Avaliação da possível mutagenicidade do extrato de própolis brasileira em formulações tópicas em células de mamíferos in vivo e in vitro

• Autor(a) principal: Senedese, Juliana Marques
• Data de Publicação: 2007
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/822
• topic: Própolis; Micronúcleo; Aberrações cromossômicas; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA

Propolis is a resinous substance with coloring and consistence related to flowers visited by bees. Propolis is produced from vegetal resins taken into the hives where bees add their own secretions to it. Propolis usage dates back ancient times, with a wide biological activity, such as antioxidant, antibiotic, anticancer, antiinflammatory, among others. The present study evaluated the genotoxic potential of topical formulations supplemented with propolis extract (1.2, 2.4 and 3.6%) for the treatment of burns produced by Apis Flora Industrial e Comercial Ltda., having as a parameter the analysis of micronucleus in peripheral blood Wistar rats and analysis of chromosomal aberrations in Chinese hamster ovary cells. In the in vivo assessment, the animals were injured on the back and then submitted to subacute (7 days) and subchronic (30 days) treatments consisting of daily dermal applications of gels containing different concentrations of propolis. In the in vitro studies, 3-h pulse (G1 phase of the cell cycle) and continuous (20 h) treatments were performed. No statistically significant differences in the frequencies of micronucleus were observed between animals treated with gels containing different concentrations of propolis and the negative control for the two treatment times. Similar frequencies of chromosome aberrations were observed for cultures submitted to 3-h pulse and continuous treatment with gels containing different propolis concentrations and cultures not submitted to any treatment. However, in the continuous treatment cultures treated with the 3.6% propolis gel presented significantly lower mitotic indices than the negative control. Under the present conditions, topical formulations containing different concentrations of propolis used for the treatment of burns showed no clastogenic effect in either test system, but 3.6% propolis gel was found to be cytotoxic in the in vitro test. Keywords: micronuclei, propolis, chromosomal aberrations