Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference

Detalhes bibliográficos
Autor(a) principal: Gentile, Carla
Data de Publicação: 2013
Outros Autores: Rivas, Gustavo Bueno da S., Lima, José B. P., Bruno, Rafaela Vieira, Peixoto, Alexandre Afrânio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11565
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Gentile, CarlaRivas, Gustavo Bueno da S.Lima, José B. P.Bruno, Rafaela VieiraPeixoto, Alexandre Afrânio2015-08-19T13:49:33Z2015-08-19T13:49:33Z2013GENTILE, Carla; et al. Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference. Mem Inst Oswaldo Cruz, v.108, Suppl. I, p.80-87, 2013.1678-8060https://www.arca.fiocruz.br/handle/icict/1156510.1590/0074-0276130471engFundação Oswaldo CruzCircadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interferenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. 3Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, BraFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, BraMosquitoes are the culprits of some of the most important vector borne diseases. A species’ potential as a vector is directly dependent on their pattern of behaviour, which is known to change according to the female’s physiological status such as whether the female is virgin/mated and unfed/blood-fed. However, the molecular mechanism triggered by and/or responsible for such modulations in behaviour is poorly understood. Clock genes are known to be responsible for the control of circadian behaviour in several species. Here we investigate the impact mating and blood-feeding have upon the expression of these genes in the mosquito Aedes aegypti. We show that blood intake, but not insemination, is responsible for the down-regulation of clock genes. Using RNA interference, we observe a slight reduction in the evening activity peak in the fourth day after dstim injection. These data suggest that, as in Drosophila, clock gene expression, circadian behaviour and environmental light regimens are interconnected in Ae. aegypti.Clock genesBlood feedingInseminationRNAiBehaviourAedes aegyptiAedesInseminaçãoComportametoCulicidaeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11565/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALsamira_ehlers_etal_IOC_2013.pdfapplication/pdf287581https://www.arca.fiocruz.br/bitstream/icict/11565/2/samira_ehlers_etal_IOC_2013.pdf8e5f11fa5eb89fad2b4b95b764ca65faMD52TEXTsamira_ehlers_etal_IOC_2013.pdf.txtsamira_ehlers_etal_IOC_2013.pdf.txtExtracted texttext/plain36538https://www.arca.fiocruz.br/bitstream/icict/11565/3/samira_ehlers_etal_IOC_2013.pdf.txtce3f5ea092f8805f58bf151df0d40656MD53icict/115652022-06-24 12:18:52.977oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:18:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
title Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
spellingShingle Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
Gentile, Carla
Clock genes
Blood feeding
Insemination
RNAi
Behaviour
Aedes aegypti
Aedes
Inseminação
Comportameto
Culicidae
title_short Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
title_full Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
title_fullStr Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
title_full_unstemmed Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
title_sort Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
author Gentile, Carla
author_facet Gentile, Carla
Rivas, Gustavo Bueno da S.
Lima, José B. P.
Bruno, Rafaela Vieira
Peixoto, Alexandre Afrânio
author_role author
author2 Rivas, Gustavo Bueno da S.
Lima, José B. P.
Bruno, Rafaela Vieira
Peixoto, Alexandre Afrânio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gentile, Carla
Rivas, Gustavo Bueno da S.
Lima, José B. P.
Bruno, Rafaela Vieira
Peixoto, Alexandre Afrânio
dc.subject.en.pt_BR.fl_str_mv Clock genes
Blood feeding
Insemination
RNAi
Behaviour
Aedes aegypti
topic Clock genes
Blood feeding
Insemination
RNAi
Behaviour
Aedes aegypti
Aedes
Inseminação
Comportameto
Culicidae
dc.subject.decs.pt_BR.fl_str_mv Aedes
Inseminação
Comportameto
Culicidae
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-08-19T13:49:33Z
dc.date.available.fl_str_mv 2015-08-19T13:49:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GENTILE, Carla; et al. Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference. Mem Inst Oswaldo Cruz, v.108, Suppl. I, p.80-87, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11565
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0074-0276130471
identifier_str_mv GENTILE, Carla; et al. Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference. Mem Inst Oswaldo Cruz, v.108, Suppl. I, p.80-87, 2013.
1678-8060
10.1590/0074-0276130471
url https://www.arca.fiocruz.br/handle/icict/11565
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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