Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence

Detalhes bibliográficos
Autor(a) principal: Santos, Théo de Araújo
Data de Publicação: 2014
Outros Autores: Rodríguez, Nilda E, Pontes, Sara Moura, Dixt, Upasna Gaur, Abánades, Daniel Ruiz, Bozza, Patrícia Torres, Wilson, Mary Edithe, Borges, Valeria de Matos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8466
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / University of Iowa and the Iowa City VA Medical Center. Iowa City, Iowa
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spelling Santos, Théo de AraújoRodríguez, Nilda EPontes, Sara MouraDixt, Upasna GaurAbánades, Daniel RuizBozza, Patrícia TorresWilson, Mary EditheBorges, Valeria de Matos2014-09-29T16:39:00Z2014-09-29T16:39:00Z2014SANTOS, T. A. et al. Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence. Journal of Infectious Diseases, jun. 2014.0022-1899https://www.arca.fiocruz.br/handle/icict/846610.1093/infdis/jiu299engOxford University PressRole of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / University of Iowa and the Iowa City VA Medical Center. Iowa City, IowaUniversity of Iowa and the Iowa City VA Medical Center. Iowa City, IowaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilUniversity of Iowa and the Iowa City VA Medical Center. Iowa City, IowaCentro de Investigaciones Biológicas, CSIC. Department of Chemical and Physical Biology. Madrid, SpainFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversity of Iowa and the Iowa City VA Medical Center. Iowa City, IowaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute for Investigation in Immunology, iii-INCT. National Institute of Science and Technology. São Paulo, SP, BrasilLipid bodies (LB; lipid droplets) are cytoplasmic organelles involved in lipid metabolism. Mammalian LBs display an important role in host-pathogen interactions, but the role of parasite LBs in biosynthesis of prostaglandin F2α (PGF2α) has not been investigated. We report herein that LBs increased in abundance during development of Leishmania infantum chagasi to a virulent metacyclic stage, as did the expression of PGF2α synthase (PGFS). The amount of parasite LBs and PGF2α were modulated by exogenous arachidonic acid. During macrophage infection, LBs were restricted to parasites inside the parasitophorous vacuoles (PV). We detected PGF2α receptor (FP) on the Leishmania PV surface. The blockage of FP with AL8810, a selective antagonist, hampered Leishmania infection, whereas the irreversible inhibition of cyclooxygenase with aspirin increased the parasite burden. These data demonstrate novel functions for parasite-derived LBs and PGF2α in the cellular metabolism of Leishmania and its evasion of the host immune response.Leishmania infantum chagasiLipid dropletsProstaglandin F2αPGF2α synthaseMacrophage infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8466/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSantos TA Role....pdfSantos TA Role....pdfapplication/pdf797145https://www.arca.fiocruz.br/bitstream/icict/8466/2/Santos%20TA%20Role....pdff1a53c60daadd7bee2a4bf3a5e90fd71MD52TEXTSantos TA Role....pdf.txtSantos TA Role....pdf.txtExtracted texttext/plain43968https://www.arca.fiocruz.br/bitstream/icict/8466/3/Santos%20TA%20Role....pdf.txtb8db70de7a0f3baa04a50548c245f733MD53icict/84662023-09-14 15:53:26.491oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T18:53:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
title Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
spellingShingle Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
Santos, Théo de Araújo
Leishmania infantum chagasi
Lipid droplets
Prostaglandin F2α
PGF2α synthase
Macrophage infection
title_short Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
title_full Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
title_fullStr Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
title_full_unstemmed Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
title_sort Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
author Santos, Théo de Araújo
author_facet Santos, Théo de Araújo
Rodríguez, Nilda E
Pontes, Sara Moura
Dixt, Upasna Gaur
Abánades, Daniel Ruiz
Bozza, Patrícia Torres
Wilson, Mary Edithe
Borges, Valeria de Matos
author_role author
author2 Rodríguez, Nilda E
Pontes, Sara Moura
Dixt, Upasna Gaur
Abánades, Daniel Ruiz
Bozza, Patrícia Torres
Wilson, Mary Edithe
Borges, Valeria de Matos
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Théo de Araújo
Rodríguez, Nilda E
Pontes, Sara Moura
Dixt, Upasna Gaur
Abánades, Daniel Ruiz
Bozza, Patrícia Torres
Wilson, Mary Edithe
Borges, Valeria de Matos
dc.subject.en.pt_BR.fl_str_mv Leishmania infantum chagasi
Lipid droplets
Prostaglandin F2α
PGF2α synthase
Macrophage infection
topic Leishmania infantum chagasi
Lipid droplets
Prostaglandin F2α
PGF2α synthase
Macrophage infection
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / University of Iowa and the Iowa City VA Medical Center. Iowa City, Iowa
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-09-29T16:39:00Z
dc.date.available.fl_str_mv 2014-09-29T16:39:00Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, T. A. et al. Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence. Journal of Infectious Diseases, jun. 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8466
dc.identifier.issn.none.fl_str_mv 0022-1899
dc.identifier.doi.none.fl_str_mv 10.1093/infdis/jiu299
identifier_str_mv SANTOS, T. A. et al. Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence. Journal of Infectious Diseases, jun. 2014.
0022-1899
10.1093/infdis/jiu299
url https://www.arca.fiocruz.br/handle/icict/8466
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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