Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleo

Detalhes bibliográficos
Autor(a) principal: Silva, Brício Marcelino da
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório do Centro Universitário Braz Cubas
Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2528
Resumo: Oil is the main source of energy today and is also the cause of several environmental problems. Therefore, there is a need to develop strategies to combat contamination caused by daily use, as well as from the accidents and those resulting from oil extraction operations. In order to aid in the improvement of biological strategies to combat oil contamination, it was possible to prospect, isolate and identify bacteria associated with rhizosphere of Sporobolus virginicus (L.) Kunth collected in environments subject to oil contamination. Six samples (root and rhizospheric soil) were collected from four sites on the northern coast of the state of Rio de Janeiro, Brazil, totaling 24 samples. Extraction of the microorganisms resulted in the isolation of 46 morphotypes. Those in which colony forming units (CFUs) were observed in more than 2.5% (n = 336) of the total plaques were selected for identification. Fifteen morphotypes with different morphologies were characterized by means of Gram staining, of which 12 were Grampositive and three Gram-negative. Identification through genomic sequencing revealed that Bacillaceae (73.7%), Staphylococcaceae (6.7%), Xanthomonadaceae (6.7%) and Enterobacteriaceae (13,4%) are bacteria belonging to four families. Exiguobacterium sp., Staphylococcus sp., Pantoea sp., Stenotrophomonas sp. and Serratia sp. had their oil degradation efficiency (ED) of petroleum (4% v / v) measured, for up to 96 hours. The pH, the electrical conductivity and the dissolved oxygen concentration were monitored. Cellular concentration was estimated by absorbance and biomass increase measured by gravimetry. In the time intervals of higher cell concentration samples were collected to evaluate the efficiency of the oil degradation by gas chromatography with flame ionization detector (CG-FID). The results suggest that there was degradation of the crude oil promoted by the bacteria Exiguobacterium sp., Staphylococcus sp., Pantoea sp., Stenotrophomonas sp. and Serratia sp. verified from the chromatograms, where a reduction in the area of some peaks in different retention times (RT) was observed. The largest reduction measured was for Stenotrophomonas sp. with the value of 73.12% and the lowest for Exiguobacterium sp. with the value of 6.8%. These results broaden the knowledge about the use of the microbial community for the biodegradation of petroleum and petroderivatives, which may allow environmental managers to diversify the strategies that can be applied to techniques for recovering contaminated areas, particularly in coastal environments.
id CUB_6bd0c48180527939b1fbb8a5efcd4915
oai_identifier_str oai:repositorio.cruzeirodosul.edu.br:123456789/2528
network_acronym_str CUB
network_name_str Repositório do Centro Universitário Braz Cubas
repository_id_str
spelling Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleoGestão ambientalÁgua - Poluição por petróleoHidrocarbonetosRecuperação e remediação ambientalBiorremediaçãoCNPQ::CIENCIAS BIOLOGICASCNPQ::ENGENHARIAS::ENGENHARIA SANITARIAOil is the main source of energy today and is also the cause of several environmental problems. Therefore, there is a need to develop strategies to combat contamination caused by daily use, as well as from the accidents and those resulting from oil extraction operations. In order to aid in the improvement of biological strategies to combat oil contamination, it was possible to prospect, isolate and identify bacteria associated with rhizosphere of Sporobolus virginicus (L.) Kunth collected in environments subject to oil contamination. Six samples (root and rhizospheric soil) were collected from four sites on the northern coast of the state of Rio de Janeiro, Brazil, totaling 24 samples. Extraction of the microorganisms resulted in the isolation of 46 morphotypes. Those in which colony forming units (CFUs) were observed in more than 2.5% (n = 336) of the total plaques were selected for identification. Fifteen morphotypes with different morphologies were characterized by means of Gram staining, of which 12 were Grampositive and three Gram-negative. Identification through genomic sequencing revealed that Bacillaceae (73.7%), Staphylococcaceae (6.7%), Xanthomonadaceae (6.7%) and Enterobacteriaceae (13,4%) are bacteria belonging to four families. Exiguobacterium sp., Staphylococcus sp., Pantoea sp., Stenotrophomonas sp. and Serratia sp. had their oil degradation efficiency (ED) of petroleum (4% v / v) measured, for up to 96 hours. The pH, the electrical