Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay

Detalhes bibliográficos
Autor(a) principal: Souza, Milena Lázaro de
Data de Publicação: 2018
Outros Autores: Matte, Christine, Lima, Jonilson Berlink, Duque, Guillermo Arango, Carvalho, Graziele Quintela de, Vivarini, Áislan de Carvalho, Pontes, Sara Moura, Figueira, Cláudio Pereira, Santos, Flávio Henrique de Jesus, Lopes, Ulisses Gazos, Farias, Leonardo Paiva, Santos, Théo de Araújo, Descoteaux, Albert, Borges, Valéria de Matos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27542
Resumo: Brazilian National Research Council (CNPq), (Grant number: 400135/2014-0 to VB), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) (Grant number: 04/2015 to VB), Science without Borders (SwB) (to VB and AD) and the Canadian Institutes of Health Research (CIHR) (grant MOP-125990 to AD). VB and UG are sênior investigator funded by CNPq.
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spelling Souza, Milena Lázaro deMatte, ChristineLima, Jonilson BerlinkDuque, Guillermo ArangoCarvalho, Graziele Quintela deVivarini, Áislan de CarvalhoPontes, Sara MouraFigueira, Cláudio PereiraSantos, Flávio Henrique de JesusLopes, Ulisses GazosFarias, Leonardo PaivaSantos, Théo de AraújoDescoteaux, AlbertBorges, Valéria de Matos2018-07-16T17:23:01Z2018-07-16T17:23:01Z2018SOUZA, M. L. et al. Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay. Frontiers in Microbiology, v. 9, art 626, 2018.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/2754210.3389/fmicb.2018.00626Brazilian National Research Council (CNPq), (Grant number: 400135/2014-0 to VB), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) (Grant number: 04/2015 to VB), Science without Borders (SwB) (to VB and AD) and the Canadian Institutes of Health Research (CIHR) (grant MOP-125990 to AD). VB and UG are sênior investigator funded by CNPq.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Department of Legal Medicine. Salvador, BA, BrasilInstitut National de la Recherche Scientifique. Institut Armand-Frappier. Laval, QC, CanadaFederal University of Western. Center of Biological Sciences and Health. Barreiras, BA, BrasilInstitut National de la Recherche Scientifique. Institut Armand-Frappier. Laval, QC, CanadaFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Department of Legal Medicine. Salvador, BA, Brasil / Instituto Federal de Educação, Ciência e Tecnologia Baiano. Alagoinhas, BA, BrasilUniversidade Federal do Rio de Janeiro. Carlos Chagas Filho Biophysics Institute. Center of Health Science. Laboratory of Molecular Parasitology. Rio de Janeiro, RJ, BrasilFederal University of Bahia. Department of Legal Medicine. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Department of Legal Medicine. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Carlos Chagas Filho Biophysics Institute. Center of Health Science. Laboratory of Molecular Parasitology. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, BrasilFederal University of Western. Center of Biological Sciences and Health. Barreiras, BA, BrasilInstitut National de la Recherche Scientifique. Institut Armand-Frappier. Laval, QC, CanadaFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Department of Legal Medicine. Salvador, BA, BrasilLipophosphoglycan (LPG) is the major surface glycoconjugate of metacyclic Leishmania promastigotes and is associated with virulence in various species of this parasite. Here, we generated a LPG-deficient mutant of Leishmania infantum, the foremost etiologic agent of visceral leishmaniasis in Brazil. The L. infantum LPG-deficient mutant (Δlpg1) was obtained by homologous recombination and complemented via episomal expression of LPG1 (Δlpg1 + LPG1). Deletion of LPG1 had no observable effect on parasite morphology or on the presence of subcellular organelles, such as lipid droplets. While both wild-type and add-back parasites reached late phase in axenic cultures, the growth of Δlpg1 parasites was delayed. Additionally, the deletion of LPG1 impaired the outcome of infection in murine bone marrow-derived macrophages. Although no significant differences were observed in parasite load after 4 h of infection, survival of Δlpg1 parasites was significantly reduced at 72 h post-infection. Interestingly, L. infantum LPG-deficient mutants induced a strong NF-κB-dependent activation of the inducible nitric oxide synthase (iNOS) promoter compared to wild type and Δlpg1 + LPG1 parasites. In conclusion, the L. infantum Δlpg1 mutant