Avaliação de polimorfismos genéticos associados ao bruxismo
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório do Centro Universitário Braz Cubas |
Texto Completo: | https://repositorio.cruzeirodosul.edu.br/handle/123456789/2100 |
Resumo: | Introduction: Bruxism is the habit of tooth grinding or clenching while awake and sleeping with at least one sign or symptom. Although bruxism impacts quality of life and is highly prevalent in children and adolescents, its intrinsic etiologic factors (i.e genetic factors) have not been extensively studied in this population yet. Objective: This study was to determine the association of bruxism and circadian manifestations with specific genes that can be helpful to understand the etiology of this disorder in children and adolescents. Methods: One hundred and fifty subjects (7 – 12 years old) were included in this case-control study. DNA was collected from buccal epithelial cells to determine the association between bruxism and FKBP5 (rs1360780 and rs3800373), DRD2 (rs6275 and rs6276), ANKK1 (rs1800497) and COMT (rs6269). Genes polymorphism were investigated through RT-PCR with the TaqMan™ assay in a StepOnePlus System. Logistic univariate regression was used to associate genotypes under additive and dominant models between cases and controls, with significance level of 0.05. Results: In the diagnosis of bruxism, the DRD2 (rs6276) is associated with a higher prevalence in GG homozygous individuals when compared to AA or GA subjects [p = 0.02 / 2.47 (1.13 - 5.36)]. In relation to circadian manifestations of bruxism, the polymorphism ANKK1 is associated with awake teeth grinding (p < 0.001). The DRD2 (rs6276) associated with grinding teeth during sleep in the additive genotypes (p = 0.03) and dominant (p = 0.008). Concerning awake tooth clenching , it had a prevalence in the ANKK1 gene of the TT genotype in both additive (p = 0.05) and dominant (p = 0.08) modes. In sleep tooth clenching , a prevalence of COMT genes in the GG model was found for both additive (p = 0.003) and dominant (p = 0.001). Concerning the aweke tooth clenching, there was association with TT individuals for the ANKK1 gene in the additive model (p = 0.05) and association with the ANKK1 gene (p = 0.08) in the dominant model with the TT gene (p = 0.08). In sleep tooth clenching, was found in the COMT genes in the GG model for both additive (p = 0.003) and dominant (p = 0.001).Conclusion: The DRD2 gene polymorphism is related to bruxism; and ANKK1 and COMT are associated with circadian manifestation of bruxism |
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Avaliação de polimorfismos genéticos associados ao bruxismoOdontologiaBruxismoPolimorfismo (Genética)CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAIntroduction: Bruxism is the habit of tooth grinding or clenching while awake and sleeping with at least one sign or symptom. Although bruxism impacts quality of life and is highly prevalent in children and adolescents, its intrinsic etiologic factors (i.e genetic factors) have not been extensively studied in this population yet. Objective: This study was to determine the association of bruxism and circadian manifestations with specific genes that can be helpful to understand the etiology of this disorder in children and adolescents. Methods: One hundred and fifty subjects (7 – 12 years old) were included in this case-control study. DNA was collected from buccal epithelial cells to determine the association between bruxism and FKBP5 (rs1360780 and rs3800373), DRD2 (rs6275 and rs6276), ANKK1 (rs1800497) and COMT (rs6269). Genes polymorphism were investigated through RT-PCR with the TaqMan™ assay in a StepOnePlus System. Logistic univariate regression was used to associate genotypes under additive and dominant models between cases and controls, with significance level of 0.05. Results: In the diagnosis of bruxism, the DRD2 (rs6276) is associated with a higher prevalence in GG homozygous individuals when compared to AA or GA subjects [p = 0.02 / 2.47 (1.13 - 5.36)]. In relation to circadian manifestations of bruxism, the polymorphism ANKK1 is associated with awake teeth grinding (p < 0.001). The DRD2 (rs6276) associated with grinding teeth during