Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório do Centro Universitário Braz Cubas |
Texto Completo: | https://repositorio.cruzeirodosul.edu.br/handle/123456789/2269 |
Resumo: | Background: Breast is composed of glandular tissue with complex duct-lobular systems. The ductal system flow out of the breast external surface through the areolo-papillary complex. The rates of infection in elective breast surgeries reach values between 3 and 15%, wich are more than expected for operations considered clean and may be associated with manipulation of the areolo-papillary complex and ducts previously colonized by microorganisms. Then, the goal of this study was to investigate if there is a microbiota in the region behind the areola and nipple, where the breast ducts are located, and if it is equal to the microbiota of the skin and to which antibiotics it is sensitive or resistant. Methods: Cultures of breast tissue samples removed from 49 patients who underwent oncologic, aesthetic and reconstructive breast surgeries. Three fragments of breast tissue were removed, about 1 centimeter in diameter each fragment, one retroareolar, lateral, and medial quadrants. Each tissue fragment were transferred into a sterile vial containing BHI broth, and incubated for 7 days at 37°C and in the cases of turbidity of the medium, result of microorganisms growth, will be done fresh blade and sowing in Petry plate for culture and isolation of the strains, antibiogram and identification of the species in automatic reader. Results: There were microorganisms growth in samples from 10 of the 49 patients (20.4%), and 11 of the 218 fragments sampled (5%). The species found were Staphylococcus epidermidis, Staphylococcus lugdunensis, Staphylococcus hominis, Sphingomonas paucimobilis and Aeromonas salmonicida. The presence of these bacteria in about 20% of them could suggest that breast surgeries should be considered as potentially contaminated surgery, and this may have implications for adverse reactions to mammary prosthesis, and also in the genesis of large cell lymphoma associated with prostheses. |
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Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mamaBiotecnologia industrialInfecçãoContraturaBiofilmesMastectomia segmentarCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALBackground: Breast is composed of glandular tissue with complex duct-lobular systems. The ductal system flow out of the breast external surface through the areolo-papillary complex. The rates of infection in elective breast surgeries reach values between 3 and 15%, wich are more than expected for operations considered clean and may be associated with manipulation of the areolo-papillary complex and ducts previously colonized by microorganisms. Then, the goal of this study was to investigate if there is a microbiota in the region behind the areola and nipple, where the breast ducts are located, and if it is equal to the microbiota of the skin and to which antibiotics it is sensitive or resistant. Methods: Cultures of breast tissue samples removed from 49 patients who underwent oncologic, aesthetic and reconstructive breast surgeries. Three fragments of breast tissue were removed, about 1 centimeter in diameter each fragment, one retroareolar, lateral, and medial quadrants. Each tissue fragment were transferred into a sterile vial containing BHI broth, and incubated for 7 days at 37°C and in the cases of turbidity of the medium, result of microorganisms growth, will be done fresh blade and sowing in Petry plate for culture and isolation of the strains, antibiogram and identification of the species in automatic reader. Results: There were microorganisms growth in samples from 10 of the 49 patients (20.4%), and 11 of the 218 fragments sampled (5%). The species found were Staphylococcus epidermidis, Staphylococcus lugdunensis, Staphylococcus hominis, Sphingomonas paucimobilis and Aeromonas salmonicida. The presence of these bacteria in about 20% of them could suggest that breast surgeries should be considered as potentially contaminated surgery, and this may have implications for adverse reactions to mammary prosthesis, and also in the genesis of large cell lymphoma associated with prostheses.OBJETIVO: A cirurgia da mama é classificada como cirurgia limpa com relação ao potencial de contaminação. As taxas de infecção em cirurgias eletivas de mama estão atingindo valores entre 3 e 15%, acima do esperado para operações consideradas limpas e podem estar associadas à manipulação do complexo aréolopapilar e ductos colonizado por micro-organismos. O objetivo deste estudo é investigar a existência de microbiota autóctone na mama, dividindo a mesma em 3 regiões: central (retro-areolar), quadrante medial, e quadrante lateral, e se existe correlação com as variáveis idade, finalidade da cirurgia, localização do tumor, diabetes melitus, quimioterapia neoadjuvante, radioterapia neoadjuvante, índice de massa corpórea (IMC), menopausa e amamentação. MÉTODO: Foram realizadas culturas de fragmentos de tecidos mamários retirados de 49 pacientes submetidas a cirurgias eletivas da mama (reparadora, estética ou oncológica). Foram removidos fragmentos de tecidos da mama, com cerca de 1 centímetro de diâmetro cada fragmento, sendo eles de tecido retroareolar, glandular medial e glandular lateral. Cada fragmento de tecido foi incubado em caldo BHI durante 7 dias a 37°C e nos casos de turvação do meio, resultado de crescimento de