Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12083 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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Figueira, Cláudio PereiraCarvalhal, Djalma Gomes FerrãoAlmeida, Rafaela AndradeHermida, Micely D' El ReiTouchard, DominiqueRobert, PhillipePierres, AnneBongrand, PierredosSantos, Washington Luis Conrado2015-10-26T14:00:03Z2015-10-26T14:00:03Z2015FIGUEIRA, C. P. et al. Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. Scientific Reports, v. 5, p. 12862, 2015.2045-2322https://www.arca.fiocruz.br/handle/icict/1208310.1038/srep12862engNature Publishing GroupLeishmaniaFagócitosMonócitosInfecçãoFibronectinaParasitaLeishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Universidade do Estado da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilAix-Marseille Université. Parc Scientifique de Luminy. Laboratoire Adhésion Cellulaire et Inflammation. Marseille, FranceAix-Marseille Université. Parc Scientifique de Luminy. Laboratoire Adhésion Cellulaire et Inflammation. Marseille, FranceAix-Marseille Université. Parc Scientifique de Luminy. Laboratoire Adhésion Cellulaire et Inflammation. Marseille, FranceAix-Marseille Université. Parc Scientifique de Luminy. Laboratoire Adhésion Cellulaire et Inflammation. Marseille, FranceFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilContact with Leishmania leads to a decreases in mononuclear phagocyte adherence to connective tissue. In this work, we studied the early stages of bond formation between VLA4 and fibronectin, measured the kinetics of membrane alignment and the monocyte cytoplasm spreading area over a fibronectin-coated surface, and studied the expression of high affinity integrin epitope in uninfected and Leishmania-infected human monocytes. Our results show that the initial VLA4-mediated interaction of Leishmania-infected monocyte with a fibronectin-coated surface is preserved, however, the later stage, leukocyte spreading over the substrate is abrogated in Leishmania-infected cells. The median of spreading area was 72 [55–89] μm2 for uninfected and 41 [34–51] μm2 for Leishmaniainfected monocyte. This cytoplasm spread was inhibited using an anti-VLA4 blocking antibody. After the initial contact with the fibronectrin-coated surface, uninfected monocyte quickly spread the cytoplasm at a 15 μm2 s−1 ratio whilst Leishmania-infected monocytes only made small contacts at a 5.5 μm2 s−1 ratio. The expression of high affinity epitope by VLA4 (from 39 ± 21% to 14 ± 3%); and LFA1 (from 37 ± 32% to 18 ± 16%) molecules was reduced in Leishmania-infected monocytes. These changes in phagocyte function may be important for parasite dissemination and distribution of lesions in leishmaniasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/12083/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALFigueira CP Leishmania infection....pdfFigueira CP Leishmania infection....pdfapplication/pdf1686737https://www.arca.fiocruz.br/bitstream/icict/12083/2/Figueira%20CP%20Leishmania%20infection....pdfa9b8779db2b37722c666e01e3665b747MD52TEXTFigueira CP Leishmania infection....pdf.txtFigueira CP Leishmania infection....pdf.txtExtracted texttext/plain43213https://www.arca.fiocruz.br/bitstream/icict/12083/3/Figueira%20CP%20Leishmania%20infection....pdf.txt5d5bad00e7c510c2becca0d4033a104eMD53icict/120832023-03-15 14:33:09.817oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. |
| title |
Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. |
| spellingShingle |
Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. Figueira, Cláudio Pereira Leishmania Fagócitos Monócitos Infecção Fibronectina Parasita |
| title_short |
Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. |
| title_full |
Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. |
| title_fullStr |
Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. |
| title_full_unstemmed |
Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. |
| title_sort |
Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. |
| author |
Figueira, Cláudio Pereira |
| author_facet |
Figueira, Cláudio Pereira Carvalhal, Djalma Gomes Ferrão Almeida, Rafaela Andrade Hermida, Micely D' El Rei Touchard, Dominique Robert, Phillipe Pierres, Anne Bongrand, Pierre dosSantos, Washington Luis Conrado |
| author_role |
author |
| author2 |
Carvalhal, Djalma Gomes Ferrão Almeida, Rafaela Andrade Hermida, Micely D' El Rei Touchard, Dominique Robert, Phillipe Pierres, Anne Bongrand, Pierre dosSantos, Washington Luis Conrado |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Figueira, Cláudio Pereira Carvalhal, Djalma Gomes Ferrão Almeida, Rafaela Andrade Hermida, Micely D' El Rei Touchard, Dominique Robert, Phillipe Pierres, Anne Bongrand, Pierre dosSantos, Washington Luis Conrado |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania Fagócitos Monócitos Infecção Fibronectina Parasita |
| topic |
Leishmania Fagócitos Monócitos Infecção Fibronectina Parasita |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
| publishDate |
2015 |
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2015-10-26T14:00:03Z |
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2015-10-26T14:00:03Z |
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2015 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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FIGUEIRA, C. P. et al. Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. Scientific Reports, v. 5, p. 12862, 2015. |
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https://www.arca.fiocruz.br/handle/icict/12083 |
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2045-2322 |
| dc.identifier.doi.none.fl_str_mv |
10.1038/srep12862 |
| identifier_str_mv |
FIGUEIRA, C. P. et al. Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. Scientific Reports, v. 5, p. 12862, 2015. 2045-2322 10.1038/srep12862 |
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Nature Publishing Group |
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Nature Publishing Group |
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