constitutes a powerful tool to investigate the role(s) played by LPG in host cell-parasite interactions.engFrontiers MediaLipofosfoglicanoLeishmania infantumDirecionamento de genesGotículas lipídicasMacrófagosLipophosphoglycanLeishmania infantumGene targetingLipid dropletsMacrophageLeishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27542/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSouza ML Leishmania infantum Lipophosphoglycan....pdfSouza ML Leishmania infantum Lipophosphoglycan....pdfapplication/pdf1759724https://www.arca.fiocruz.br/bitstream/icict/27542/2/Souza%20ML%20Leishmania%20infantum%20Lipophosphoglycan....pdfa46e8f5ca78617d7290da8e82897a6bdMD52TEXTSouza ML Leishmania infantum Lipophosphoglycan....pdf.txtSouza ML Leishmania infantum Lipophosphoglycan....pdf.txtExtracted texttext/plain48649https://www.arca.fiocruz.br/bitstream/icict/27542/3/Souza%20ML%20Leishmania%20infantum%20Lipophosphoglycan....pdf.txt5e185aefff2ee0f2e27e9b2540db4bd3MD53icict/275422023-03-15 14:34:07.119oai:www.arca.fiocruz.br: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ório 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dc.title.pt_BR.fl_str_mv Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay
title Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay
spellingShingle Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay
Souza, Milena Lázaro de
Lipofosfoglicano
Leishmania infantum
Direcionamento de genes
Gotículas lipídicas
Macrófagos
Lipophosphoglycan
Leishmania infantum
Gene targeting
Lipid droplets
Macrophage
title_short Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay
title_full Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay
title_fullStr Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay
title_full_unstemmed Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay
title_sort Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay
author Souza, Milena Lázaro de
author_facet Souza, Milena Lázaro de
Matte, Christine
Lima, Jonilson Berlink
Duque, Guillermo Arango
Carvalho, Graziele Quintela de
Vivarini, Áislan de Carvalho
Pontes, Sara Moura
Figueira, Cláudio Pereira
Santos, Flávio Henrique de Jesus
Lopes, Ulisses Gazos
Farias, Leonardo Paiva
Santos, Théo de Araújo
Descoteaux, Albert
Borges, Valéria de Matos
author_role author
author2 Matte, Christine
Lima, Jonilson Berlink
Duque, Guillermo Arango
Carvalho, Graziele Quintela de
Vivarini, Áislan de Carvalho
Pontes, Sara Moura
Figueira, Cláudio Pereira
Santos, Flávio Henrique de Jesus
Lopes, Ulisses Gazos
Farias, Leonardo Paiva
Santos, Théo de Araújo
Descoteaux, Albert
Borges, Valéria de Matos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Milena Lázaro de
Matte, Christine
Lima, Jonilson Berlink
Duque, Guillermo Arango
Carvalho, Graziele Quintela de
Vivarini, Áislan de Carvalho
Pontes, Sara Moura
Figueira, Cláudio Pereira
Santos, Flávio Henrique de Jesus
Lopes, Ulisses Gazos
Farias, Leonardo Paiva
Santos, Théo de Araújo
Descoteaux, Albert
Borges, Valéria de Matos
dc.subject.other.pt_BR.fl_str_mv Lipofosfoglicano
Leishmania infantum
Direcionamento de genes
Gotículas lipídicas
Macrófagos
topic Lipofosfoglicano
Leishmania infantum
Direcionamento de genes
Gotículas lipídicas
Macrófagos
Lipophosphoglycan
Leishmania infantum
Gene targeting
Lipid droplets
Macrophage
dc.subject.en.pt_BR.fl_str_mv Lipophosphoglycan
Leishmania infantum
Gene targeting
Lipid droplets
Macrophage
description Brazilian National Research Council (CNPq), (Grant number: 400135/2014-0 to VB), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) (Grant number: 04/2015 to VB), Science without Borders (SwB) (to VB and AD) and the Canadian Institutes of Health Research (CIHR) (grant MOP-125990 to AD). VB and UG are sênior investigator funded by CNPq.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-07-16T17:23:01Z
dc.date.available.fl_str_mv 2018-07-16T17:23:01Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, M. L. et al. Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay. Frontiers in Microbiology, v. 9, art 626, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27542
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2018.00626
identifier_str_mv SOUZA, M. L. et al. Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay. Frontiers in Microbiology, v. 9, art 626, 2018.
1664-302X
10.3389/fmicb.2018.00626
url https://www.arca.fiocruz.br/handle/icict/27542
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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