Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronato
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório do Centro Universitário Braz Cubas |
Texto Completo: | https://repositorio.cruzeirodosul.edu.br/handle/123456789/2241 |
Resumo: | Little is known about the effects of alendronate during bone development in neonatal period. Some studies show that drug interfering in bone growth factors, favoring the development of this tissue. Supported by this premise, the objective of this study, is verify the immunoexpression of TGF-β1 and BMP-2 in maxillary and femoral bone development in specimes that received alendronate. Sixty neonatal rats were used, 30 of them received the administration via intraperitoneal of Alendronate 1mg/kg/daily, while the other rats constitutes the control group (received saline solution). After 2, 7 and 12 day after birthday the rats were euthanatized and the femur and skull was collected to histological and immunohistochemical analysis of expression of TGF-1and BMP-2 and histological analyses. On skull in group Aln with 7 days of life it was possible to observe fusion on the secondary palate, in the whole group, however, a fusion interface area of the primary palate remained open in both groups. In addition, a presence of bone matrix deposition was more dense when compared to the control. These results coincided with higher immunoexpression of TGF-β1 and BMP-2. On day 12, both group ,C and Aln, presented fusion of the secondary palate. However, the primary palate remained open. The control group presented greater mineralization in this anatomical area due to a smaller endochondral expansion. In the femur on day 2, there was difference between group C and Aln. group C , the entire cartilage area exhibited positivity to TGF-β1, whereas a BMP-2 was positive for the recently produced peripheral trabecular bone. In the Aln group TGF-β1 was expressed in some sites of neoformed trabecular bone, peripherally to the bone marrow area. BMP-2, unusually, was positive in proliferative and hypertrophic chondrocytes. On day 12, as immunohistochemical characteristics, as well as histological characteristics of group C, were similar to group C with 2 days. Differently, all as chondrocyte layers, positively exhibited a BMP-2 in specimens receiving alendronate promoting atypical and irregular endochondral deposition, minimizing chondroblastic expansion. The newly formed bone also showed to be different at 12 days in the Aln group. Within the limits of the present study, it was possible to conclude: In palate the alendronate only accelerated the closure of the secondary palate, and not mineralization between the primary palate. In the femoral area, alendronate produced atypical bone formation with serial cartilage, prompting that this drug may compromise the growth zone of the long bones. |
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Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronatoOdontologiaAlendronatoDesenvolvimento ósseoOsteogêneseCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIALittle is known about the effects of alendronate during bone development in neonatal period. Some studies show that drug interfering in bone growth factors, favoring the development of this tissue. Supported by this premise, the objective of this study, is verify the immunoexpression of TGF-β1 and BMP-2 in maxillary and femoral bone development in specimes that received alendronate. Sixty neonatal rats were used, 30 of them received the administration via intraperitoneal of Alendronate 1mg/kg/daily, while the other rats constitutes the control group (received saline solution). After 2, 7 and 12 day after birthday the rats were euthanatized and the femur and skull was collected to histological and immunohistochemical analysis of expression of TGF-1and BMP-2 and histological analyses. On skull in group Aln with 7 days of life it was possible to observe fusion on the secondary palate, in the whole group, however, a fusion interface area of the primary palate remained open in both groups. In addition, a presence of bone matrix deposition was more dense when compared to the control. These results coincided with higher immunoexpression of TGF-β1 and BMP-2. On day 12, both group ,C and Aln, presented fusion of the secondary palate. However, the primary palate remained open. The control group presented greater mineralization in this anatomical area due to a smaller endochondral expansion. In the femur on day 2, there was difference between group C and Aln. group C , the entire cartilage area exhibited positivity to TGF-β1, whereas a BMP-2 was positive for the recently produced peripheral trabecular