An ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazon

Detalhes bibliográficos
Autor(a) principal: Barbosa, Laila R. A.
Data de Publicação: 2020
Outros Autores: Silva, Emanuelle L., Almeida, Anne C. G., Salazar, Yanka E. A. R., Siqueira, André M., Alecrim, Maria das Graças Costa, Vieira, José Luiz Fernandes, Bassat, Quique, Lacerda, Marcus V. G., Monteiro, Wuelton M., Melo, Gisely C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46026
Resumo: Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.
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spelling Barbosa, Laila R. A.Silva, Emanuelle L.Almeida, Anne C. G.Salazar, Yanka E. A. R.Siqueira, André M.Alecrim, Maria das Graças CostaVieira, José Luiz FernandesBassat, QuiqueLacerda, Marcus V. G.Monteiro, Wuelton M.Melo, Gisely C.2021-02-09T22:12:56Z2021-02-09T22:12:56Z2020BARBOSA, Laila R. A. et al. An Ultra-Sensitive Technique: Using Pv-mtCOX1 qPCR to Detect Early Recurrences of Plasmodium vivax in Patients in the Brazilian Amazon. Pathogens, v. 10, n. 1, p. 1-9, 2020.2076-0817https://www.arca.fiocruz.br/handle/icict/4602610.3390/pathogens10010019engMDPIAn ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil / Universidade Nilton Lins. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil / Faculdade Metropolitana de Manaus. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Universidade Federal do Pará. Instituto de Ciências da Saúde. Belém, PA, Brasil.ISGlobal/Universitat de Barcelona. Hospital Clínic. Barcelona, Spain / Fundação Clínic per la Recerca Biomédica. Centro de Investigação em Saúde de Manhiça. Maputo, Mozambique / Catalan Institution for Research and Advanced Studies. Barcelona, Spain / University of Barcelona. Hospital Sant Joan de Déu. Pediatrics Department. Pediatric Infectious Diseases Unit. Barcelona, Spain / Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública. Madrid, Spain.Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Background: Early recurrence of Plasmodium vivax is a challenge for malaria control in the field, particularly because this species is associated with lower parasitemia, which hinders diagnosis and monitoring through blood smear testing. Early recurrences, defined as the persistence of parasites in the peripheral blood despite adequate drug dosages, may arise from resistance to chloroquine. The objective of the study was to estimate early recurrence of P. vivax in the Brazilian Amazon by using a highly-sensitive detection method, in this case, PCR. Methods: An ultra-sensitive qPCR that targeted mitochondrial DNA was used to compare a standard qPCR that targeted 18S rDNA to detect early recurrence of P. vivax in very low densities in samples from patients treated with chloroquine. Results: Out of a total of 312 cases, 29 samples (9.3%) were characterized as recurrences, from which 3.2% (10/312) were only detected through ultra-sensitive qPCR testing. Conclusions: Studies that report the detection of P. vivax early recurrences using light microscopy may severely underestimate their true incidence.Chloroquine resistanceMalaria diagnosisMitochondrial DNASubmicroscopic infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/46026/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALAn Ultra-Sensitive_Andre_Siqueira_etal_INI_2020.pdfAn Ultra-Sensitive_Andre_Siqueira_etal_INI_2020.pdfapplication/pdf1470575https://www.arca.fiocruz.br/bitstream/icict/46026/2/An%20Ultra-Sensitive_Andre_Siqueira_etal_INI_2020.pdff7ef4d29bf184b265e4737699a3f8775MD52TEXTAn Ultra-Sensitive_Andre_Siqueira_etal_INI_2020.pdf.txtAn Ultra-Sensitive_Andre_Siqueira_etal_INI_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv An ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazon
title An ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazon
spellingShingle An ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazon
Barbosa, Laila R. A.
Chloroquine resistance
Malaria diagnosis
Mitochondrial DNA
Submicroscopic infection.
title_short An ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazon
title_full An ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazon
title_fullStr An ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazon
title_full_unstemmed An ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazon
title_sort An ultra-sensitive technique: Using pv-mtcox1 qpcr to detect early recurrences of plasmodium vivax in patients in the brazilian amazon
author Barbosa, Laila R. A.
author_facet Barbosa, Laila R. A.
Silva, Emanuelle L.
Almeida, Anne C. G.
Salazar, Yanka E. A. R.
Siqueira, André M.
Alecrim, Maria das Graças Costa
Vieira, José Luiz Fernandes
Bassat, Quique
Lacerda, Marcus V. G.
Monteiro, Wuelton M.
Melo, Gisely C.
author_role author
author2 Silva, Emanuelle L.
Almeida, Anne C. G.
Salazar, Yanka E. A. R.
Siqueira, André M.
Alecrim, Maria das Graças Costa
Vieira, José Luiz Fernandes
Bassat, Quique
Lacerda, Marcus V. G.
Monteiro, Wuelton M.
Melo, Gisely C.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Laila R. A.
Silva, Emanuelle L.
Almeida, Anne C. G.
Salazar, Yanka E. A. R.
Siqueira, André M.
Alecrim, Maria das Graças Costa
Vieira, José Luiz Fernandes
Bassat, Quique
Lacerda, Marcus V. G.
Monteiro, Wuelton M.
Melo, Gisely C.
dc.subject.en.pt_BR.fl_str_mv Chloroquine resistance
Malaria diagnosis
Mitochondrial DNA
Submicroscopic infection.
topic Chloroquine resistance
Malaria diagnosis
Mitochondrial DNA
Submicroscopic infection.
description Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-02-09T22:12:56Z
dc.date.available.fl_str_mv 2021-02-09T22:12:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, Laila R. A. et al. An Ultra-Sensitive Technique: Using Pv-mtCOX1 qPCR to Detect Early Recurrences of Plasmodium vivax in Patients in the Brazilian Amazon. Pathogens, v. 10, n. 1, p. 1-9, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46026
dc.identifier.issn.pt_BR.fl_str_mv 2076-0817
dc.identifier.doi.none.fl_str_mv 10.3390/pathogens10010019
identifier_str_mv BARBOSA, Laila R. A. et al. An Ultra-Sensitive Technique: Using Pv-mtCOX1 qPCR to Detect Early Recurrences of Plasmodium vivax in Patients in the Brazilian Amazon. Pathogens, v. 10, n. 1, p. 1-9, 2020.
2076-0817
10.3390/pathogens10010019
url https://www.arca.fiocruz.br/handle/icict/46026
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
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