conductivity and the dissolved oxygen concentration were monitored. Cellular concentration was estimated by absorbance and biomass increase measured by gravimetry. In the time intervals of higher cell concentration samples were collected to evaluate the efficiency of the oil degradation by gas chromatography with flame ionization detector (CG-FID). The results suggest that there was degradation of the crude oil promoted by the bacteria Exiguobacterium sp., Staphylococcus sp., Pantoea sp., Stenotrophomonas sp. and Serratia sp. verified from the chromatograms, where a reduction in the area of some peaks in different retention times (RT) was observed. The largest reduction measured was for Stenotrophomonas sp. with the value of 73.12% and the lowest for Exiguobacterium sp. with the value of 6.8%. These results broaden the knowledge about the use of the microbial community for the biodegradation of petroleum and petroderivatives, which may allow environmental managers to diversify the strategies that can be applied to techniques for recovering contaminated areas, particularly in coastal environments.O petróleo é a principal fonte de energia da atualidade e é também o causador de diversos problemas ambientais. Por isso, há a necessidade do desenvolvimento de estratégias de combate à contaminação ocasionada pela utilização diária, bem como às provenientes dos acidentes e às decorrentes das operações de extração do petróleo. Com o objetivo de auxiliar no aprimoramento de estratégias biológicas de combate à contaminação por petróleo foi feita a prospecção, isolamento e identificação de bactérias associadas à rizosfera de Sporobolus virginicus (L.) Kunth coletadas em ambientes sujeitos à contaminação com petróleo. Foram coletadas seis amostras (raiz e solo rizosférico) de quatro pontos do litoral norte do estado do Rio de Janeiro, Brasil, totalizando 24 amostras. A extração dos micro-organismos resultou no isolamento de 46 morfotipos. Aqueles em que as unidades formadoras de colônias (UFCs) foram observados em mais de 2,5% (n=336) do total de placas foram selecionados para identificação. Quinze morfotipos foram caracterizados por meio de Coloração de Gram, sendo que 12 são Gram-positivos e três Gram-negativos. A identificação por meio de sequenciamento genômico revelou que são bactérias pertencentes a quatro famílias: Bacillaceae (73,7%), Staphylococcaceae (6,7%), Xanthomonadaceae (6,7%) e Enterobacteriaceae (13,4%). Exiguobacterium sp., Staphylococcus sp., Pantoea sp., Stenotrophomonas sp. e Serratia sp. tiveram sua eficiência de degradação (ED) de petróleo (4% v/v) medidas, por até 96 horas. Foram monitorados o pH, a condutividade elétrica e a concentração de oxigênio dissolvido. A concentração celular foi estimada por absorbância e o aumento de biomassa por gravimetria. Nos intervalos de tempo de maior concentração celular foram coletadas amostras para avaliar a eficiência da degradação de petróleo por cromatografia em fase gasosa com detector por ionização de chama (CG-FID). Os resultados sugerem que houve degradação do petróleo bruto promovido pelas bactérias Exiguobacterium sp., Staphylococcus sp., Pantoea sp., Stenotrophomonas sp. e Serratia sp. Nos cromatogramas, foram observadas reduções na área de alguns picos em diferentes tempos de retenção (TR). A maior redução medida foi para Stenotrophomonas sp., no TR de 5,092 min (73,12%) e a menor para Exiguobacterium sp., no TR de 19,262 min (6,80%). Esses resultados ampliam o conhecimento sobre o emprego da comunidade microbiana para biodegradação de petróleo e petroderivados, o que pode possibilitar, aos gestores ambientais, a diversificação das estratégias que podem ser aplicadas em técnicas de recuperação de áreas contaminadas, particularmente, em ambientes costeiros.Universidade PositivoBrasilPós-GraduaçãoPrograma de Pós-Graduação em Gestão AmbientalUPMaranho, Leila Teresinhahttp://lattes.cnpq.br/3269388039410529Silva, Brício Marcelino da2021-08-11T18:40:16Z20172021-08-11T18:40:16Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://repositorio.cruzeirodosul.edu.br/handle/123456789/2528porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2021-08-12T13:14:10Zoai:repositorio.cruzeirodosul.edu.br:123456789/2528Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2021-08-12T13:14:10Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false
dc.title.none.fl_str_mv Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleo
title Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleo
spellingShingle Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleo
Silva, Brício Marcelino da
Gestão ambiental
Água - Poluição por petróleo
Hidrocarbonetos
Recuperação e remediação ambiental
Biorremediação
CNPQ::CIENCIAS BIOLOGICAS
CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA
