23784 |
| identifier_str_mv |
LYASHCHENKO, K. P. et al. A novel point-of-care test for leptospirosis based on Dual Path Platform (DPP) technology to differentiate IgM and IgG antibody responses. In: CONGRESSO INTERNACIONAL DE MEDICINA TROPICAL E MALÁRIA, 18 e CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 48., 2012, Rio de Janeiro. Resumos... Rio de Janeiro: Sociedade Brasileira de Medicina Tropical, 2012. |
| url |
https://www.arca.fiocruz.br/handle/icict/23784 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical |
| publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/23784/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/23784/2/Lyashchenko%20KP%20A%20novel...SBMT%202012%20Lept8.pdf https://www.arca.fiocruz.br/bitstream/icict/23784/3/Lyashchenko%20KP%20A%20novel...SBMT%202012%20Lept8.pdf.txt |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 c7212ac4a88468e4dffc4cdf2bc5c6d5 ffaa10e6078e7d13a3418c1276e3b574 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1827777104620552192 |
