Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório do Centro Universitário Braz Cubas |
Texto Completo: | https://repositorio.cruzeirodosul.edu.br/handle/123456789/2177 |
Resumo: | Bisphosphonate-related osteonecrosis of the jaws (BRONJ) is a serious complication associated with dental intervention and the long-term use of bisphosphonates, a class of drugs used to treat bone pathologies, such as osteoporosis and bone metastases. The purpose of this study is to evaluate the effectiveness of substances in preventing BRONJ. Thirty-six (n = 36) Wistar rats were used, females (n = 18) and males (n = 18). All rats received a weekly intravenous injection of 0.3 mL via a caudal vein of zoledronate (80 μg / kg body weight in PBS) for 8 weeks. Then, the animals underwent surgery to remove the first and second right and left mandibular molars to induce osteonecrosis. Males and females were equally distributed according to the substance in two large groups: biochelating biomaterial (n = 12), in a split-mouth study, and association of pentoxifylline and tocopherol (PENTO) (n = 12) in a case-control study. The bio-chelating group received a local application of 17% EDTA on the right side after extractions at the time of surgery while the left side received irrigation with saline solution. Twelve animals in the PENTO group received a combined suspension of the two substances (5.71 mg pentoxifylline and 7.14 IU tocopherol) via gavage once daily for 6 weeks. Twelve animals were allocated to a control group for the PENTO that received weekly intraperitoneal saline applications, once a week for 6 weeks. Clinical assessments were made by assessing the presence of the following characteristics: bone exposure, inflammation, suppuration, bone sequestration and epithelialization. In addition, the mesio-distal distances as well as the buccal-lingual bone exposure were obtained for each animal. The data were submitted to statistical analysis with a significance level of 0.05. It was possible to observe that in the bio-chelating biomaterial group there was no statistically significant difference in relation to the clinical data for bone exposure, bone sequestration, suppuration, inflammation and epithelialization (p> 0.05). Regarding the size of bone exposure, there was no statistically significant difference between the right and left sides (p = 0.197). The PENTO group demonstrated a lower incidence of bone sequestration when compared to the control (p = 0.006), no other statistically significant difference was obtained in relation to the others observed clinical data (p> 0.05). The PENTO group showed even less bone exposure on the buccal-lingual aspect (p = 0.041) when compared to the control. It is concluded that the PENTO group had more favorable clinical results related to BRONJ when compared to the control. |
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Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in ratsOdontologiaOsteonecroseDifosfonatosPentoxifilinaTocoferóisCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIABisphosphonate-related osteonecrosis of the jaws (BRONJ) is a serious complication associated with dental intervention and the long-term use of bisphosphonates, a class of drugs used to treat bone pathologies, such as osteoporosis and bone metastases. The purpose of this study is to evaluate the effectiveness of substances in preventing BRONJ. Thirty-six (n = 36) Wistar rats were used, females (n = 18) and males (n = 18). All rats received a weekly intravenous injection of 0.3 mL via a caudal vein of zoledronate (80 μg / kg body weight in PBS) for 8 weeks. Then, the animals underwent surgery to remove the first and second right and left mandibular molars to induce osteonecrosis. Males and females were equally distributed according to the substance in two large groups: biochelating biomaterial (n = 12), in a split-mouth study, and association of pentoxifylline and tocopherol (PENTO) (n = 12) in a case-control study. The bio-chelating group received a local application of 17% EDTA on the right side after extractions at the time of surgery while the left side received irrigation with saline solution. Twelve animals in the PENTO group received a combined suspension of the two substances (5.71 mg pentoxifylline and 7.14 IU tocopherol) via gavage once daily for 6 weeks. Twelve animals were allocated to a control group for the PENTO that received weekly intraperitoneal saline applications, once a week for 6 weeks. Clinical assessments were made by assessing the presence of the following characteristics: bone exposure, inflammation, suppuration, bone sequestration and epithelialization. In addition, the mesio-distal distances as well as the buccal-lingual bone exposure were obtained for each animal. The data were submitted