A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans

• Autor(a) principal: Higa, Bárbara
• Data de Publicação: 2022
• Outros Autores: Cintra, Bianca Souza, Álvarez, Carmen Magaly, Ribeiro, Arthur Barcelos, Ferreira, Jair Camargo, Tavares, Denise Crispim, Enriquez, Vanessa, Martinez, Luis R., Pires, Regina Helena
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/4036
• topic: Biofilms; Candida; Oral candidiasis; Ozone; Streptococcus mutans; Sunflower oil; CNPQ::CIENCIAS DA SAUDE::SAUDE COLETIVA

This study explores the growth of bacterial, fungal, and interkingdom biofilms under aerobiosis or microaerobic conditions and the effect of ozonated sunflower oil on these biofilms. Candida species and Streptococcus mutans were used to study this interaction due to their importance in oral health and disease as these microorganisms display a synergistic relationship that manifests in the onset of caries and tooth decay. Biofilms were developed in a 96-well microtiter plate at 37ºC for 24 h, under aerobiosis or microaerobic conditions, and treated with ozonated oil for 5 to 120 min. All the microorganisms formed biofilms in both oxygenation conditions. Scanning electron microscopy was used to visualize biofilm morphology. Rodent experiments were performed to verify the oil-related toxicity and its efficacy in oral candidiasis. The growth of all Candida species was increased when co-cultured with S. mutans, whilst the growth of bacterium was greater only when co-cultured with C. krusei and C. orthopsilosis under aerobiosis and microaerobic conditions, respectively. Regardless of the oxygenation condition, ozonated oil significantly reduced the viability of all the tested biofilms and infected mice, showing remarkable microbicidal activity as corroborated with confocal microscopy and minimal toxicity. Thus, ozonated oil therapy can be explored as a strategy to control diseases associated with these biofilms especially in the oral cavity.