sleep in the additive genotypes (p = 0.03) and dominant (p = 0.008). Concerning awake tooth clenching , it had a prevalence in the ANKK1 gene of the TT genotype in both additive (p = 0.05) and dominant (p = 0.08) modes. In sleep tooth clenching , a prevalence of COMT genes in the GG model was found for both additive (p = 0.003) and dominant (p = 0.001). Concerning the aweke tooth clenching, there was association with TT individuals for the ANKK1 gene in the additive model (p = 0.05) and association with the ANKK1 gene (p = 0.08) in the dominant model with the TT gene (p = 0.08). In sleep tooth clenching, was found in the COMT genes in the GG model for both additive (p = 0.003) and dominant (p = 0.001).Conclusion: The DRD2 gene polymorphism is related to bruxism; and ANKK1 and COMT are associated with circadian manifestation of bruxismIntrodução: O bruxismo é o hábito de ranger ou apertar os dentes acordado ou durante o sono. Embora o bruxismo afete a qualidade de vida e seja altamente prevalente em crianças e adolescentes, seus fatores etiológicos ainda não foram extensivamente estudados nessa população. Objetivo: Determinar se polimorfismos genéticos podem estar associados a etiologia do bruxismo em crianças e adolescentes. Materiais e Métodos: Cento e cinquenta indivíduos (7 - 12 anos) foram incluídos neste estudo casocontrole. O DNA foi coletado de células epiteliais bucais para determinar a associação entre bruxismo e Prolyl Isomerase 5 [FKBP5 (rs1360780 e rs3800373)] , Dopamine Receptor 2 [DRD2 (rs6275 e rs6276)], Ankyrin Repeat And Kinase Domain Containing [ANKK1 (rs1800497)] e Catechol -O- methyltransferase [COMT (rs6269)]. Os polimorfismos genéticos foram avaliados por PCR em tempo real com o ensaio TaqMan™ em um sistema StepOnePlus. A regressão logística univariada foi utilizada para associar genótipos em modelos aditivos e dominantes entre casos e controles, com nível de significância de 0,05 para avaliação dos desfechos de ranger e apertar os dentes foi utilizado o teste de qui-quadrado ou exato de fisher. Resultados: Na perspectiva do diagnóstico de bruxismo, o DRD2 (rs6276) está associado a uma maior prevalência em indivíduos homozigotos GG quando comparados aos indivíduos AA ou GA [p = 0,02 /2,47 (1,13 – 5,36)]. Em relação às manifestações circadianas do bruxismo, o polimorfismo do gene ANKK1 está associado à ranger os dentes em vigília ( p < 0,001). O gene DRD2 (rs6276) está associado com o habito de ranger os dentes durante o sono no modelo aditivo (p = 0,03) e no modelo dominante (p = 0.008). No que se refere ao apertamento dos dentes em vigília, houve associação com indivíduos TT para o gene ANKK1 no modelo aditivo (p = 0,05) e associação com o gene ANKK1 (p = 0,08) no modelo dominante com o gene TT (p = 0,08). No apertar os dentes durante o sono foi encontrado nos genes COMT no modelo GG tanto para aditivo (p = 0,003) e dominante (p = 0,001). Conclusão: O polimorfismo do gene DRD2 está relacionado ao bruxismo e a ANKK1 e a COMT estão associadas à manifestação circadiana do bruxismo.Universidade PositivoBrasilPós-GraduaçãoPrograma de Pós-Graduação em Odontologia ClínicaUPZielak, João Cesarhttp://lattes.cnpq.br/5991949730989569Scariot, Rafaelahttp://lattes.cnpq.br/8726711027143249Savaris, Leonardo Brunet2021-05-07T22:49:42Z20192021-05-07T22:49:42Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://repositorio.cruzeirodosul.edu.br/handle/123456789/2100porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2021-06-17T17:57:29Zoai:repositorio.cruzeirodosul.edu.br:123456789/2100Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2021-06-17T17:57:29Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false |
dc.title.none.fl_str_mv |
Avaliação de polimorfismos genéticos associados ao bruxismo |
title |
Avaliação de polimorfismos genéticos associados ao bruxismo |
spellingShingle |
Avaliação de polimorfismos genéticos associados ao bruxismo Savaris, Leonardo Brunet Odontologia Bruxismo Polimorfismo (Genética) CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Avaliação de polimorfismos genéticos associados ao bruxismo |
title_full |
Avaliação de polimorfismos genéticos associados ao bruxismo |
title_fullStr |
Avaliação de polimorfismos genéticos associados ao bruxismo |
