Produção de biomassa de levedura produtora de carotenoide aplicando glicerol bruto residual da síntese de biodisel como substrato

• Autor(a) principal: Scherner, Maria Gabriela
• Data de Publicação: 2020
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2781
• topic: Biotecnologia; Óleos vegetais como combustível; Combustíveis – subprodutos; Carotenóides; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

The possibility of crises in the extraction and sale of oil, the monopoly of large producers that define the commercial domain of their product, and a growing environmental thought in favor of the use of renewable or biodegradable materials, makes it necessary to search for new inputs that can be incorporated into the market. Biodiesel can be a biofuel that meets the needs of mechanized and automotive industries, due to its performance similar to petroleum products. However, this fuel oil, derived from vegetable, animal, or residual oils, brings in its production line a problem linked to the manufacture of a large residue, glycerol, responsible for a volume of about 10% of the final production of the production plants. biodiesel. Because it is a material with great possibilities for industrial implementation, due to its malleability as raw material, incineration does not become a profitable alternative, allowing the search for new processing of this substance, reconciling the monetary yield from new products to an eco-sustainable alternative. . This work aims to use crude glycerol, without previous treatments, in fermentative processes for the production of added value biomass, using yeasts that support this material as a substrate without a high level of toxicity. Fermentation tests in Erlenmeyer flasks were used to make it possible to determine optimal fermentation conditions and to substantiate the work when placed in a bench bioreactor. All procedures were applied in triplicate, to accumulate data, aiming at statistical calculations of cell growth configurations, using the sampling strategy every two hours for activities between 72 and 84 hours. Using 3% glycerol for Rhodotoula glutinis and 5% for Sporobolomyces ruberrimus, the biomass growth, analyzed from gravimetry, resulted in na Xmáx de 6,78 g.L-1 e Xmáx de 6,44 g.L-1 respectively.