micro-organismos, foram realizados semeadura em placa de Petry para cultura e isolamento das cepas e identificação das espécies em leitor automático. RESULTADOS: Houve crescimento de microorganismos em amostras de 10 das 49 pacientes (20,4%) e 11 dos 218 fragmentos amostrados (5%). As espécies encontradas foram: Staphylococcus epidermidis, Staphylococcus lugdunensis, Staphylococcus hominis, Sphingomonas paucimobilis e Aeromonas salmonicida. CONCLUSÕES: A presença destas bactérias em cerca de 20% delas pode sugerir que as cirurgias mamárias devam ser consideradas como potencialmente contaminadas e isto pode ter implicações para reações adversas às próteses mamárias e também na gênese do linfoma de grandes células associado às próteses.Universidade PositivoBrasilPós-GraduaçãoPrograma de Pós-Graduação em Biotecnologia IndustrialUPUrban, Cicero de Andradehttp://lattes.cnpq.br/0718187625853159Stachon, Henrique Alexandre2021-05-26T13:41:51Z20182021-05-26T13:41:51Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://repositorio.cruzeirodosul.edu.br/handle/123456789/2269porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2021-09-09T20:53:18Zoai:repositorio.cruzeirodosul.edu.br:123456789/2269Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2021-09-09T20:53:18Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false |
dc.title.none.fl_str_mv |
Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama |
title |
Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama |
spellingShingle |
Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama Stachon, Henrique Alexandre Biotecnologia industrial Infecção Contratura Biofilmes Mastectomia segmentar CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama |
title_full |
Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama |
title_fullStr |
Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama |
title_full_unstemmed |
Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama |
title_sort |
Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama |
author |
Stachon, Henrique Alexandre |
author_facet |
Stachon, Henrique Alexandre |
author_role |
author |
dc.contributor.none.fl_str_mv |
Urban, Cicero de Andrade http://lattes.cnpq.br/0718187625853159 |
dc.contributor.author.fl_str_mv |
Stachon, Henrique Alexandre |
dc.subject.por.fl_str_mv |
Biotecnologia industrial Infecção Contratura Biofilmes Mastectomia segmentar CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
topic |
Biotecnologia industrial Infecção Contratura Biofilmes Mastectomia segmentar CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Background: Breast is composed of glandular tissue with complex duct-lobular systems. The ductal system flow out of the breast external surface through the areolo-papillary complex. The rates of infection in elective breast surgeries reach values between 3 and 15%, wich are more than expected for operations considered clean and may be associated with manipulation of the areolo-papillary complex and ducts previously colonized by microorganisms. Then, the goal of this study was to investigate if there is a microbiota in the region behind the areola and nipple, where the breast ducts are located, and if it is equal to the microbiota of the skin and to which antibiotics it is sensitive or resistant. Methods: Cultures of breast tissue samples removed from 49 patients who underwent oncologic, aesthetic and reconstructive breast surgeries. Three fragments of breast tissue were removed, about 1 centimeter in diameter each fragment, one retroareolar, lateral, and medial quadrants. Each tissue fragment were transferred into a sterile vial containing BHI broth, and incubated for 7 days at 37°C and in the cases of turbidity of the medium, result of microorganisms growth, will be done fresh blade and sowing in Petry plate for culture and isolation of the strains, antibiogram and identification of the species in automatic reader. Results: There were microorganisms growth in samples from 10 of the 49 patients (20.4%), and 11 of the 218 fragments sampled (5%). The species found were Staphylococcus epidermidis, Staphylococcus lugdunensis, Staphylococcus hominis, Sphingomonas paucimobilis and Aeromonas salmonicida. The presence of these bacteria in about 20% of them could suggest that breast surgeries should be considered as potentially contaminated surgery, and this may have implications for adverse reactions to mammary prosthesis, and also in the genesis of large cell lymphoma associated with prostheses. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018 2021-05-26T13:41:51Z 2021-05-26T13:41:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2269 |
url |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2269 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Biotecnologia Industrial UP |
publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Biotecnologia Industrial UP |
dc.source.none.fl_str_mv |
reponame:Repositório do Centro Universitário Braz Cubas instname:Centro Universitário Braz Cubas (CUB) instacron:CUB |
instname_str |
Centro Universitário Braz Cubas (CUB) |
instacron_str |
CUB |
institution |
CUB |
reponame_str |
Repositório do Centro Universitário Braz Cubas |
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Repositório do Centro Universitário Braz Cubas |
repository.name.fl_str_mv |
Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB) |
repository.mail.fl_str_mv |
bibli@brazcubas.edu.br |
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