Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype

Detalhes bibliográficos
Autor(a) principal: Sousa, Lourimar Viana Nascimento Franco de
Data de Publicação: 2016
Outros Autores: Santos, Vera Lúcia dos, Monteiro, Andrea de Souza, Souza, Marcus Vinicíus Dias, Marques, Sirlei Garcia, Faria, Elaine Speziali de, Assunção, Elaine Alves de Oliveira, Santos, Simone Gonçalves dos, Zonis, Juan Moises, Alvarenga, Daniel Gomes de, Holanda, Rodrigo Assunção de, Sousa, Jaqueline Gontijo de, Santos, Kênia Valéria dos, Stoianoff, Maria Aparecida de Resende
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15309
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Laboratorio de Micologia. Belo Horizonte, MG, Brasil/Universidade Vale do Rio Doce. Laboratorio de Microbiologia. Governador Valadares, MG, Brasil.
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spelling Sousa, Lourimar Viana Nascimento Franco deSantos, Vera Lúcia dosMonteiro, Andrea de SouzaSouza, Marcus Vinicíus DiasMarques, Sirlei GarciaFaria, Elaine Speziali deAssunção, Elaine Alves de OliveiraSantos, Simone Gonçalves dosZonis, Juan MoisesAlvarenga, Daniel Gomes deHolanda, Rodrigo Assunção deSousa, Jaqueline Gontijo deSantos, Kênia Valéria dosStoianoff, Maria Aparecida de Resende2016-08-18T16:05:17Z2016-08-18T16:05:17Z2016SOUSA, Lourimar Viana Nascimento Franco de et al. Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype. BMC Infect Dis., v. 16, art. 86, 2016.1471-2334https://www.arca.fiocruz.br/handle/icict/1530910.1186/s12879-016-1431-4engBiomed CentralIsolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotypeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Laboratorio de Micologia. Belo Horizonte, MG, Brasil/Universidade Vale do Rio Doce. Laboratorio de Microbiologia. Governador Valadares, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Laboratorio de Microbiologia. Belo Horizonte, MG, Brasil.Universidade CEUMA. Laboratorio de Microbiologia. São Luis, MA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Laboratorio de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal do Maranhão. Hospital Universitario. São Luis, MA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil.Universidade Vale do Rio Doce. Laboratorio de Microbiologia. Governador Valadares, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Laboratorio de Microbiologia Oral e Anaerobica. Belo Horizonte, MG, Brasil.Core Experts Oncologia. Governador Valadares, MG, BrasilLaboratorio Alvarenga. Governador Valadares, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Laboratorio de Micologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Laboratorio de Virologia Basica e Aplicada. Belo Horizonte, MG, Brasil.Federal Universidade Federal do Espirito Santo. Departamento de Patologia. Laboratorio de Bacteriologia Geral e Clinica. Centro de Ciencias da Saude. Vitória, ES, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Laboratorio de Micologia. Belo Horizonte, MG, Brasil.Background: Because of the inherent immunosuppression of cancer patients opportunistic infections by Candida spp, occur frequently. This study aimed to identify Candida species in the oral mucosa of 59 patients with orogastric cancer (OGC) and to analyze the immunological phenotype of these patients. Methods: The yeasts were identified by MALDI-TOF mass spectrometry (MS). For all isolates, we performed phospholipases and proteinases assays, in vitro adherence to buccal epithelial cells (BEC), minimum inhibitory concentration of antifungal drugs and determined the cytokine profile by Cytometric Bead Array flow citometry assay. Results: C. albicans was the most prevalent species in OGC patients (51.6 %) and control group (66.7 %). Candida spp. strains isolated from OGC patients exhibited better adherence to BEC (p = 0.05) than did the control group. Phospholipases production by Candida strains from OGC patients was lower (51.6 %) than in the control group (61.9 %). Proteinases were detected in 41.9 % and 4.8 % of the yeasts from OGC patients and control group, respectively. Significant differences were found in the serum of OGC patients compared to the control group for IL-2, IL-10, TNF-α, IFN-γ and IL-17. Conclusions: The results of this work suggest increased virulence of yeasts isolated from OGC patients and, that this may interfere with the immune phenotype.Candida sppOrogastric cancerVirulenceAntifungalImmune phenotypeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15309/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Sousa_Lourimar_Isolation_CPqRR_2016.pdfve_Sousa_Lourimar_Isolation_CPqRR_2016.pdfapplication/pdf593875https://www.arca.fiocruz.br/bitstream/icict/15309/2/ve_Sousa_Lourimar_Isolation_CPqRR_2016.pdf7f4a0472bd52fe2f7856de91d27401baMD52TEXTve_Sousa_Lourimar_Isolation_CPqRR_2016.pdf.txtve_Sousa_Lourimar_Isolation_CPqRR_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype
title Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype
spellingShingle Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype
Sousa, Lourimar Viana Nascimento Franco de
Candida spp
Orogastric cancer
Virulence
Antifungal
Immune phenotype
title_short Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype
title_full Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype
title_fullStr Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype
title_full_unstemmed Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype
title_sort Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype
author Sousa, Lourimar Viana Nascimento Franco de
author_facet Sousa, Lourimar Viana Nascimento Franco de
Santos, Vera Lúcia dos
Monteiro, Andrea de Souza
Souza, Marcus Vinicíus Dias
Marques, Sirlei Garcia
Faria, Elaine Speziali de
Assunção, Elaine Alves de Oliveira
Santos, Simone Gonçalves dos
Zonis, Juan Moises
Alvarenga, Daniel Gomes de
Holanda, Rodrigo Assunção de
Sousa, Jaqueline Gontijo de
Santos, Kênia Valéria dos
Stoianoff, Maria Aparecida de Resende
author_role author
author2 Santos, Vera Lúcia dos
Monteiro, Andrea de Souza
Souza, Marcus Vinicíus Dias
Marques, Sirlei Garcia
Faria, Elaine Speziali de
Assunção, Elaine Alves de Oliveira
Santos, Simone Gonçalves dos
Zonis, Juan Moises
Alvarenga, Daniel Gomes de
Holanda, Rodrigo Assunção de
Sousa, Jaqueline Gontijo de
Santos, Kênia Valéria dos
Stoianoff, Maria Aparecida de Resende
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Lourimar Viana Nascimento Franco de
Santos, Vera Lúcia dos
Monteiro, Andrea de Souza
Souza, Marcus Vinicíus Dias
Marques, Sirlei Garcia
Faria, Elaine Speziali de
Assunção, Elaine Alves de Oliveira
Santos, Simone Gonçalves dos
Zonis, Juan Moises
Alvarenga, Daniel Gomes de
Holanda, Rodrigo Assunção de
Sousa, Jaqueline Gontijo de
Santos, Kênia Valéria dos
Stoianoff, Maria Aparecida de Resende
dc.subject.en.pt_BR.fl_str_mv Candida spp
Orogastric cancer
Virulence
Antifungal
Immune phenotype
topic Candida spp
Orogastric cancer
Virulence
Antifungal
Immune phenotype
description Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Laboratorio de Micologia. Belo Horizonte, MG, Brasil/Universidade Vale do Rio Doce. Laboratorio de Microbiologia. Governador Valadares, MG, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-08-18T16:05:17Z
dc.date.available.fl_str_mv 2016-08-18T16:05:17Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUSA, Lourimar Viana Nascimento Franco de et al. Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype. BMC Infect Dis., v. 16, art. 86, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15309
dc.identifier.issn.pt_BR.fl_str_mv 1471-2334
dc.identifier.doi.none.fl_str_mv 10.1186/s12879-016-1431-4
identifier_str_mv SOUSA, Lourimar Viana Nascimento Franco de et al. Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype. BMC Infect Dis., v. 16, art. 86, 2016.
1471-2334
10.1186/s12879-016-1431-4
url https://www.arca.fiocruz.br/handle/icict/15309
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
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