Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/29069 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. |
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Trindade, Gisela F.Marchevsky, Renato S.Fillipis, Ana Maria Bispo deNogueira, Rita M. R.Bonaldo, Myrna C.Acero, Pedro C.Caride, ElenaFreire, Marcos S.Galler, Ricardo2018-09-26T14:31:42Z2018-09-26T14:31:42Z2007TRINDADE, Gisela F; et al. Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys. Anais da Academia Brasileira de Ciências, v.80, n.2, p.311-321, 2008.0001-3765https://www.arca.fiocruz.br/handle/icict/29069engAcademia Brasileira de Ciênciasfebre amareladenguevacinaReação em Cadeia da Polimerase em Tempo RealReplicaçãoAtenuaçãoyellow feverdenguevaccineattenuationreplicationreal-time PCRLimited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.For the development of safe live attenuated flavivirus vaccines one of the main properties to be established is viral replication. We have used real-time reverse transcriptase-polymerase chain reaction and virus titration by plaque assay to determine the replication of yellow fever 17DD virus (YFV 17DD) and recombinant yellow fever 17D viruses expressing envelope proteins of dengue virus serotypes 2 and 4 (17D-DENV-2 and 17D-DENV-4). Serum samples from rhesus monkeys inoculated with YFV 17DD and 17D-DENV chimeras by intracerebral or subcutaneous route were used to determine and compare the viremia induced by these viruses. Viral load quantification in samples from monkeys inoculated by either route with YFV 17DD virus suggested a restricted capability of the virus to replicate reaching not more than 2.0 log10 PFU mL−1 or 3.29 log10 copies mL−1. Recombinant 17D-dengue viruses were shown by plaquing and real-time PCR to be as attenuated as YF 17DD virus with the highest mean peak titer of 1.97 log10 PFU mL−1 or 3.53 log10 copies mL−1. These data serve as a comparative basis for the characterization of other 17D-based live attenuated candidate vaccines against other diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29069/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALgisela_trindade_etal_IOC_2008.pdfgisela_trindade_etal_IOC_2008.pdfapplication/pdf164418https://www.arca.fiocruz.br/bitstream/icict/29069/2/gisela_trindade_etal_IOC_2008.pdf25f0335e5f1b3df9d8dfc897c0d102e4MD52TEXTgisela_trindade_etal_IOC_2008.pdf.txtgisela_trindade_etal_IOC_2008.pdf.txtExtracted texttext/plain37861https://www.arca.fiocruz.br/bitstream/icict/29069/3/gisela_trindade_etal_IOC_2008.pdf.txt236c231dd8b77f03fb6943d0e1eb7e7fMD53icict/290692018-09-27 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| dc.title.pt_BR.fl_str_mv |
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys |
| title |
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys |
| spellingShingle |
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys Trindade, Gisela F. febre amarela dengue vacina Reação em Cadeia da Polimerase em Tempo Real Replicação Atenuação yellow fever dengue vaccine attenuation replication real-time PCR |
| title_short |
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys |
| title_full |
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys |
| title_fullStr |
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys |
| title_full_unstemmed |
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys |
| title_sort |
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys |
| author |
Trindade, Gisela F. |
| author_facet |
Trindade, Gisela F. Marchevsky, Renato S. Fillipis, Ana Maria Bispo de Nogueira, Rita M. R. Bonaldo, Myrna C. Acero, Pedro C. Caride, Elena Freire, Marcos S. Galler, Ricardo |
| author_role |
author |
| author2 |
Marchevsky, Renato S. Fillipis, Ana Maria Bispo de Nogueira, Rita M. R. Bonaldo, Myrna C. Acero, Pedro C. Caride, Elena Freire, Marcos S. Galler, Ricardo |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Trindade, Gisela F. Marchevsky, Renato S. Fillipis, Ana Maria Bispo de Nogueira, Rita M. R. Bonaldo, Myrna C. Acero, Pedro C. Caride, Elena Freire, Marcos S. Galler, Ricardo |
| dc.subject.other.pt_BR.fl_str_mv |
febre amarela dengue vacina Reação em Cadeia da Polimerase em Tempo Real Replicação Atenuação |
| topic |
febre amarela dengue vacina Reação em Cadeia da Polimerase em Tempo Real Replicação Atenuação yellow fever dengue vaccine attenuation replication real-time PCR |
| dc.subject.en.pt_BR.fl_str_mv |
yellow fever dengue vaccine attenuation replication real-time PCR |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. |
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2007 |
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2007 |
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2018-09-26T14:31:42Z |
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2018-09-26T14:31:42Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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TRINDADE, Gisela F; et al. Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys. Anais da Academia Brasileira de Ciências, v.80, n.2, p.311-321, 2008. |
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https://www.arca.fiocruz.br/handle/icict/29069 |
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0001-3765 |
| identifier_str_mv |
TRINDADE, Gisela F; et al. Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys. Anais da Academia Brasileira de Ciências, v.80, n.2, p.311-321, 2008. 0001-3765 |
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Academia Brasileira de Ciências |
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Academia Brasileira de Ciências |
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