to statistical analysis with a significance level of 0.05. It was possible to observe that in the bio-chelating biomaterial group there was no statistically significant difference in relation to the clinical data for bone exposure, bone sequestration, suppuration, inflammation and epithelialization (p> 0.05). Regarding the size of bone exposure, there was no statistically significant difference between the right and left sides (p = 0.197). The PENTO group demonstrated a lower incidence of bone sequestration when compared to the control (p = 0.006), no other statistically significant difference was obtained in relation to the others observed clinical data (p> 0.05). The PENTO group showed even less bone exposure on the buccal-lingual aspect (p = 0.041) when compared to the control. It is concluded that the PENTO group had more favorable clinical results related to BRONJ when compared to the control.A osteonecrose dos maxilares relacionada aos bisfosfonatos (BRONJ) é uma complicação grave associada à intervenção odontológica e ao uso a longo prazo de bifosfonatos, uma classe de medicamentos utilizados para o tratamento de patologias ósseas, tais como osteoporose e metástases ósseas. O objetivo deste estudo é avaliar a efetividade de substâncias na prevenção da BRONJ após exodontia. Trinta e seis (n = 36) ratos Wistar foram utilizados, fêmeas (n = 18) e machos (n = 18). Para a indução da osteonecrose, todos os ratos receberam uma injeção intravenosa semanal de 0,3 mL via veia caudal de zoledronato (80 μg/kg de peso corporal em PBS) por 8 semanas. Em seguida, os animais foram submetidos à cirurgia para remoção dos primeiros molares inferiores direito e esquerdo, e na semana seguinte remoção dos segundos molares inferiores direito e esquerdo. Os machos e fêmeas foram igualmente distribuídos de acordo com a substância em dois grandes grupos: biomaterial bioquelante (n = 12), em um estudo de boca dividida e associação de pentoxifilina e tocoferol (PENTO) (n = 12) em um estudo de casocontrole. O grupo bioquelante recebeu aplicação local de EDTA a 17% no lado direito após as exodontias no momento da cirurgia enquanto o lado esquerdo recebeu irrigação com solução salina. Doze animais do grupo PENTO receberam uma suspensão combinada das duas substâncias (5,71 mg pentoxifilina e 7,14 UI de tocoferol) via gavagem uma vez ao dia por 6 semanas. Doze animais foram alocados em um grupo controle para o PENTO que recebeu aplicações semanais intraperitoneais de solução fisiológica, uma vez na semana por 6 semanas. As avaliações clínicas foram feitas através da avaliação da presença das seguintes características: exposição óssea, inflamação, supuração, sequestro ósseo e epitelização. Além disso, as distâncias mésio-distal bem como vestíbulo-lingual da exposição óssea foram obtidas para cada animal. Os dados foram submetidos a análise estatística com nível de significância de 0,05. Foi possível observar que no grupo bioquelante não houve diferença estatisticamente significante em relação aos dados clínicos para exposição óssea, sequestro ósseo, supuração, inflamação e epitelização (p > 0,05). Em relação ao tamanho da exposição óssea, não houve diferença estatisticamente significativa entre os lados direito e esquerdo (p = 0,197). O grupo PENTO demonstrou menor incidência de sequestro ósseo quando comparado ao controle (p = 0,006), nenhuma outra diferença estatisticamente significante foi obtida em relação aos outros dados clínicos observados (p > 0,05). O grupo PENTO apresentou ainda menor exposição óssea na face vestíbulolingual (p = 0,041) quando comparado ao controle. Conclui-se que o grupo PENTO apresentou resultados clinicamente mais favoráveis relacionadas a BRONJ quando comparado ao controle.Universidade PositivoBrasilPós-GraduaçãoPrograma de Pós-Graduação em Odontologia ClínicaUPDeliberador, Tatiana Mirandahttp://lattes.cnpq.br/4688659299176448Elsalanty, MohammedScariot, Rafaelahttp://lattes.cnpq.br/8726711027143249Weiss, Suyany Gabriely2021-05-14T20:23:55Z20202021-05-14T20:23:55Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://repositorio.cruzeirodosul.edu.br/handle/123456789/2177porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2021-06-24T12:19:35Zoai:repositorio.cruzeirodosul.edu.br:123456789/2177Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2021-06-24T12:19:35Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false |
dc.title.none.fl_str_mv |
Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in rats |
title |
Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in rats |
spellingShingle |
Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in rats Weiss, Suyany Gabriely Odontologia Osteonecrose Difosfonatos Pentoxifilina Tocoferóis CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in rats |
title_full |
Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in rats |
title_fullStr |
Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in rats |
title_full_unstemmed |
Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in rats |
title_sort |
Prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ): evaluation of the effectiveness of different substances in rats |
author |
Weiss, Suyany Gabriely |
author_facet |
Weiss, Suyany Gabriely |
author_role |
author |
dc.contributor.none.fl_str_mv |
Deliberador, Tatiana Miranda http://lattes.cnpq.br/4688659299176448 Elsalanty, Mohammed Scariot, Rafaela http://lattes.cnpq.br/8726711027143249 |
dc.contributor.author.fl_str_mv |
Weiss, Suyany Gabriely |
dc.subject.por.fl_str_mv |
Odontologia Osteonecrose Difosfonatos Pentoxifilina Tocoferóis CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
topic |
Odontologia Osteonecrose Difosfonatos Pentoxifilina Tocoferóis CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Bisphosphonate-related osteonecrosis of the jaws (BRONJ) is a serious complication associated with dental intervention and the long-term use of bisphosphonates, a class of drugs used to treat bone pathologies, such as osteoporosis and bone metastases. The purpose of this study is to evaluate the effectiveness of substances in preventing BRONJ. Thirty-six (n = 36) Wistar rats were used, females (n = 18) and males (n = 18). All rats received a weekly intravenous injection of 0.3 mL via a caudal vein of zoledronate (80 μg / kg body weight in PBS) for 8 weeks. Then, the animals underwent surgery to remove the first and second right and left mandibular molars to induce osteonecrosis. Males and females were equally distributed according to the substance in two large groups: biochelating biomaterial (n = 12), in a split-mouth study, and association of pentoxifylline and tocopherol (PENTO) (n = 12) in a case-control study. The bio-chelating group received a local application of 17% EDTA on the right side after extractions at the time of surgery while the left side received irrigation with saline solution. Twelve animals in the PENTO group received a combined suspension of the two substances (5.71 mg pentoxifylline and 7.14 IU tocopherol) via gavage once daily for 6 weeks. Twelve animals were allocated to a control group for the PENTO that received weekly intraperitoneal saline applications, once a week for 6 weeks. Clinical assessments were made by assessing the presence of the following characteristics: bone exposure, inflammation, suppuration, bone sequestration and epithelialization. In addition, the mesio-distal distances as well as the buccal-lingual bone exposure were obtained for each animal. The data were submitted to statistical analysis with a significance level of 0.05. It was possible to observe that in the bio-chelating biomaterial group there was no statistically significant difference in relation to the clinical data for bone exposure, bone sequestration, suppuration, inflammation and epithelialization (p> 0.05). Regarding the size of bone exposure, there was no statistically significant difference between the right and left sides (p = 0.197). The PENTO group demonstrated a lower incidence of bone sequestration when compared to the control (p = 0.006), no other statistically significant difference was obtained in relation to the others observed clinical data (p> 0.05). The PENTO group showed even less bone exposure on the buccal-lingual aspect (p = 0.041) when compared to the control. It is concluded that the PENTO group had more favorable clinical results related to BRONJ when compared to the control. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020 2021-05-14T20:23:55Z 2021-05-14T20:23:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2177 |
url |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2177 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Odontologia Clínica UP |
publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Odontologia Clínica UP |
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reponame:Repositório do Centro Universitário Braz Cubas instname:Centro Universitário Braz Cubas (CUB) instacron:CUB |
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Centro Universitário Braz Cubas (CUB) |
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CUB |
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CUB |
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Repositório do Centro Universitário Braz Cubas |
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Repositório do Centro Universitário Braz Cubas |
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Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB) |
repository.mail.fl_str_mv |
bibli@brazcubas.edu.br |
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1798311353749864448 |