title_full_unstemmed |
Avaliação de polimorfismos genéticos associados ao bruxismo |
title_sort |
Avaliação de polimorfismos genéticos associados ao bruxismo |
author |
Savaris, Leonardo Brunet |
author_facet |
Savaris, Leonardo Brunet |
author_role |
author |
dc.contributor.none.fl_str_mv |
Zielak, João Cesar http://lattes.cnpq.br/5991949730989569 Scariot, Rafaela http://lattes.cnpq.br/8726711027143249 |
dc.contributor.author.fl_str_mv |
Savaris, Leonardo Brunet |
dc.subject.por.fl_str_mv |
Odontologia Bruxismo Polimorfismo (Genética) CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
topic |
Odontologia Bruxismo Polimorfismo (Genética) CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Introduction: Bruxism is the habit of tooth grinding or clenching while awake and sleeping with at least one sign or symptom. Although bruxism impacts quality of life and is highly prevalent in children and adolescents, its intrinsic etiologic factors (i.e genetic factors) have not been extensively studied in this population yet. Objective: This study was to determine the association of bruxism and circadian manifestations with specific genes that can be helpful to understand the etiology of this disorder in children and adolescents. Methods: One hundred and fifty subjects (7 – 12 years old) were included in this case-control study. DNA was collected from buccal epithelial cells to determine the association between bruxism and FKBP5 (rs1360780 and rs3800373), DRD2 (rs6275 and rs6276), ANKK1 (rs1800497) and COMT (rs6269). Genes polymorphism were investigated through RT-PCR with the TaqMan™ assay in a StepOnePlus System. Logistic univariate regression was used to associate genotypes under additive and dominant models between cases and controls, with significance level of 0.05. Results: In the diagnosis of bruxism, the DRD2 (rs6276) is associated with a higher prevalence in GG homozygous individuals when compared to AA or GA subjects [p = 0.02 / 2.47 (1.13 - 5.36)]. In relation to circadian manifestations of bruxism, the polymorphism ANKK1 is associated with awake teeth grinding (p < 0.001). The DRD2 (rs6276) associated with grinding teeth during sleep in the additive genotypes (p = 0.03) and dominant (p = 0.008). Concerning awake tooth clenching , it had a prevalence in the ANKK1 gene of the TT genotype in both additive (p = 0.05) and dominant (p = 0.08) modes. In sleep tooth clenching , a prevalence of COMT genes in the GG model was found for both additive (p = 0.003) and dominant (p = 0.001). Concerning the aweke tooth clenching, there was association with TT individuals for the ANKK1 gene in the additive model (p = 0.05) and association with the ANKK1 gene (p = 0.08) in the dominant model with the TT gene (p = 0.08). In sleep tooth clenching, was found in the COMT genes in the GG model for both additive (p = 0.003) and dominant (p = 0.001).Conclusion: The DRD2 gene polymorphism is related to bruxism; and ANKK1 and COMT are associated with circadian manifestation of bruxism |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019 2021-05-07T22:49:42Z 2021-05-07T22:49:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2100 |
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https://repositorio.cruzeirodosul.edu.br/handle/123456789/2100 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Odontologia Clínica UP |
publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Odontologia Clínica UP |
dc.source.none.fl_str_mv |
reponame:Repositório do Centro Universitário Braz Cubas instname:Centro Universitário Braz Cubas (CUB) instacron:CUB |
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Centro Universitário Braz Cubas (CUB) |
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CUB |
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Repositório do Centro Universitário Braz Cubas |
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Repositório do Centro Universitário Braz Cubas |
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Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB) |
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bibli@brazcubas.edu.br |
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1798311338831773696 |