title_short Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleo
title_full Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleo
title_fullStr Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleo
title_full_unstemmed Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleo
title_sort Prospecção e avaliação do potencial de degradação de petróleo por bactérias biodegradadoras extraídas da rizosfera de Sporobolus virginicus (L.) Kunth coletada em área litorânea sujeita à contaminação por petróleo
author Silva, Brício Marcelino da
author_facet Silva, Brício Marcelino da
author_role author
dc.contributor.none.fl_str_mv Maranho, Leila Teresinha
http://lattes.cnpq.br/3269388039410529
dc.contributor.author.fl_str_mv Silva, Brício Marcelino da
dc.subject.por.fl_str_mv Gestão ambiental
Água - Poluição por petróleo
Hidrocarbonetos
Recuperação e remediação ambiental
Biorremediação
CNPQ::CIENCIAS BIOLOGICAS
CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA
topic Gestão ambiental
Água - Poluição por petróleo
Hidrocarbonetos
Recuperação e remediação ambiental
Biorremediação
CNPQ::CIENCIAS BIOLOGICAS
CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA
description Oil is the main source of energy today and is also the cause of several environmental problems. Therefore, there is a need to develop strategies to combat contamination caused by daily use, as well as from the accidents and those resulting from oil extraction operations. In order to aid in the improvement of biological strategies to combat oil contamination, it was possible to prospect, isolate and identify bacteria associated with rhizosphere of Sporobolus virginicus (L.) Kunth collected in environments subject to oil contamination. Six samples (root and rhizospheric soil) were collected from four sites on the northern coast of the state of Rio de Janeiro, Brazil, totaling 24 samples. Extraction of the microorganisms resulted in the isolation of 46 morphotypes. Those in which colony forming units (CFUs) were observed in more than 2.5% (n = 336) of the total plaques were selected for identification. Fifteen morphotypes with different morphologies were characterized by means of Gram staining, of which 12 were Grampositive and three Gram-negative. Identification through genomic sequencing revealed that Bacillaceae (73.7%), Staphylococcaceae (6.7%), Xanthomonadaceae (6.7%) and Enterobacteriaceae (13,4%) are bacteria belonging to four families. Exiguobacterium sp., Staphylococcus sp., Pantoea sp., Stenotrophomonas sp. and Serratia sp. had their oil degradation efficiency (ED) of petroleum (4% v / v) measured, for up to 96 hours. The pH, the electrical conductivity and the dissolved oxygen concentration were monitored. Cellular concentration was estimated by absorbance and biomass increase measured by gravimetry. In the time intervals of higher cell concentration samples were collected to evaluate the efficiency of the oil degradation by gas chromatography with flame ionization detector (CG-FID). The results suggest that there was degradation of the crude oil promoted by the bacteria Exiguobacterium sp., Staphylococcus sp., Pantoea sp., Stenotrophomonas sp. and Serratia sp. verified from the chromatograms, where a reduction in the area of some peaks in different retention times (RT) was observed. The largest reduction measured was for Stenotrophomonas sp. with the value of 73.12% and the lowest for Exiguobacterium sp. with the value of 6.8%. These results broaden the knowledge about the use of the microbial community for the biodegradation of petroleum and petroderivatives, which may allow environmental managers to diversify the strategies that can be applied to techniques for recovering contaminated areas, particularly in coastal environments.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2021-08-11T18:40:16Z
2021-08-11T18:40:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.cruzeirodosul.edu.br/handle/123456789/2528
url https://repositorio.cruzeirodosul.edu.br/handle/123456789/2528
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Positivo
Brasil
Pós-Graduação
Programa de Pós-Graduação em Gestão Ambiental
UP
publisher.none.fl_str_mv Universidade Positivo
Brasil
Pós-Graduação
Programa de Pós-Graduação em Gestão Ambiental
UP
dc.source.none.fl_str_mv reponame:Repositório do Centro Universitário Braz Cubas
instname:Centro Universitário Braz Cubas (CUB)
instacron:CUB
instname_str Centro Universitário Braz Cubas (CUB)
instacron_str CUB
institution CUB
reponame_str Repositório do Centro Universitário Braz Cubas
collection Repositório do Centro Universitário Braz Cubas
repository.name.fl_str_mv Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)
repository.mail.fl_str_mv bibli@brazcubas.edu.br
_version_ 1798311349630009344