Influência da incisão dos ductos mamários na microbiota do leito cirúrgico da mama: avaliação intraoperatória da microbiota da mama

• Autor(a) principal: Stachon, Henrique Alexandre
• Data de Publicação: 2018
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2269
• topic: Biotecnologia industrial; Infecção; Contratura; Biofilmes; Mastectomia segmentar; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Background: Breast is composed of glandular tissue with complex duct-lobular systems. The ductal system flow out of the breast external surface through the areolo-papillary complex. The rates of infection in elective breast surgeries reach values between 3 and 15%, wich are more than expected for operations considered clean and may be associated with manipulation of the areolo-papillary complex and ducts previously colonized by microorganisms. Then, the goal of this study was to investigate if there is a microbiota in the region behind the areola and nipple, where the breast ducts are located, and if it is equal to the microbiota of the skin and to which antibiotics it is sensitive or resistant. Methods: Cultures of breast tissue samples removed from 49 patients who underwent oncologic, aesthetic and reconstructive breast surgeries. Three fragments of breast tissue were removed, about 1 centimeter in diameter each fragment, one retroareolar, lateral, and medial quadrants. Each tissue fragment were transferred into a sterile vial containing BHI broth, and incubated for 7 days at 37°C and in the cases of turbidity of the medium, result of microorganisms growth, will be done fresh blade and sowing in Petry plate for culture and isolation of the strains, antibiogram and identification of the species in automatic reader. Results: There were microorganisms growth in samples from 10 of the 49 patients (20.4%), and 11 of the 218 fragments sampled (5%). The species found were Staphylococcus epidermidis, Staphylococcus lugdunensis, Staphylococcus hominis, Sphingomonas paucimobilis and Aeromonas salmonicida. The presence of these bacteria in about 20% of them could suggest that breast surgeries should be considered as potentially contaminated surgery, and this may have implications for adverse reactions to mammary prosthesis, and also in the genesis of large cell lymphoma associated with prostheses.