CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro

Detalhes bibliográficos
Autor(a) principal: Raposo, Jéssica Vasques
Data de Publicação: 2021
Outros Autores: Barros, Luciana Rodrigues Carvalho, Torres, Lauana Ribas, Pinto, Rafaela Barbosa da Silva, Lopes, Amanda de Oliveira, Souza, Elen Mello de, Bonamino, Martin Hernan, Paula, Vanessa Salete de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58279
Resumo: DOI: https://doi.org/10.21203/rs.3.rs-833312/v1
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spelling Raposo, Jéssica VasquesBarros, Luciana Rodrigues CarvalhoTorres, Lauana RibasPinto, Rafaela Barbosa da SilvaLopes, Amanda de OliveiraSouza, Elen Mello deBonamino, Martin HernanPaula, Vanessa Salete de2023-05-10T13:14:18Z2023-05-10T13:14:18Z2021RAPOSO, Jèssica Vasques et al. CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro. Research Square, p. 1 - 10, Oct. 2021.https://www.arca.fiocruz.br/handle/icict/58279DOI: https://doi.org/10.21203/rs.3.rs-833312/v1Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.INCA: Instituto Nacional de CâncerFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.INCA: Instituto Nacional de Câncer,Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.HSV-1 affects approximately 67% of the world population. Here, we sought to use the CRISPR / Cas9 system with the UL39 target, essential for virus replication. The sgRNA sequence was inserted into plasmid (PX459-UL39). Vero cells were transfected with PX459-UL39, and inhibition of viral replication was assessed 24 and 48 hours later using plaque assays and fluorescence and qPCR. Fluorescence analyzes revealed the presence of anti-HSV-1 CRISPR/Cas9 within Vero cells, and qPCR showed that the viral load decreased by> 95% of cells transfected with anti-HSV-1 CRISPR / Cas9. Our data demonstrate the usefulness of the PX459-UL39 to inhibit HSV-1 infection.engResearch SquareHuman alphaherpesvirus 1 (HSV-1)CRISPR-associated (CRISPR/Cas)HSV-1sgRNAFluorescenceCRISPR/Cas9CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/58279/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJessicaRa´poso_etal_IOC_2023.pdfJessicaRa´poso_etal_IOC_2023.pdfapplication/pdf1286303https://www.arca.fiocruz.br/bitstream/icict/58279/2/JessicaRa%c2%b4poso_etal_IOC_2023.pdfc2c630d85ce0ccd9f6cbca7d5310235fMD52icict/582792023-05-10 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dc.title.en_US.fl_str_mv CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
title CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
spellingShingle CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
Raposo, Jéssica Vasques
Human alphaherpesvirus 1 (HSV-1)
CRISPR-associated (CRISPR/Cas)
HSV-1
sgRNA
Fluorescence
CRISPR/Cas9
title_short CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
title_full CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
title_fullStr CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
title_full_unstemmed CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
title_sort CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
author Raposo, Jéssica Vasques
author_facet Raposo, Jéssica Vasques
Barros, Luciana Rodrigues Carvalho
Torres, Lauana Ribas
Pinto, Rafaela Barbosa da Silva
Lopes, Amanda de Oliveira
Souza, Elen Mello de
Bonamino, Martin Hernan
Paula, Vanessa Salete de
author_role author
author2 Barros, Luciana Rodrigues Carvalho
Torres, Lauana Ribas
Pinto, Rafaela Barbosa da Silva
Lopes, Amanda de Oliveira
Souza, Elen Mello de
Bonamino, Martin Hernan
Paula, Vanessa Salete de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Raposo, Jéssica Vasques
Barros, Luciana Rodrigues Carvalho
Torres, Lauana Ribas
Pinto, Rafaela Barbosa da Silva
Lopes, Amanda de Oliveira
Souza, Elen Mello de
Bonamino, Martin Hernan
Paula, Vanessa Salete de
dc.subject.other.en_US.fl_str_mv Human alphaherpesvirus 1 (HSV-1)
CRISPR-associated (CRISPR/Cas)
topic Human alphaherpesvirus 1 (HSV-1)
CRISPR-associated (CRISPR/Cas)
HSV-1
sgRNA
Fluorescence
CRISPR/Cas9
dc.subject.en.en_US.fl_str_mv HSV-1
sgRNA
Fluorescence
CRISPR/Cas9
description DOI: https://doi.org/10.21203/rs.3.rs-833312/v1
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2023-05-10T13:14:18Z
dc.date.available.fl_str_mv 2023-05-10T13:14:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAPOSO, Jèssica Vasques et al. CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro. Research Square, p. 1 - 10, Oct. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58279
identifier_str_mv RAPOSO, Jèssica Vasques et al. CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro. Research Square, p. 1 - 10, Oct. 2021.
url https://www.arca.fiocruz.br/handle/icict/58279
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Research Square
publisher.none.fl_str_mv Research Square
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/58279/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/58279/2/JessicaRa%c2%b4poso_etal_IOC_2023.pdf
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