Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000900080 |
Resumo: | Mosquitoes are the culprits of some of the most important vector borne diseases. A species’ potential as a vector is directly dependent on their pattern of behaviour, which is known to change according to the female’s physiological status such as whether the female is virgin/mated and unfed/blood-fed. However, the molecular mechanism triggered by and/or responsible for such modulations in behaviour is poorly understood. Clock genes are known to be responsible for the control of circadian behaviour in several species. Here we investigate the impact mating and blood-feeding have upon the expression of these genes in the mosquito Aedes aegypti . We show that blood intake, but not insemination, is responsible for the down-regulation of clock genes. Using RNA interference, we observe a slight reduction in the evening activity peak in the fourth day after dstim injection. These data suggest that, as in Drosophila , clock gene expression, circadian behaviour and environmental light regimens are interconnected in Ae. aegypti . |
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Memórias do Instituto Oswaldo Cruz |
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Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interferenceclock genesblood-feedinginseminationRNAibehaviourAedes aegyptiMosquitoes are the culprits of some of the most important vector borne diseases. A species’ potential as a vector is directly dependent on their pattern of behaviour, which is known to change according to the female’s physiological status such as whether the female is virgin/mated and unfed/blood-fed. However, the molecular mechanism triggered by and/or responsible for such modulations in behaviour is poorly understood. Clock genes are known to be responsible for the control of circadian behaviour in several species. Here we investigate the impact mating and blood-feeding have upon the expression of these genes in the mosquito Aedes aegypti . We show that blood intake, but not insemination, is responsible for the down-regulation of clock genes. Using RNA interference, we observe a slight reduction in the evening activity peak in the fourth day after dstim injection. These data suggest that, as in Drosophila , clock gene expression, circadian behaviour and environmental light regimens are interconnected in Ae. aegypti .Instituto Oswaldo Cruz, Ministério da Saúde2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000900080Memórias do Instituto Oswaldo Cruz v.108 suppl.1 2013reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276130471info:eu-repo/semantics/openAccessGentile,CarlaRivas,Gustavo Bueno da SLima,Jose BPBruno,Rafaela VieiraPeixoto,Alexandre Afranioeng2020-04-25T17:51:38Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:21.459Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference |
title |
Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference |
spellingShingle |
Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference Gentile,Carla clock genes blood-feeding insemination RNAi behaviour Aedes aegypti |
title_short |
Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference |
title_full |
Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference |
title_fullStr |
Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference |
title_full_unstemmed |
Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference |
title_sort |
Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference |
author |
Gentile,Carla |
author_facet |
Gentile,Carla Rivas,Gustavo Bueno da S Lima,Jose BP Bruno,Rafaela Vieira Peixoto,Alexandre Afranio |
author_role |
author |
author2 |
Rivas,Gustavo Bueno da S Lima,Jose BP Bruno,Rafaela Vieira Peixoto,Alexandre Afranio |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gentile,Carla Rivas,Gustavo Bueno da S Lima,Jose BP Bruno,Rafaela Vieira Peixoto,Alexandre Afranio |
dc.subject.por.fl_str_mv |
clock genes blood-feeding insemination RNAi behaviour Aedes aegypti |
topic |
clock genes blood-feeding insemination RNAi behaviour Aedes aegypti |
dc.description.none.fl_txt_mv |
Mosquitoes are the culprits of some of the most important vector borne diseases. A species’ potential as a vector is directly dependent on their pattern of behaviour, which is known to change according to the female’s physiological status such as whether the female is virgin/mated and unfed/blood-fed. However, the molecular mechanism triggered by and/or responsible for such modulations in behaviour is poorly understood. Clock genes are known to be responsible for the control of circadian behaviour in several species. Here we investigate the impact mating and blood-feeding have upon the expression of these genes in the mosquito Aedes aegypti . We show that blood intake, but not insemination, is responsible for the down-regulation of clock genes. Using RNA interference, we observe a slight reduction in the evening activity peak in the fourth day after dstim injection. These data suggest that, as in Drosophila , clock gene expression, circadian behaviour and environmental light regimens are interconnected in Ae. aegypti . |
description |
Mosquitoes are the culprits of some of the most important vector borne diseases. A species’ potential as a vector is directly dependent on their pattern of behaviour, which is known to change according to the female’s physiological status such as whether the female is virgin/mated and unfed/blood-fed. However, the molecular mechanism triggered by and/or responsible for such modulations in behaviour is poorly understood. Clock genes are known to be responsible for the control of circadian behaviour in several species. Here we investigate the impact mating and blood-feeding have upon the expression of these genes in the mosquito Aedes aegypti . We show that blood intake, but not insemination, is responsible for the down-regulation of clock genes. Using RNA interference, we observe a slight reduction in the evening activity peak in the fourth day after dstim injection. These data suggest that, as in Drosophila , clock gene expression, circadian behaviour and environmental light regimens are interconnected in Ae. aegypti . |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000900080 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000900080 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-0276130471 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.108 suppl.1 2013 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937715062243328 |