bone. In the Aln group TGF-β1 was expressed in some sites of neoformed trabecular bone, peripherally to the bone marrow area. BMP-2, unusually, was positive in proliferative and hypertrophic chondrocytes. On day 12, as immunohistochemical characteristics, as well as histological characteristics of group C, were similar to group C with 2 days. Differently, all as chondrocyte layers, positively exhibited a BMP-2 in specimens receiving alendronate promoting atypical and irregular endochondral deposition, minimizing chondroblastic expansion. The newly formed bone also showed to be different at 12 days in the Aln group. Within the limits of the present study, it was possible to conclude: In palate the alendronate only accelerated the closure of the secondary palate, and not mineralization between the primary palate. In the femoral area, alendronate produced atypical bone formation with serial cartilage, prompting that this drug may compromise the growth zone of the long bones.Pouco sabe-se sobre os efeitos do alendronato durante o desenvolvimento ósseo no período neonatal. Alguns estudos apontam que esse fármaco pode interferir nos fatores de crescimento ósseo, favorecendo o desenvolvimento deste tecido. Nessa perspectiva, o objetivo do presente estudo foi avaliar, através da imunoexpressão do TGF-β1 e BMP-2, os efeitos do alendronato de sódio no desenvolvimento ósseo maxilar e femoral em ratos neonatos. Foram utilizados 60 ratos neonatos, os quais foram alocados aleatoriamente em dois grupos, o controle (C) e o teste (Aln). Os grupos foram divididos em três subgrupos para eutanásia em 2, 7 e 12 dias de vida. No grupo C foi administrado soro fisiológico na dosagem de 1ml/Kg de solução. E o grupo Aln recebeu aplicação de alendronato de sódio, na dosagem de 2,5 mg/Kg de massa corpórea. Após a eutanásia foram realizadas as análises imunohistoquímicas através da expressão de TGF-β1 e BMP-2 e histológica. No palato com 7 dias foi possível observar que na área de palato secundário, todo o grupo que recebeu alendronato apresentou fusão nesta topografia, contudo a área de interface de fusão dos palatos primários permaneceu aberta em ambos os grupos. Além disso, a presença de deposição de matriz óssea foi mais densa quando comparada ao controle. Esses efeitos coincidiram com maior imunoexpressão do TGF-β1 e BMP-2. No 12º dia, tanto o grupo C quanto o Aln, apresentaram fusão do palato secundário. Contudo, embora os palatos primários continuassem abertos, o grupo controle apresentou maior mineralização nesta área anatômica devido a menor expansão endocondral. Já no fêmur no 2º dia houve diferença entre o grupo C e Aln. No grupo C, toda área de cartilagem exibiu positividade ao para o TGF-β1, enquanto a BMP-2 foi positiva ao osso trabecular periférico recentemente produzido. No grupo Aln o TGF-β1 foi expresso em alguns locais de osso trabecular neoformado, perifericamente para a área da medula óssea. O BMP-2, de forma incomum, foi positivo em condrócitos proliferativos e hipertrópicos. No 12º dia, as características imunohistoquímica, bem como características histológicas do grupo C, foram semelhantes ao grupo C com 2 dias. Diferentemente, todas as camadas de condrócitos, exibiram positivamente a BMP-2 em espécimes que receberam alendronato promovendo uma deposição endocondral atípica e irregular, minimizando a expansão condroblástica. O osso neoformado também mostrou ser diferente aos 12 dias no grupo Aln. Dentro dos limites do presente estudo, foi possível concluir que: No palato o alendronato acelerou apenas o fechamento do palato secundário, enquanto artrasou a mineralização entre os palatos primários. Na área femoral, o alendronato produziu formação de osso atípico no interior da cartilagem seriada, incitando que este fármaco pode comprometer a zona de crescimento dos ossos longos.Universidade PositivoBrasilPós-GraduaçãoPrograma de Pós-Graduação em Odontologia ClínicaUPGiovanini, Allan Fernandohttp://lattes.cnpq.br/8065373057528197Gonzaga, Carla GastigliaVieira, Juliana de Souza2021-05-24T21:01:36Z20172021-05-24T21:01:36Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://repositorio.cruzeirodosul.edu.br/handle/123456789/2241porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2021-06-23T13:02:18Zoai:repositorio.cruzeirodosul.edu.br:123456789/2241Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2021-06-23T13:02:18Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false |
dc.title.none.fl_str_mv |
Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronato |
title |
Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronato |
spellingShingle |
Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronato Vieira, Juliana de Souza Odontologia Alendronato Desenvolvimento ósseo Osteogênese CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronato |
title_full |
Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronato |
title_fullStr |
Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronato |
title_full_unstemmed |
Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronato |
title_sort |
Análise da imunoexpressão do TGF-β1 e BMP-2 no desenvolvimento craniofacial e femoral de ratos neonatos tratados e não tratados com alendronato |
author |
Vieira, Juliana de Souza |
author_facet |
Vieira, Juliana de Souza |
author_role |
author |
dc.contributor.none.fl_str_mv |
Giovanini, Allan Fernando http://lattes.cnpq.br/8065373057528197 Gonzaga, Carla Gastiglia |
dc.contributor.author.fl_str_mv |
Vieira, Juliana de Souza |
dc.subject.por.fl_str_mv |
Odontologia Alendronato Desenvolvimento ósseo Osteogênese CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
topic |
Odontologia Alendronato Desenvolvimento ósseo Osteogênese CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Little is known about the effects of alendronate during bone development in neonatal period. Some studies show that drug interfering in bone growth factors, favoring the development of this tissue. Supported by this premise, the objective of this study, is verify the immunoexpression of TGF-β1 and BMP-2 in maxillary and femoral bone development in specimes that received alendronate. Sixty neonatal rats were used, 30 of them received the administration via intraperitoneal of Alendronate 1mg/kg/daily, while the other rats constitutes the control group (received saline solution). After 2, 7 and 12 day after birthday the rats were euthanatized and the femur and skull was collected to histological and immunohistochemical analysis of expression of TGF-1and BMP-2 and histological analyses. On skull in group Aln with 7 days of life it was possible to observe fusion on the secondary palate, in the whole group, however, a fusion interface area of the primary palate remained open in both groups. In addition, a presence of bone matrix deposition was more dense when compared to the control. These results coincided with higher immunoexpression of TGF-β1 and BMP-2. On day 12, both group ,C and Aln, presented fusion of the secondary palate. However, the primary palate remained open. The control group presented greater mineralization in this anatomical area due to a smaller endochondral expansion. In the femur on day 2, there was difference between group C and Aln. group C , the entire cartilage area exhibited positivity to TGF-β1, whereas a BMP-2 was positive for the recently produced peripheral trabecular bone. In the Aln group TGF-β1 was expressed in some sites of neoformed trabecular bone, peripherally to the bone marrow area. BMP-2, unusually, was positive in proliferative and hypertrophic chondrocytes. On day 12, as immunohistochemical characteristics, as well as histological characteristics of group C, were similar to group C with 2 days. Differently, all as chondrocyte layers, positively exhibited a BMP-2 in specimens receiving alendronate promoting atypical and irregular endochondral deposition, minimizing chondroblastic expansion. The newly formed bone also showed to be different at 12 days in the Aln group. Within the limits of the present study, it was possible to conclude: In palate the alendronate only accelerated the closure of the secondary palate, and not mineralization between the primary palate. In the femoral area, alendronate produced atypical bone formation with serial cartilage, prompting that this drug may compromise the growth zone of the long bones. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017 2021-05-24T21:01:36Z 2021-05-24T21:01:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2241 |
url |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2241 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Odontologia Clínica UP |
publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Odontologia Clínica UP |
dc.source.none.fl_str_mv |
reponame:Repositório do Centro Universitário Braz Cubas instname:Centro Universitário Braz Cubas (CUB) instacron:CUB |
instname_str |
Centro Universitário Braz Cubas (CUB) |
instacron_str |
CUB |
institution |
CUB |
reponame_str |
Repositório do Centro Universitário Braz Cubas |
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Repositório do Centro Universitário Braz Cubas |
repository.name.fl_str_mv |
Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB) |
repository.mail.fl_str_mv |
bibli@brazcubas.edu.br |
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1798311337407807488 |